Peruvian horse sickness virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2Q1D9|Q2Q1D9_9REOV VP6 OS=Peruvian horse sickness virus OX=356862 PE=4 SV=1
MM1 pKa = 7.27GGIYY5 pKa = 10.42ARR7 pKa = 11.84TFCYY11 pKa = 10.48LEE13 pKa = 4.3CLASNRR19 pKa = 11.84DD20 pKa = 3.11PRR22 pKa = 11.84ARR24 pKa = 11.84RR25 pKa = 11.84TFLPSDD31 pKa = 4.27GFPLFVTRR39 pKa = 11.84FNATTNRR46 pKa = 11.84PITQTPSTPEE56 pKa = 3.37EE57 pKa = 3.97HH58 pKa = 6.85RR59 pKa = 11.84ACFYY63 pKa = 11.24AALDD67 pKa = 4.08LLIGATGINFNYY79 pKa = 10.16RR80 pKa = 11.84LPEE83 pKa = 3.87YY84 pKa = 10.35SPNVQVLSILARR96 pKa = 11.84DD97 pKa = 3.85DD98 pKa = 4.38LPYY101 pKa = 10.35TSEE104 pKa = 3.84AFIRR108 pKa = 11.84ATRR111 pKa = 11.84ITNEE115 pKa = 3.68TAAGQSTRR123 pKa = 11.84RR124 pKa = 11.84FNLPWPDD131 pKa = 3.21VNFIYY136 pKa = 10.35PPATPYY142 pKa = 9.65RR143 pKa = 11.84TVDD146 pKa = 3.66DD147 pKa = 4.16QPQPMVIGPQDD158 pKa = 3.41MEE160 pKa = 3.64ITLNVNEE167 pKa = 4.9DD168 pKa = 3.62VEE170 pKa = 4.37ITDD173 pKa = 3.27IVMPNYY179 pKa = 9.54PDD181 pKa = 4.02VINTCFVWYY190 pKa = 8.75TLSHH194 pKa = 5.47YY195 pKa = 10.41RR196 pKa = 11.84GAAGALVEE204 pKa = 5.53GSQQCSVTAGDD215 pKa = 4.22EE216 pKa = 4.15EE217 pKa = 5.09AEE219 pKa = 4.28SGVEE223 pKa = 3.96IIVPAGTAIRR233 pKa = 11.84VSNTSAVYY241 pKa = 10.68AGMIHH246 pKa = 7.08FNIRR250 pKa = 11.84WFTRR254 pKa = 11.84AQPSEE259 pKa = 4.15DD260 pKa = 4.36LYY262 pKa = 11.3DD263 pKa = 3.54TMRR266 pKa = 11.84VDD268 pKa = 4.88FAASYY273 pKa = 10.67SFHH276 pKa = 7.36SEE278 pKa = 2.61QWYY281 pKa = 10.02NLRR284 pKa = 11.84SYY286 pKa = 11.18LLRR289 pKa = 11.84LIGLPPHH296 pKa = 7.12IPPNDD301 pKa = 4.74PIRR304 pKa = 11.84VPNRR308 pKa = 11.84VLMLALISRR317 pKa = 11.84LQDD320 pKa = 2.89VYY322 pKa = 11.13VAHH325 pKa = 6.58SPIDD329 pKa = 3.21IVAQQEE335 pKa = 4.61GNGQAGLPDD344 pKa = 3.83AALEE348 pKa = 3.95ALRR351 pKa = 11.84RR352 pKa = 11.84VV353 pKa = 3.65

Molecular weight: 39.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2Q1D6|Q2Q1D6_9REOV Non-structural protein NS2 OS=Peruvian horse sickness virus OX=356862 PE=4 SV=1
MM1 pKa = 6.65MHH3 pKa = 7.27AALSMTKK10 pKa = 9.91HH11 pKa = 6.0EE12 pKa = 4.62NPISLLTVVPATAPRR27 pKa = 11.84FDD29 pKa = 5.38EE30 pKa = 4.18ITDD33 pKa = 5.05LISSKK38 pKa = 10.73GEE40 pKa = 3.82KK41 pKa = 10.26EE42 pKa = 3.88EE43 pKa = 4.25VKK45 pKa = 9.39ITMSEE50 pKa = 3.93TMNAKK55 pKa = 6.99TTALEE60 pKa = 4.24VLSSALGNGSGTDD73 pKa = 3.21EE74 pKa = 3.68ITRR77 pKa = 11.84RR78 pKa = 11.84EE79 pKa = 3.79RR80 pKa = 11.84SAFGAATQALNEE92 pKa = 4.21EE93 pKa = 4.68EE94 pKa = 3.97NTRR97 pKa = 11.84RR98 pKa = 11.84LKK100 pKa = 11.06VYY102 pKa = 9.6MNEE105 pKa = 4.09QILPKK110 pKa = 10.38LKK112 pKa = 10.44NKK114 pKa = 10.23LMTVTYY120 pKa = 10.11KK121 pKa = 10.54YY122 pKa = 10.39RR123 pKa = 11.84IWLAVEE129 pKa = 4.52IILAIFSMVVFGVMAVNEE147 pKa = 4.39AEE149 pKa = 4.26SEE151 pKa = 4.26INSWLQNKK159 pKa = 10.01LGVKK163 pKa = 9.91VGLSAVSLGCTFALLFASRR182 pKa = 11.84SAGALKK188 pKa = 9.39EE189 pKa = 4.14TVKK192 pKa = 10.53RR193 pKa = 11.84MKK195 pKa = 10.61RR196 pKa = 11.84EE197 pKa = 3.4ITKK200 pKa = 10.29RR201 pKa = 11.84EE202 pKa = 4.14TYY204 pKa = 10.46NHH206 pKa = 6.77IAGTMAAGTEE216 pKa = 3.78RR217 pKa = 11.84RR218 pKa = 11.84VEE220 pKa = 3.97NSEE223 pKa = 4.14SRR225 pKa = 11.84VSNHH229 pKa = 6.48LNAAWINAPGTTNDD243 pKa = 3.16VPGWQLVRR251 pKa = 11.84LKK253 pKa = 10.82EE254 pKa = 4.13GG255 pKa = 3.17

Molecular weight: 28.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6223

255

1311

622.3

71.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.448 ± 0.543

1.302 ± 0.174

5.335 ± 0.44

7.312 ± 0.529

4.162 ± 0.326

5.399 ± 0.307

2.105 ± 0.233

7.167 ± 0.374

6.701 ± 0.812

9.417 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.166 ± 0.24

4.853 ± 0.173

3.664 ± 0.499

3.471 ± 0.293

6.299 ± 0.198

7.263 ± 0.313

5.962 ± 0.36

6.492 ± 0.385

1.093 ± 0.241

3.391 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski