Apis mellifera associated microvirus 34

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTD4|A0A3S8UTD4_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 34 OX=2494763 PE=4 SV=1
MM1 pKa = 7.23SEE3 pKa = 4.1KK4 pKa = 10.87VKK6 pKa = 10.17TPTKK10 pKa = 10.85AEE12 pKa = 4.0VQQWMQKK19 pKa = 10.71DD20 pKa = 3.06IATAVAFLNMLRR32 pKa = 11.84NDD34 pKa = 3.52QAMLDD39 pKa = 3.81ALADD43 pKa = 3.67VALEE47 pKa = 4.11RR48 pKa = 11.84IKK50 pKa = 10.93AQEE53 pKa = 3.97EE54 pKa = 4.65SKK56 pKa = 10.51QVQPEE61 pKa = 4.16LPLTNGG67 pKa = 3.25

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTE6|A0A3S8UTE6_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 34 OX=2494763 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.25CPSPNFVWPHH12 pKa = 6.3DD13 pKa = 3.77KK14 pKa = 10.91VIAVPCGKK22 pKa = 10.25CLVCLSNKK30 pKa = 9.12RR31 pKa = 11.84HH32 pKa = 6.0DD33 pKa = 3.28WSVRR37 pKa = 11.84LMQEE41 pKa = 4.01YY42 pKa = 9.92KK43 pKa = 10.92VSDD46 pKa = 3.28NAFFVTLTYY55 pKa = 10.43SDD57 pKa = 4.33KK58 pKa = 10.39YY59 pKa = 10.66LPSFGVSKK67 pKa = 10.73RR68 pKa = 11.84HH69 pKa = 5.37LQLFFKK75 pKa = 10.5RR76 pKa = 11.84VRR78 pKa = 11.84KK79 pKa = 8.59EE80 pKa = 3.87CNRR83 pKa = 11.84LRR85 pKa = 11.84YY86 pKa = 9.15YY87 pKa = 11.14AVGEE91 pKa = 4.16YY92 pKa = 10.61GSRR95 pKa = 11.84TGRR98 pKa = 11.84PHH100 pKa = 5.09YY101 pKa = 9.66HH102 pKa = 7.0AIMFNVDD109 pKa = 3.45PKK111 pKa = 11.33VVTDD115 pKa = 4.01CWTLFNKK122 pKa = 7.66VTNKK126 pKa = 10.06KK127 pKa = 9.64EE128 pKa = 4.32PIGLVHH134 pKa = 6.78FGKK137 pKa = 8.82VTEE140 pKa = 4.62ASVQYY145 pKa = 7.68TLKK148 pKa = 11.22YY149 pKa = 9.59IVQKK153 pKa = 11.37NEE155 pKa = 3.69FPKK158 pKa = 10.52EE159 pKa = 3.97LNPSFSLMSRR169 pKa = 11.84GYY171 pKa = 11.0GLGLNYY177 pKa = 10.52LSDD180 pKa = 5.19RR181 pKa = 11.84MLSWHH186 pKa = 7.01RR187 pKa = 11.84DD188 pKa = 3.23DD189 pKa = 5.51DD190 pKa = 3.97RR191 pKa = 11.84VYY193 pKa = 10.48MVVDD197 pKa = 4.1GEE199 pKa = 4.4KK200 pKa = 10.33KK201 pKa = 10.13RR202 pKa = 11.84LPRR205 pKa = 11.84YY206 pKa = 8.58YY207 pKa = 10.19KK208 pKa = 10.31EE209 pKa = 4.76KK210 pKa = 9.25IWPAIKK216 pKa = 10.48QEE218 pKa = 4.9DD219 pKa = 4.71YY220 pKa = 9.97PQGWARR226 pKa = 11.84WTWRR230 pKa = 11.84RR231 pKa = 11.84EE232 pKa = 3.72DD233 pKa = 3.97CFNKK237 pKa = 9.79ARR239 pKa = 11.84KK240 pKa = 8.2EE241 pKa = 3.93AEE243 pKa = 3.45EE244 pKa = 4.05AEE246 pKa = 4.37RR247 pKa = 11.84YY248 pKa = 9.28NVEE251 pKa = 4.03KK252 pKa = 10.8VRR254 pKa = 11.84SEE256 pKa = 4.63GYY258 pKa = 10.4SNPEE262 pKa = 3.5QVITEE267 pKa = 3.88MRR269 pKa = 11.84NAVVNRR275 pKa = 11.84IQSKK279 pKa = 9.19VAFTQKK285 pKa = 10.02VFF287 pKa = 3.35

Molecular weight: 34.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1317

67

551

263.4

30.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.669 ± 1.303

0.759 ± 0.403

5.695 ± 0.551

6.986 ± 1.173

4.556 ± 0.444

5.695 ± 0.54

1.898 ± 0.523

3.721 ± 0.308

7.137 ± 1.825

7.062 ± 0.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.797 ± 0.65

4.784 ± 0.474

4.784 ± 0.707

4.328 ± 1.344

5.923 ± 0.724

6.302 ± 0.856

5.239 ± 0.486

7.213 ± 0.722

2.202 ± 0.275

4.252 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski