[Clostridium] populeti

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6KQ85|A0A1I6KQ85_9FIRM Uncharacterized protein OS=[Clostridium] populeti OX=37658 GN=SAMN05661086_02574 PE=4 SV=1
MM1 pKa = 7.6FDD3 pKa = 4.2SFFKK7 pKa = 10.75FAGANGDD14 pKa = 3.77TFKK17 pKa = 11.45SFDD20 pKa = 3.34EE21 pKa = 4.53FMNTVSNLPTNFDD34 pKa = 4.0FNALDD39 pKa = 3.73TNGDD43 pKa = 3.78GVISLSEE50 pKa = 3.9FMNATSNLPISTDD63 pKa = 3.79FTSFDD68 pKa = 4.16ANGDD72 pKa = 3.62GYY74 pKa = 11.54LSVDD78 pKa = 3.49EE79 pKa = 4.96CNHH82 pKa = 6.32LFSSMPLNFNFNAADD97 pKa = 3.79KK98 pKa = 10.98DD99 pKa = 3.71HH100 pKa = 7.48DD101 pKa = 4.39GFLSMDD107 pKa = 3.15EE108 pKa = 4.17FMNAKK113 pKa = 10.3ASLTQEE119 pKa = 3.82FAVGYY124 pKa = 8.3EE125 pKa = 3.89INPSINHH132 pKa = 7.09ADD134 pKa = 3.85TISFTGGASCLSSSHH149 pKa = 6.19ISSVTNACVNGGYY162 pKa = 9.71VVSS165 pKa = 4.29

Molecular weight: 17.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6LPA7|A0A1I6LPA7_9FIRM Amino acid permease OS=[Clostridium] populeti OX=37658 GN=SAMN05661086_03443 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.92VHH16 pKa = 6.1GFRR19 pKa = 11.84NRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.91VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3680

0

3680

1277886

39

7265

347.3

39.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.342 ± 0.042

1.442 ± 0.014

5.235 ± 0.026

7.812 ± 0.05

4.275 ± 0.025

6.528 ± 0.048

1.571 ± 0.016

8.263 ± 0.042

7.519 ± 0.04

8.782 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.02

5.234 ± 0.033

2.972 ± 0.027

3.447 ± 0.026

3.615 ± 0.033

6.409 ± 0.039

5.575 ± 0.042

6.824 ± 0.035

0.894 ± 0.015

4.439 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski