Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Ideonella

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K8P260|A0A0K8P260_IDESA Tricarboxylate transport protein TctB OS=Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) OX=1547922 GN=ISF6_2484 PE=4 SV=1
MM1 pKa = 7.14NAEE4 pKa = 4.38DD5 pKa = 4.91FDD7 pKa = 5.08VEE9 pKa = 4.57LTCPTCSRR17 pKa = 11.84HH18 pKa = 4.67FQAGVTEE25 pKa = 4.39LLARR29 pKa = 11.84PIATCPCGQPVQVDD43 pKa = 3.64VAALRR48 pKa = 11.84EE49 pKa = 4.2SLGLDD54 pKa = 3.18EE55 pKa = 6.27GDD57 pKa = 3.38

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K8NYC9|A0A0K8NYC9_IDESA Putative exported protein OS=Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) OX=1547922 GN=ISF6_1149 PE=4 SV=1
MM1 pKa = 7.16SQPPGRR7 pKa = 11.84RR8 pKa = 11.84PARR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84LAGPPAPGRR22 pKa = 11.84APARR26 pKa = 11.84ASPPGGRR33 pKa = 11.84RR34 pKa = 11.84GTMRR38 pKa = 11.84IGPAAGLARR47 pKa = 11.84PP48 pKa = 4.06

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5527

0

5527

1811574

37

3234

327.8

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.184 ± 0.061

0.894 ± 0.011

5.322 ± 0.022

5.12 ± 0.023

3.052 ± 0.022

9.028 ± 0.031

2.195 ± 0.018

3.314 ± 0.026

2.107 ± 0.034

11.182 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.016

1.873 ± 0.021

6.368 ± 0.037

3.566 ± 0.021

8.603 ± 0.043

4.538 ± 0.024

4.605 ± 0.026

7.631 ± 0.027

1.545 ± 0.016

1.865 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski