Bacillus phage Slash

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Slashvirus; Bacillus virus Slash

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5Q0D3|U5Q0D3_9CAUD Uncharacterized protein OS=Bacillus phage Slash OX=1406790 GN=Slash_49 PE=4 SV=1
MM1 pKa = 7.04TRR3 pKa = 11.84FVKK6 pKa = 10.47ADD8 pKa = 3.44YY9 pKa = 11.31SNEE12 pKa = 3.78QDD14 pKa = 3.58FKK16 pKa = 11.17EE17 pKa = 4.46TFKK20 pKa = 11.51DD21 pKa = 3.33MSNFEE26 pKa = 3.85ITGIEE31 pKa = 4.22SYY33 pKa = 10.03IAEE36 pKa = 4.3WFEE39 pKa = 3.79EE40 pKa = 4.23MEE42 pKa = 4.61GYY44 pKa = 9.69EE45 pKa = 4.24VEE47 pKa = 4.33VSTAYY52 pKa = 10.56SSSTVPMLNVMNYY65 pKa = 8.98VEE67 pKa = 4.22YY68 pKa = 10.85EE69 pKa = 4.13GMEE72 pKa = 3.87VSYY75 pKa = 10.2FALVNAHH82 pKa = 5.65TLVMVLEE89 pKa = 4.56DD90 pKa = 3.4EE91 pKa = 4.8KK92 pKa = 11.53EE93 pKa = 4.13NNHH96 pKa = 6.06YY97 pKa = 10.97YY98 pKa = 10.57YY99 pKa = 10.16ISS101 pKa = 3.36

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5Q0G6|U5Q0G6_9CAUD Uncharacterized protein OS=Bacillus phage Slash OX=1406790 GN=Slash_84 PE=4 SV=1
MM1 pKa = 7.63ANHH4 pKa = 6.39YY5 pKa = 9.44KK6 pKa = 10.81NKK8 pKa = 9.08VRR10 pKa = 11.84RR11 pKa = 11.84NFIGNVKK18 pKa = 9.99VFMSDD23 pKa = 2.69HH24 pKa = 5.33AVIRR28 pKa = 11.84FVEE31 pKa = 4.22RR32 pKa = 11.84QNLDD36 pKa = 3.35PAGLDD41 pKa = 3.02INAVRR46 pKa = 11.84TEE48 pKa = 4.4IIRR51 pKa = 11.84KK52 pKa = 8.41FRR54 pKa = 11.84NSKK57 pKa = 10.65LGGFLANGKK66 pKa = 7.02EE67 pKa = 3.76RR68 pKa = 11.84RR69 pKa = 11.84YY70 pKa = 10.1EE71 pKa = 3.89VAGSMNEE78 pKa = 3.67RR79 pKa = 11.84MQFICKK85 pKa = 9.86KK86 pKa = 8.32EE87 pKa = 3.82EE88 pKa = 3.77CGKK91 pKa = 10.58YY92 pKa = 10.66VIISCQLQGKK102 pKa = 9.28RR103 pKa = 11.84NDD105 pKa = 2.61WWKK108 pKa = 11.37NEE110 pKa = 3.66GLVKK114 pKa = 10.67NAA116 pKa = 4.39

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

23879

25

2249

215.1

24.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.37 ± 0.46

0.884 ± 0.126

6.252 ± 0.182

7.479 ± 0.329

4.565 ± 0.21

6.303 ± 0.253

1.667 ± 0.125

6.495 ± 0.195

8.895 ± 0.263

7.634 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.045 ± 0.192

6.005 ± 0.195

2.705 ± 0.175

3.991 ± 0.303

3.991 ± 0.197

5.863 ± 0.227

6.089 ± 0.306

6.663 ± 0.199

1.06 ± 0.074

4.045 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski