Pararhizobium antarcticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Pararhizobium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A657LPZ9|A0A657LPZ9_9RHIZ Chaperone protein DnaK OS=Pararhizobium antarcticum OX=1798805 GN=dnaK PE=2 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGANAADD24 pKa = 4.6AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.83TCLKK58 pKa = 9.4IGGYY62 pKa = 9.79VRR64 pKa = 11.84TQINYY69 pKa = 10.57DD70 pKa = 3.18EE71 pKa = 5.15DD72 pKa = 3.97LTVNGADD79 pKa = 2.89WDD81 pKa = 3.88ARR83 pKa = 11.84TRR85 pKa = 11.84GYY87 pKa = 9.51LTFAAKK93 pKa = 10.46NDD95 pKa = 3.91TEE97 pKa = 4.23YY98 pKa = 9.71GTLSGYY104 pKa = 10.48INLQADD110 pKa = 3.85DD111 pKa = 4.34TNDD114 pKa = 3.46TFLDD118 pKa = 4.36GAWINIAGFDD128 pKa = 3.48VGYY131 pKa = 10.12FYY133 pKa = 11.44NWWDD137 pKa = 3.63DD138 pKa = 3.39MGLSGEE144 pKa = 4.35TDD146 pKa = 3.13VSAGNLSNAIRR157 pKa = 11.84YY158 pKa = 6.62TYY160 pKa = 10.82DD161 pKa = 3.01GGTFKK166 pKa = 11.14VGVAAEE172 pKa = 3.99EE173 pKa = 4.06LAGNGTNDD181 pKa = 3.62DD182 pKa = 4.08VGVSGYY188 pKa = 10.15VAGSIGGVTADD199 pKa = 4.66LVASYY204 pKa = 11.12DD205 pKa = 3.56FDD207 pKa = 4.03VEE209 pKa = 4.12QFAVKK214 pKa = 10.11GRR216 pKa = 11.84LVAEE220 pKa = 4.87IGPGALGILGVYY232 pKa = 10.44ADD234 pKa = 4.18GANFFWDD241 pKa = 3.48VSEE244 pKa = 4.04WSVAAEE250 pKa = 4.01YY251 pKa = 10.28KK252 pKa = 10.33IQATEE257 pKa = 3.96KK258 pKa = 10.8FFITPAAQYY267 pKa = 10.06FGDD270 pKa = 3.81FAFVDD275 pKa = 5.16GNDD278 pKa = 3.03AWKK281 pKa = 10.71VGVTAGYY288 pKa = 10.33QITEE292 pKa = 4.19GLRR295 pKa = 11.84TLATVNYY302 pKa = 10.13LDD304 pKa = 3.91VDD306 pKa = 3.92NVADD310 pKa = 4.24GEE312 pKa = 4.47WTGFVRR318 pKa = 11.84LQRR321 pKa = 11.84DD322 pKa = 3.62FF323 pKa = 4.22

Molecular weight:
34.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A657LKY1|A0A657LKY1_9RHIZ Conjugal transfer protein TrbE OS=Pararhizobium antarcticum OX=1798805 GN=AX760_24775 PE=3 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 8.95RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.81GFRR20 pKa = 11.84SRR22 pKa = 11.84IATKK26 pKa = 10.34GGRR29 pKa = 11.84KK30 pKa = 9.47VITARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight:
5.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4832

0

4832

1519063

41

2833

314.4

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.902 ± 0.047

0.834 ± 0.009

5.885 ± 0.028

5.5 ± 0.029

3.947 ± 0.025

8.352 ± 0.033

2.027 ± 0.016

5.808 ± 0.028

3.652 ± 0.029

9.959 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.016

2.804 ± 0.019

4.775 ± 0.025

3.14 ± 0.02

6.526 ± 0.033

5.794 ± 0.028

5.593 ± 0.022

7.279 ± 0.027

1.269 ± 0.013

2.261 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski