Sulfobacillus thermosulfidooxidans (strain DSM 9293 / VKM B-1269 / AT-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Clostridiales Family XVII. Incertae Sedis; Sulfobacillus; Sulfobacillus thermosulfidooxidans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1WPB6|A0A1W1WPB6_SULTA Uncharacterized protein OS=Sulfobacillus thermosulfidooxidans (strain DSM 9293 / VKM B-1269 / AT-1) OX=929705 GN=SAMN00768000_3617 PE=4 SV=1
MM1 pKa = 7.94PPRR4 pKa = 11.84LAPVTNLAAFWDD16 pKa = 4.31ADD18 pKa = 4.06PPPLSPAVTLIARR31 pKa = 11.84FLQEE35 pKa = 3.79DD36 pKa = 5.33TITDD40 pKa = 2.87WHH42 pKa = 6.63TFTFSDD48 pKa = 3.6VQAAYY53 pKa = 9.74IDD55 pKa = 3.98LLFYY59 pKa = 10.66TPIEE63 pKa = 5.01HH64 pKa = 7.32IDD66 pKa = 4.09DD67 pKa = 4.44YY68 pKa = 11.88DD69 pKa = 5.82DD70 pKa = 5.42EE71 pKa = 7.47DD72 pKa = 6.11DD73 pKa = 4.98GDD75 pKa = 4.05VNRR78 pKa = 11.84DD79 pKa = 3.95NLWHH83 pKa = 7.18PLLQVSTLIDD93 pKa = 3.7TLAMEE98 pKa = 5.58PGFCPPTIPFYY109 pKa = 11.32

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1WD54|A0A1W1WD54_SULTA Thioredoxin reductase (NADPH) OS=Sulfobacillus thermosulfidooxidans (strain DSM 9293 / VKM B-1269 / AT-1) OX=929705 GN=SAMN00768000_1544 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.25QPNRR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.7RR12 pKa = 11.84AKK14 pKa = 10.32VHH16 pKa = 5.33GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 9.94AGRR28 pKa = 11.84MVLKK32 pKa = 10.41RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.36GRR39 pKa = 11.84KK40 pKa = 8.83RR41 pKa = 11.84LAGG44 pKa = 3.61

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1062604

30

1531

293.9

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.907 ± 0.044

0.647 ± 0.011

4.845 ± 0.034

5.199 ± 0.045

3.684 ± 0.028

7.416 ± 0.037

3.122 ± 0.025

6.208 ± 0.035

3.322 ± 0.029

10.773 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.022

2.944 ± 0.028

5.468 ± 0.032

4.646 ± 0.03

5.888 ± 0.041

5.947 ± 0.037

5.683 ± 0.032

7.677 ± 0.033

2.096 ± 0.026

2.907 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski