EBPR podovirus 2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8TUT2|F8TUT2_9CAUD Uncharacterized protein gp33 OS=EBPR podovirus 2 OX=1048516 GN=gp33 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 2.72HH4 pKa = 6.64GFYY7 pKa = 10.65HH8 pKa = 6.9PEE10 pKa = 3.24IGYY13 pKa = 8.16WQTNADD19 pKa = 3.84VPDD22 pKa = 6.0DD23 pKa = 3.6ILAGYY28 pKa = 9.13PEE30 pKa = 4.5GTVEE34 pKa = 4.31VPLMPEE40 pKa = 5.11AGATWDD46 pKa = 3.73GQQWQPATPPLPSAEE61 pKa = 4.48DD62 pKa = 3.37YY63 pKa = 10.92RR64 pKa = 11.84LAVQAHH70 pKa = 5.44VDD72 pKa = 3.58AVAVARR78 pKa = 11.84LYY80 pKa = 11.21DD81 pKa = 3.75SGVSLASYY89 pKa = 9.43VASTNATWAAEE100 pKa = 3.92AAVFVAWRR108 pKa = 11.84DD109 pKa = 3.62AVWAQVYY116 pKa = 8.22GLWAAPPDD124 pKa = 4.45PVPLPAEE131 pKa = 4.43VVAGLPVIEE140 pKa = 4.23WPGVGEE146 pKa = 4.06

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8TUU7|F8TUU7_9CAUD Uncharacterized protein gp48 OS=EBPR podovirus 2 OX=1048516 GN=gp48 PE=4 SV=1
MM1 pKa = 6.84TRR3 pKa = 11.84AEE5 pKa = 3.95IRR7 pKa = 11.84IAAAKK12 pKa = 9.87KK13 pKa = 9.57RR14 pKa = 11.84ATAAEE19 pKa = 4.4AKK21 pKa = 9.81WSDD24 pKa = 2.89ALAAKK29 pKa = 10.35RR30 pKa = 11.84EE31 pKa = 4.29ATWRR35 pKa = 11.84IKK37 pKa = 10.2AAHH40 pKa = 5.99NQRR43 pKa = 11.84IWPLRR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 9.16MDD52 pKa = 3.84EE53 pKa = 3.98AYY55 pKa = 9.8MAVAVAEE62 pKa = 4.09LAAMGINPMKK72 pKa = 10.31TIILWHH78 pKa = 5.96PRR80 pKa = 11.84GYY82 pKa = 8.52TAPSAQNRR90 pKa = 11.84YY91 pKa = 6.49VVRR94 pKa = 11.84VTRR97 pKa = 11.84EE98 pKa = 3.57GWKK101 pKa = 10.61LLVPVGKK108 pKa = 8.87TGVILRR114 pKa = 11.84NRR116 pKa = 11.84NDD118 pKa = 3.11QSGPSRR124 pKa = 11.84WDD126 pKa = 3.0KK127 pKa = 10.65VTVTDD132 pKa = 5.46QEE134 pKa = 4.47VQPP137 pKa = 4.56

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12610

49

702

188.2

20.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.18 ± 0.474

1.007 ± 0.139

6.487 ± 0.278

5.813 ± 0.289

2.847 ± 0.16

8.795 ± 0.51

1.784 ± 0.197

4.433 ± 0.148

3.878 ± 0.309

7.526 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.18

2.998 ± 0.229

5.4 ± 0.288

3.886 ± 0.317

7.415 ± 0.385

5.377 ± 0.253

5.884 ± 0.41

6.122 ± 0.286

1.911 ± 0.155

2.363 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski