Clostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lacrimispora; Lacrimispora saccharolytica

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9RA59|D9RA59_CLOSW Uncharacterized protein OS=Clostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1) OX=610130 GN=Closa_3506 PE=4 SV=1
MM1 pKa = 7.46NKK3 pKa = 9.88RR4 pKa = 11.84LFSMFLALCMIVTMLPVSAMAEE26 pKa = 4.26EE27 pKa = 4.05IHH29 pKa = 5.41TTIGGSGEE37 pKa = 4.23IISFAPLTEE46 pKa = 3.91TAKK49 pKa = 10.73AVSLGTSIEE58 pKa = 4.06DD59 pKa = 3.61LEE61 pKa = 4.78LPEE64 pKa = 4.5TLTATVRR71 pKa = 11.84TAVPADD77 pKa = 3.72EE78 pKa = 6.49DD79 pKa = 3.99STQDD83 pKa = 3.12SGSPEE88 pKa = 4.04TATPTTAAEE97 pKa = 4.45PKK99 pKa = 9.12WKK101 pKa = 8.4EE102 pKa = 3.99TTGDD106 pKa = 3.89IPVEE110 pKa = 4.1WASPDD115 pKa = 3.27YY116 pKa = 11.79DD117 pKa = 3.45MDD119 pKa = 4.07TEE121 pKa = 4.41GVYY124 pKa = 10.7VFTPVIEE131 pKa = 5.12GYY133 pKa = 8.18TVSAPLPEE141 pKa = 4.14LTVTVGEE148 pKa = 4.49MPPIAAARR156 pKa = 11.84GEE158 pKa = 4.2VALLSEE164 pKa = 5.36TIPEE168 pKa = 4.12LWVGGVQVTSANASDD183 pKa = 3.92VLGEE187 pKa = 4.05ADD189 pKa = 3.73GDD191 pKa = 4.35GATVIYY197 pKa = 10.41DD198 pKa = 3.83PASGTLTLDD207 pKa = 3.33NANITNGYY215 pKa = 7.46NTTIDD220 pKa = 3.64DD221 pKa = 3.93RR222 pKa = 11.84NVQFGICSTLNALKK236 pKa = 10.63LVVVGTNTITCPNASVKK253 pKa = 10.44DD254 pKa = 3.6SYY256 pKa = 11.68AIYY259 pKa = 8.15TTGTLEE265 pKa = 3.83ISEE268 pKa = 4.58SGSGNLTATGGTASDD283 pKa = 4.01ISAGMYY289 pKa = 10.36VSDD292 pKa = 4.3GLTIEE297 pKa = 4.68SGSLTATGGTAIYY310 pKa = 9.31LQSKK314 pKa = 9.98SYY316 pKa = 10.71GVYY319 pKa = 10.49ADD321 pKa = 3.73SLTIEE326 pKa = 4.76GGSLTANGGAVTYY339 pKa = 10.26QSGEE343 pKa = 4.15SYY345 pKa = 10.74GAYY348 pKa = 10.06ADD350 pKa = 3.84SLTIEE355 pKa = 4.41SGSLIAKK362 pKa = 9.72GGAATGTFGKK372 pKa = 10.41SYY374 pKa = 10.66GVYY377 pKa = 10.04AGNSVSVSNDD387 pKa = 2.6GTLTATGGMASNSGTSAGVYY407 pKa = 10.17LNNGDD412 pKa = 3.89STDD415 pKa = 3.8SNGSVSVSDD424 pKa = 4.13GGILTATGGMASTTSVGVYY443 pKa = 9.92VNNISSNGSTVSVSVSDD460 pKa = 5.1DD461 pKa = 3.8GEE463 pKa = 4.21LTAKK467 pKa = 10.53GGTASGTFGTSVGVYY482 pKa = 9.63FDD484 pKa = 4.42NRR486 pKa = 11.84SSTDD490 pKa = 3.04STGSVSVSGSGKK502 pKa = 8.49LTATGDD508 pKa = 3.65AASGTFGTSAGVYY521 pKa = 9.57FDD523 pKa = 4.53NRR525 pKa = 11.84SSTGSTGSVSVSDD538 pKa = 4.41DD539 pKa = 3.47GKK541 pKa = 9.92LTATGDD547 pKa = 3.77AASGSQGTSAGVYY560 pKa = 9.85VNNNSTDD567 pKa = 3.1STGRR571 pKa = 11.84VSVSGHH577 pKa = 6.19GEE579 pKa = 3.76LTATGGMASATSAGVFSNGSGSSVSVSDD607 pKa = 5.07DD608 pKa = 3.25GSLTATGNAASASYY622 pKa = 10.42GVSAEE627 pKa = 4.13NGSMSIFDD635 pKa = 3.88GTITAQSGAASSTSAGVYY653 pKa = 10.04SGSLSVGGGMVTATGGTASDD673 pKa = 3.7ISGKK677 pKa = 9.97SAGVYY682 pKa = 7.41ATSVSVFGSGEE693 pKa = 3.83LTATGDD699 pKa = 3.68AASEE703 pKa = 4.46TNGTSAGVFSNGSGSSVSVSDD724 pKa = 5.07DD725 pKa = 3.25GSLTATGDD733 pKa = 3.58EE734 pKa = 4.04ASGFVGTSAGVYY746 pKa = 7.81FQSSNGSMTVSGGTVEE762 pKa = 4.28ATGGTASAISGGVYY776 pKa = 9.84FQYY779 pKa = 10.33SGSMTISGGTVTAQSGTAATSQAMNTPPPRR809 pKa = 11.84PP810 pKa = 3.44

Molecular weight: 79.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9R425|D9R425_CLOSW Amino acid carrier protein OS=Clostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1) OX=610130 GN=Closa_0501 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4133

0

4133

1343575

30

5128

325.1

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.507 ± 0.04

1.39 ± 0.02

5.356 ± 0.027

7.326 ± 0.046

4.15 ± 0.031

7.569 ± 0.045

1.729 ± 0.017

7.299 ± 0.037

6.531 ± 0.029

9.158 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.167 ± 0.022

4.277 ± 0.023

3.546 ± 0.03

3.227 ± 0.023

4.483 ± 0.032

6.117 ± 0.033

5.415 ± 0.042

6.778 ± 0.028

0.946 ± 0.014

4.029 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski