Nitrospira defluvii

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8P992|D8P992_9BACT Uncharacterized protein OS=Nitrospira defluvii OX=330214 GN=NIDE2233 PE=4 SV=1
MM1 pKa = 7.42LVLIHH6 pKa = 6.49KK7 pKa = 8.67EE8 pKa = 4.11CGGPALDD15 pKa = 4.13EE16 pKa = 4.62SPVGEE21 pKa = 4.22VCAIPVSQFPFTCFTCLEE39 pKa = 5.03DD40 pKa = 4.69ILDD43 pKa = 3.95EE44 pKa = 4.62SEE46 pKa = 4.11VRR48 pKa = 11.84LTEE51 pKa = 4.39DD52 pKa = 4.13LGII55 pKa = 4.48

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3U4Q8|B3U4Q8_9BACT Exported protein OS=Nitrospira defluvii OX=330214 GN=NIDE1478 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.13QAIRR7 pKa = 11.84SVLAGVPMGLQLGATGTGGAILGVPMMVYY36 pKa = 10.1IAGIPVRR43 pKa = 11.84QAAAMSLMIVATSSLLGAWEE63 pKa = 4.06YY64 pKa = 11.4GRR66 pKa = 11.84HH67 pKa = 5.38GLVKK71 pKa = 9.84PKK73 pKa = 10.41AAAAFSWTGMAGAWAGAFGHH93 pKa = 6.6HH94 pKa = 6.68LVPDD98 pKa = 4.14EE99 pKa = 4.25VLLICFGLLLLLTRR113 pKa = 11.84TLVSRR118 pKa = 11.84QRR120 pKa = 11.84ALMSQTEE127 pKa = 4.13PQEE130 pKa = 4.24GCATHH135 pKa = 7.22FPRR138 pKa = 11.84TCWLKK143 pKa = 10.18VGAIGLVVGTLNGVFGVGGGFLVVPALSVVLGFPTRR179 pKa = 11.84VAIGTSLTIISLISLSGVIGHH200 pKa = 6.66LQFGRR205 pKa = 11.84IDD207 pKa = 3.26WPLTGLVLVGSLSGMLVGVRR227 pKa = 11.84VGALLSPTTMGRR239 pKa = 11.84ITATVTVTIAMILIVVNVAKK259 pKa = 10.61LAGFQIVRR267 pKa = 3.81

Molecular weight: 27.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4263

0

4263

1285082

17

3120

301.5

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.122 ± 0.043

1.024 ± 0.014

5.119 ± 0.026

5.962 ± 0.038

3.562 ± 0.022

7.903 ± 0.033

2.386 ± 0.02

4.942 ± 0.026

3.786 ± 0.043

10.651 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.019

2.591 ± 0.023

5.218 ± 0.029

4.143 ± 0.027

7.127 ± 0.039

5.79 ± 0.033

5.758 ± 0.034

7.703 ± 0.035

1.337 ± 0.018

2.506 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski