Avon-Heathcote Estuary associated circular virus 21

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMK1|A0A0C5IMK1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 21 OX=1618245 PE=4 SV=1
MM1 pKa = 7.56PKK3 pKa = 10.09ARR5 pKa = 11.84PSNGSRR11 pKa = 11.84FWCFTINNPTDD22 pKa = 4.3DD23 pKa = 5.79DD24 pKa = 4.16NQQLADD30 pKa = 4.15LMSNDD35 pKa = 3.39LCTYY39 pKa = 9.55LCYY42 pKa = 10.55GRR44 pKa = 11.84EE45 pKa = 4.18TGEE48 pKa = 4.29SEE50 pKa = 4.25TPHH53 pKa = 4.99YY54 pKa = 9.51QAYY57 pKa = 9.83IEE59 pKa = 4.61LLVPNDD65 pKa = 3.58FLGLRR70 pKa = 11.84NDD72 pKa = 3.7LKK74 pKa = 11.17EE75 pKa = 5.02LILNHH80 pKa = 6.58ASEE83 pKa = 4.33AAPRR87 pKa = 11.84QEE89 pKa = 3.87TTASRR94 pKa = 11.84KK95 pKa = 9.5ISTQSNMVSGSPIAKK110 pKa = 9.39GKK112 pKa = 10.79GMTSRR117 pKa = 11.84FFTEE121 pKa = 3.85YY122 pKa = 10.58RR123 pKa = 11.84AMHH126 pKa = 6.03AMEE129 pKa = 5.48RR130 pKa = 11.84DD131 pKa = 3.74APTKK135 pKa = 10.14VYY137 pKa = 10.57VFHH140 pKa = 7.26GPTASGKK147 pKa = 7.74TRR149 pKa = 11.84AAFDD153 pKa = 4.1MNCHH157 pKa = 5.16QMDD160 pKa = 4.66YY161 pKa = 10.3IAPFFTDD168 pKa = 3.59PQNAPVLLFDD178 pKa = 5.67DD179 pKa = 3.51VHH181 pKa = 7.76NPVNLFGRR189 pKa = 11.84RR190 pKa = 11.84LFLRR194 pKa = 11.84ITDD197 pKa = 4.37RR198 pKa = 11.84YY199 pKa = 7.94PMKK202 pKa = 10.46VRR204 pKa = 11.84CLGKK208 pKa = 9.8YY209 pKa = 8.62VEE211 pKa = 4.57WNPTVIIFTTNDD223 pKa = 4.3DD224 pKa = 4.12PNTWNLDD231 pKa = 3.38AACRR235 pKa = 11.84RR236 pKa = 11.84RR237 pKa = 11.84IDD239 pKa = 3.91EE240 pKa = 4.9IIAFPLDD247 pKa = 3.46KK248 pKa = 11.08GGLDD252 pKa = 3.92ALPACTQDD260 pKa = 3.16QAKK263 pKa = 10.46PGDD266 pKa = 3.87LPQCNN271 pKa = 3.48

Molecular weight: 30.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMK1|A0A0C5IMK1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 21 OX=1618245 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 9.99RR4 pKa = 11.84GRR6 pKa = 11.84SRR8 pKa = 11.84TRR10 pKa = 11.84KK11 pKa = 8.48KK12 pKa = 10.69RR13 pKa = 11.84PWQRR17 pKa = 11.84RR18 pKa = 11.84SSSGSRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 8.02PLRR30 pKa = 11.84SRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PLALKK39 pKa = 9.54QHH41 pKa = 6.65AFCEE45 pKa = 4.23RR46 pKa = 11.84AEE48 pKa = 4.04NEE50 pKa = 3.62QTIVINTEE58 pKa = 3.44AAATGLFEE66 pKa = 4.62TFTLSKK72 pKa = 9.74MRR74 pKa = 11.84QQASYY79 pKa = 9.76AQIFEE84 pKa = 4.44FYY86 pKa = 10.56RR87 pKa = 11.84IDD89 pKa = 3.52KK90 pKa = 10.36VVATFRR96 pKa = 11.84YY97 pKa = 10.08KK98 pKa = 10.61NVNNATEE105 pKa = 4.39TTGRR109 pKa = 11.84AQNEE113 pKa = 4.51VNPLLYY119 pKa = 10.58FKK121 pKa = 10.82VDD123 pKa = 3.58HH124 pKa = 7.05NDD126 pKa = 3.23NTSDD130 pKa = 3.98SLSTMKK136 pKa = 10.89DD137 pKa = 2.92SMKK140 pKa = 10.44TKK142 pKa = 8.92THH144 pKa = 6.05MFTNNNPEE152 pKa = 3.88FSIQLKK158 pKa = 9.62PAIQSEE164 pKa = 4.86MYY166 pKa = 8.89RR167 pKa = 11.84TTLTAAYY174 pKa = 7.15TPKK177 pKa = 9.05WGQWIPCDD185 pKa = 4.13DD186 pKa = 4.09PTVPHH191 pKa = 6.62YY192 pKa = 11.23GMKK195 pKa = 10.2AYY197 pKa = 10.67CCAFKK202 pKa = 10.93NADD205 pKa = 3.68WNPGSLQVSFKK216 pKa = 10.99YY217 pKa = 10.57YY218 pKa = 10.92VSFKK222 pKa = 11.05NNEE225 pKa = 3.71

Molecular weight: 26.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

496

225

271

248.0

28.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.863 ± 0.193

2.419 ± 0.403

6.048 ± 1.566

4.435 ± 0.285

5.242 ± 0.057

4.234 ± 0.705

2.016 ± 0.15

3.831 ± 0.452

5.645 ± 1.2

6.653 ± 1.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.072

6.855 ± 0.161

6.452 ± 0.702

4.234 ± 0.411

7.661 ± 0.771

5.847 ± 1.073

8.065 ± 0.518

3.831 ± 0.106

1.411 ± 0.23

4.032 ± 0.538
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski