Citromicrobium phage vB_Cib_ssDNA_P1

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PJ67|A0A2H4PJ67_9VIRU Replication initiator OS=Citromicrobium phage vB_Cib_ssDNA_P1 OX=2053684 PE=4 SV=1
MM1 pKa = 7.29EE2 pKa = 5.9NIMRR6 pKa = 11.84TLMSAPPTGRR16 pKa = 11.84VGRR19 pKa = 11.84YY20 pKa = 6.92PKK22 pKa = 10.55HH23 pKa = 6.4PFLTQEE29 pKa = 4.55KK30 pKa = 9.31PFTAQPFFLARR41 pKa = 11.84VLPGEE46 pKa = 4.11TLQNLRR52 pKa = 11.84LEE54 pKa = 4.5SRR56 pKa = 11.84VITDD60 pKa = 4.82PIKK63 pKa = 10.91NSIIGWSKK71 pKa = 9.69EE72 pKa = 3.73YY73 pKa = 10.58YY74 pKa = 8.92VFYY77 pKa = 11.25VKK79 pKa = 10.76VSDD82 pKa = 5.08LMIDD86 pKa = 4.11AIKK89 pKa = 11.15DD90 pKa = 3.46MFIDD94 pKa = 3.67PTNAEE99 pKa = 3.89ITGQDD104 pKa = 2.85AATNVQRR111 pKa = 11.84TYY113 pKa = 10.3TPQGGIDD120 pKa = 3.54WTGMCLTRR128 pKa = 11.84IVEE131 pKa = 4.41TYY133 pKa = 10.38FRR135 pKa = 11.84DD136 pKa = 3.7EE137 pKa = 4.45GEE139 pKa = 4.33TAASHH144 pKa = 6.52ALADD148 pKa = 4.14GTPMVQIRR156 pKa = 11.84QNSFLDD162 pKa = 4.03SITDD166 pKa = 3.31KK167 pKa = 11.77DD168 pKa = 4.48EE169 pKa = 4.43IPEE172 pKa = 4.17GAAISTATDD181 pKa = 3.13AGDD184 pKa = 4.34LDD186 pKa = 4.13RR187 pKa = 11.84LMDD190 pKa = 4.35AFEE193 pKa = 4.39MARR196 pKa = 11.84SLGMANMSYY205 pKa = 11.15EE206 pKa = 4.15DD207 pKa = 3.71FLRR210 pKa = 11.84GYY212 pKa = 10.16GINIPDD218 pKa = 3.87KK219 pKa = 11.55DD220 pKa = 3.62EE221 pKa = 5.22DD222 pKa = 3.89KK223 pKa = 11.31PEE225 pKa = 5.63LIDD228 pKa = 4.85RR229 pKa = 11.84WKK231 pKa = 10.74DD232 pKa = 2.94WQYY235 pKa = 11.31PSNTINPATGAPSSACSWVFKK256 pKa = 10.77NGSRR260 pKa = 11.84DD261 pKa = 3.31PKK263 pKa = 10.41FFKK266 pKa = 10.86EE267 pKa = 3.76PGFLVGISVTRR278 pKa = 11.84PKK280 pKa = 11.03VYY282 pKa = 9.65MGNLSGSAASFAKK295 pKa = 10.29RR296 pKa = 11.84AWDD299 pKa = 3.23WMPNYY304 pKa = 10.48LMAYY308 pKa = 9.12PEE310 pKa = 4.21AALKK314 pKa = 10.47QFGVDD319 pKa = 3.51AGPLGVRR326 pKa = 11.84TTDD329 pKa = 3.04TDD331 pKa = 3.99SYY333 pKa = 9.32WIDD336 pKa = 3.33MRR338 pKa = 11.84DD339 pKa = 3.22EE340 pKa = 4.42LLYY343 pKa = 11.04GDD345 pKa = 4.51QFQNMHH351 pKa = 6.61AFDD354 pKa = 4.18AAGATANTDD363 pKa = 2.79GSNNLFGLPLTDD375 pKa = 4.18FTWKK379 pKa = 10.56YY380 pKa = 7.48PTEE383 pKa = 3.88AMINSLFVQDD393 pKa = 4.96DD394 pKa = 3.86GSGRR398 pKa = 11.84IRR400 pKa = 11.84QDD402 pKa = 2.94GYY404 pKa = 11.21VGLSIKK410 pKa = 9.97GAQGASPVDD419 pKa = 3.54YY420 pKa = 10.93TMGNIAEE427 pKa = 4.38TT428 pKa = 3.81

Molecular weight: 47.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PJ67|A0A2H4PJ67_9VIRU Replication initiator OS=Citromicrobium phage vB_Cib_ssDNA_P1 OX=2053684 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84DD4 pKa = 3.67DD5 pKa = 3.78ARR7 pKa = 11.84DD8 pKa = 3.74YY9 pKa = 11.44LWRR12 pKa = 11.84QQLGDD17 pKa = 3.5GHH19 pKa = 6.12NCSRR23 pKa = 11.84PVEE26 pKa = 4.18LPSIGLPSMLEE37 pKa = 3.87PDD39 pKa = 3.87RR40 pKa = 11.84TPLPVTIMARR50 pKa = 11.84CRR52 pKa = 11.84KK53 pKa = 9.81CPEE56 pKa = 4.04CLAHH60 pKa = 7.79RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84LWTARR68 pKa = 11.84AVDD71 pKa = 4.0EE72 pKa = 4.47VQASSRR78 pKa = 11.84TWFGTLTVAPAHH90 pKa = 6.3RR91 pKa = 11.84VRR93 pKa = 11.84LAYY96 pKa = 10.09RR97 pKa = 11.84AEE99 pKa = 4.09AKK101 pKa = 10.25VLRR104 pKa = 11.84AGGEE108 pKa = 4.34SLSSLDD114 pKa = 2.86RR115 pKa = 11.84SEE117 pKa = 4.81RR118 pKa = 11.84YY119 pKa = 9.5RR120 pKa = 11.84ILANEE125 pKa = 4.9LGQEE129 pKa = 3.96VTKK132 pKa = 9.77WLKK135 pKa = 10.17RR136 pKa = 11.84VRR138 pKa = 11.84KK139 pKa = 9.56QSGASLRR146 pKa = 11.84YY147 pKa = 9.44LLVAEE152 pKa = 4.37AHH154 pKa = 6.5KK155 pKa = 11.22SGDD158 pKa = 3.35PHH160 pKa = 5.22MHH162 pKa = 6.38ILVHH166 pKa = 6.68EE167 pKa = 5.36DD168 pKa = 3.16GDD170 pKa = 4.28PVTKK174 pKa = 10.54RR175 pKa = 11.84MLQDD179 pKa = 2.61QWRR182 pKa = 11.84IGFSQWKK189 pKa = 9.79LVDD192 pKa = 3.75QDD194 pKa = 3.51PGAAVYY200 pKa = 9.38VCKK203 pKa = 10.78YY204 pKa = 9.0LAKK207 pKa = 10.46DD208 pKa = 2.76ALTRR212 pKa = 11.84VRR214 pKa = 11.84ASRR217 pKa = 11.84RR218 pKa = 11.84YY219 pKa = 8.68GQPQLVRR226 pKa = 11.84SLTEE230 pKa = 4.6RR231 pKa = 11.84IHH233 pKa = 6.92KK234 pKa = 9.62MRR236 pKa = 11.84DD237 pKa = 3.2AVHH240 pKa = 6.0EE241 pKa = 4.28ASSRR245 pKa = 11.84RR246 pKa = 11.84EE247 pKa = 3.54PDD249 pKa = 2.73

Molecular weight: 28.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1322

37

428

220.3

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.682 ± 1.248

1.286 ± 0.388

5.9 ± 1.157

5.446 ± 0.691

3.48 ± 0.95

7.867 ± 1.11

1.815 ± 0.584

5.144 ± 0.726

4.614 ± 0.719

7.564 ± 0.792

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.177 ± 0.451

3.48 ± 0.789

8.169 ± 1.548

3.177 ± 0.457

7.262 ± 1.566

5.673 ± 0.651

5.825 ± 0.697

5.144 ± 1.43

2.118 ± 0.32

3.177 ± 0.533
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski