Candidatus Vecturithrix granuli

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Candidatus Vecturithrix

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081BUC5|A0A081BUC5_9BACT Multicomponent Na+:H+ antiporter subunit B OS=Candidatus Vecturithrix granuli OX=1499967 GN=U27_02891 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.33RR3 pKa = 11.84QVNRR7 pKa = 11.84QLLYY11 pKa = 9.05MHH13 pKa = 7.34IFSKK17 pKa = 10.02TISFVLLLGIVGAVLIGIGGCKK39 pKa = 9.27GVNVNPTTPTIDD51 pKa = 3.39TPTPEE56 pKa = 4.78PEE58 pKa = 4.14STATPDD64 pKa = 3.43GGEE67 pKa = 4.12STPTPEE73 pKa = 6.16GEE75 pKa = 4.16GCDD78 pKa = 3.94NYY80 pKa = 11.44GEE82 pKa = 4.61TPDD85 pKa = 4.46DD86 pKa = 4.24PNLIVLMPDD95 pKa = 3.56GDD97 pKa = 4.04PVAISMDD104 pKa = 3.86CTDD107 pKa = 5.31DD108 pKa = 3.58IDD110 pKa = 3.59WYY112 pKa = 10.69KK113 pKa = 10.6IEE115 pKa = 4.18ITQSPVTLEE124 pKa = 3.77IKK126 pKa = 9.37LTNLPGNSDD135 pKa = 3.99YY136 pKa = 11.82DD137 pKa = 4.44LIAYY141 pKa = 7.93DD142 pKa = 3.96ANLLEE147 pKa = 4.37MEE149 pKa = 4.87NGRR152 pKa = 11.84SAQTGNVDD160 pKa = 2.81EE161 pKa = 4.58HH162 pKa = 6.98LRR164 pKa = 11.84LNVTDD169 pKa = 4.59FMIYY173 pKa = 10.31LQIHH177 pKa = 6.43SYY179 pKa = 9.65NGKK182 pKa = 7.94GTAILRR188 pKa = 11.84ISIVDD193 pKa = 3.83DD194 pKa = 4.17SPVEE198 pKa = 4.27PGDD201 pKa = 5.55DD202 pKa = 5.02DD203 pKa = 6.88DD204 pKa = 7.59GDD206 pKa = 5.58DD207 pKa = 4.62DD208 pKa = 6.54DD209 pKa = 6.93GDD211 pKa = 3.83EE212 pKa = 4.75FEE214 pKa = 4.27QLSYY218 pKa = 11.57EE219 pKa = 4.64DD220 pKa = 4.79ILFQEE225 pKa = 4.87LSSYY229 pKa = 10.6PRR231 pKa = 11.84GTMSSLLEE239 pKa = 3.94RR240 pKa = 11.84SVEE243 pKa = 4.12TTITKK248 pKa = 10.2SVFCQLTDD256 pKa = 3.39SQLTGTFTIGNYY268 pKa = 10.06AHH270 pKa = 6.55VEE272 pKa = 3.84RR273 pKa = 11.84DD274 pKa = 3.43FLQSVDD280 pKa = 5.06YY281 pKa = 10.75IDD283 pKa = 4.04GWSLVVFNGSKK294 pKa = 10.51EE295 pKa = 4.03LLDD298 pKa = 4.07NLTLTIRR305 pKa = 11.84VTLYY309 pKa = 10.83APDD312 pKa = 3.38VGLEE316 pKa = 3.96VEE318 pKa = 4.54VPYY321 pKa = 11.44NMEE324 pKa = 3.95IDD326 pKa = 3.82GNDD329 pKa = 3.51YY330 pKa = 9.46LTSTSEE336 pKa = 3.58NDD338 pKa = 3.22VYY340 pKa = 11.53YY341 pKa = 10.99LSQKK345 pKa = 10.83YY346 pKa = 10.35GDD348 pKa = 3.82LAGNSSSDD356 pKa = 3.03IRR358 pKa = 11.84ITSGVVGDD366 pKa = 4.75IGDD369 pKa = 4.38LFANNIFTQQVEE381 pKa = 4.61VSWEE385 pKa = 3.97YY386 pKa = 9.95TDD388 pKa = 4.86EE389 pKa = 4.57SGGYY393 pKa = 9.76CSGRR397 pKa = 11.84VSGRR401 pKa = 11.84SIVDD405 pKa = 3.84FAALSIRR412 pKa = 3.82

Molecular weight: 45.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081BXK5|A0A081BXK5_9BACT HEPN_Swt1 domain-containing protein OS=Candidatus Vecturithrix granuli OX=1499967 GN=U27_04024 PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 4.6TVDD5 pKa = 3.85EE6 pKa = 4.4RR7 pKa = 11.84TGSSRR12 pKa = 11.84SRR14 pKa = 11.84QTVWPSLRR22 pKa = 11.84IPQASRR28 pKa = 11.84DD29 pKa = 3.8WASTTAAYY37 pKa = 9.78RR38 pKa = 11.84LCANPKK44 pKa = 9.27VSAARR49 pKa = 11.84ILEE52 pKa = 3.84PHH54 pKa = 5.76QRR56 pKa = 11.84QTCRR60 pKa = 11.84RR61 pKa = 11.84MAGEE65 pKa = 4.05SRR67 pKa = 11.84ILAGQEE73 pKa = 4.04TCFLNYY79 pKa = 9.16TSHH82 pKa = 7.11PTTEE86 pKa = 4.05TGQMRR91 pKa = 11.84LVLRR95 pKa = 11.84ARR97 pKa = 11.84PVSGYY102 pKa = 7.71VTVAIPAQPQRR113 pKa = 11.84PARR116 pKa = 11.84TATVEE121 pKa = 3.77VRR123 pKa = 11.84YY124 pKa = 10.75GEE126 pKa = 4.29VTLRR130 pKa = 11.84PPSRR134 pKa = 11.84DD135 pKa = 3.29PSCQADD141 pKa = 4.13LRR143 pKa = 11.84PLTLAVVWARR153 pKa = 11.84EE154 pKa = 4.11VEE156 pKa = 4.07ASGDD160 pKa = 3.59VATPLEE166 pKa = 4.5WLLMTTVSIQTFLDD180 pKa = 3.2AVEE183 pKa = 3.92RR184 pKa = 11.84MRR186 pKa = 11.84WYY188 pKa = 10.3RR189 pKa = 11.84LRR191 pKa = 11.84WHH193 pKa = 6.88IEE195 pKa = 3.51IFHH198 pKa = 6.63KK199 pKa = 10.51VLKK202 pKa = 9.64SGCRR206 pKa = 11.84IADD209 pKa = 3.41YY210 pKa = 11.05RR211 pKa = 11.84LATVDD216 pKa = 3.37KK217 pKa = 10.19LVRR220 pKa = 11.84SVTLKK225 pKa = 10.97SVVAWQMQWMGPLHH239 pKa = 5.96RR240 pKa = 11.84TSTLPEE246 pKa = 4.09AVPTVHH252 pKa = 6.84QMVRR256 pKa = 11.84WIAKK260 pKa = 9.72LGGFLGRR267 pKa = 11.84KK268 pKa = 8.77HH269 pKa = 6.62DD270 pKa = 4.4RR271 pKa = 11.84EE272 pKa = 4.17PGGAGNGFTISPPCGTCCMSPHH294 pKa = 4.94LHH296 pKa = 6.35KK297 pKa = 10.87RR298 pKa = 11.84GGYY301 pKa = 8.55PGGRR305 pKa = 11.84NTGRR309 pKa = 11.84SRR311 pKa = 11.84GRR313 pKa = 11.84VCFSVEE319 pKa = 3.6QYY321 pKa = 10.13VRR323 pKa = 11.84IMIIRR328 pKa = 3.91

Molecular weight: 36.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7048

0

7048

2347583

29

2850

333.1

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.031 ± 0.027

1.103 ± 0.011

4.922 ± 0.022

6.774 ± 0.034

4.553 ± 0.023

6.635 ± 0.028

2.357 ± 0.014

7.188 ± 0.028

5.041 ± 0.029

10.513 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.014

3.523 ± 0.016

4.353 ± 0.019

4.792 ± 0.026

5.186 ± 0.021

5.546 ± 0.021

5.574 ± 0.022

6.713 ± 0.024

1.235 ± 0.012

3.469 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski