Geobacillus phage TP-84

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saundersvirus; Geobacillus virus Tp84

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9WQQ6|A0A1U9WQQ6_9CAUD Uncharacterized protein OS=Geobacillus phage TP-84 OX=1965361 PE=4 SV=1
MM1 pKa = 7.08VKK3 pKa = 10.28CAICGYY9 pKa = 9.94EE10 pKa = 3.77LTIDD14 pKa = 3.76EE15 pKa = 5.33EE16 pKa = 4.81IEE18 pKa = 4.35VMLADD23 pKa = 5.62DD24 pKa = 5.69DD25 pKa = 4.66EE26 pKa = 4.91CPKK29 pKa = 10.83CGGGLTVEE37 pKa = 4.46

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1Q718|A0A2D1Q718_9CAUD Putative membrane protein OS=Geobacillus phage TP-84 OX=1965361 PE=4 SV=1
MM1 pKa = 7.7WIDD4 pKa = 3.34GNEE7 pKa = 3.62WRR9 pKa = 11.84TNAARR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84KK18 pKa = 8.5RR19 pKa = 11.84SKK21 pKa = 10.74RR22 pKa = 11.84KK23 pKa = 9.23GGCRR27 pKa = 11.84MGGKK31 pKa = 9.04PSKK34 pKa = 9.76GTPKK38 pKa = 10.87DD39 pKa = 3.25MRR41 pKa = 11.84LKK43 pKa = 10.5RR44 pKa = 11.84NRR46 pKa = 11.84KK47 pKa = 8.64AAGLPVGPGSRR58 pKa = 11.84SKK60 pKa = 10.04KK61 pKa = 6.15TTSKK65 pKa = 10.78RR66 pKa = 11.84KK67 pKa = 9.38

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14285

37

991

180.8

20.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.51 ± 0.453

0.7 ± 0.118

6.363 ± 0.357

7.21 ± 0.412

3.647 ± 0.166

7.056 ± 0.264

2.058 ± 0.179

7.784 ± 0.339

8.148 ± 0.436

6.517 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.584 ± 0.198

4.655 ± 0.303

3.934 ± 0.229

4.767 ± 0.279

4.991 ± 0.305

4.298 ± 0.225

6.041 ± 0.259

6.069 ± 0.264

1.736 ± 0.116

3.927 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski