Maize streak Reunion virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1Z771|I1Z771_9GEMI Replication-associated protein OS=Maize streak Reunion virus OX=1182518 PE=3 SV=1
MM1 pKa = 7.37SAFGNHH7 pKa = 5.61FVPTPSVQAEE17 pKa = 4.59VPTPLPGSDD26 pKa = 3.72YY27 pKa = 10.88PSEE30 pKa = 4.16EE31 pKa = 4.78DD32 pKa = 5.77DD33 pKa = 5.24LLQHH37 pKa = 6.33DD38 pKa = 4.88TPVGPDD44 pKa = 3.22PPRR47 pKa = 11.84RR48 pKa = 11.84NFQFKK53 pKa = 10.09SANAFLTYY61 pKa = 9.41PRR63 pKa = 11.84CLLTPFEE70 pKa = 4.97AGQHH74 pKa = 4.6LWEE77 pKa = 4.71VARR80 pKa = 11.84PWTPSYY86 pKa = 10.85ILASSEE92 pKa = 3.95SHH94 pKa = 6.5QDD96 pKa = 3.43GTPHH100 pKa = 6.93LHH102 pKa = 6.71VLLQTIRR109 pKa = 11.84PMSTRR114 pKa = 11.84DD115 pKa = 3.23PGFFDD120 pKa = 3.0IQGYY124 pKa = 8.96HH125 pKa = 6.89PNIQASRR132 pKa = 11.84SPNKK136 pKa = 8.48TRR138 pKa = 11.84EE139 pKa = 4.27YY140 pKa = 10.24ILKK143 pKa = 10.59SPITVYY149 pKa = 10.87SRR151 pKa = 11.84GTFIPRR157 pKa = 11.84AGTSGAGYY165 pKa = 10.62GSTPVPKK172 pKa = 10.03RR173 pKa = 11.84NEE175 pKa = 3.4IMRR178 pKa = 11.84GIIEE182 pKa = 4.49TTTSKK187 pKa = 11.24AEE189 pKa = 4.04YY190 pKa = 9.88LSEE193 pKa = 4.13VQKK196 pKa = 11.14AFPFEE201 pKa = 4.21WATKK205 pKa = 9.04LQQFEE210 pKa = 4.58YY211 pKa = 10.03SAEE214 pKa = 4.05RR215 pKa = 11.84LFPTLPSPFVPPHH228 pKa = 6.66PPSEE232 pKa = 4.59PDD234 pKa = 3.13LTCYY238 pKa = 8.01EE239 pKa = 4.94TIRR242 pKa = 11.84SWKK245 pKa = 10.08DD246 pKa = 3.03EE247 pKa = 4.47NIFQVHH253 pKa = 6.67PYY255 pKa = 10.07IYY257 pKa = 9.4MLEE260 pKa = 4.45HH261 pKa = 6.46PHH263 pKa = 5.99CQSVEE268 pKa = 3.89QAAEE272 pKa = 3.93SLKK275 pKa = 10.9WMDD278 pKa = 2.93EE279 pKa = 4.27CTRR282 pKa = 11.84GMLLHH287 pKa = 7.18APQDD291 pKa = 3.81QGASTSLGLHH301 pKa = 5.45ARR303 pKa = 11.84ARR305 pKa = 11.84LPGPGASTQQ314 pKa = 3.23

Molecular weight: 35.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1Z770|I1Z770_9GEMI Replication-associated protein OS=Maize streak Reunion virus OX=1182518 PE=3 SV=1
MM1 pKa = 7.54AGSSRR6 pKa = 11.84KK7 pKa = 7.21RR8 pKa = 11.84TRR10 pKa = 11.84RR11 pKa = 11.84GDD13 pKa = 3.44EE14 pKa = 3.96VAWNSKK20 pKa = 8.71RR21 pKa = 11.84SRR23 pKa = 11.84LSAQRR28 pKa = 11.84NPSKK32 pKa = 10.42RR33 pKa = 11.84VPRR36 pKa = 11.84NPRR39 pKa = 11.84VRR41 pKa = 11.84PSLQIQTITNGSSSMVPVKK60 pKa = 10.74QPGYY64 pKa = 10.02CGLLGTYY71 pKa = 10.13SRR73 pKa = 11.84GSDD76 pKa = 2.94EE77 pKa = 4.39NQRR80 pKa = 11.84HH81 pKa = 4.76TSEE84 pKa = 3.83TMTYY88 pKa = 10.29KK89 pKa = 10.36IALDD93 pKa = 3.36LHH95 pKa = 6.37FSITSAAAAYY105 pKa = 9.39SNSGTGVLWLIYY117 pKa = 10.09DD118 pKa = 3.92SQPNGAQPTLKK129 pKa = 10.68DD130 pKa = 2.84IFAYY134 pKa = 10.07EE135 pKa = 4.31DD136 pKa = 3.88SLVAWPYY143 pKa = 6.66TWKK146 pKa = 10.46VSRR149 pKa = 11.84EE150 pKa = 3.81VCHH153 pKa = 6.41RR154 pKa = 11.84FVVKK158 pKa = 10.56RR159 pKa = 11.84RR160 pKa = 11.84YY161 pKa = 8.55TFTLEE166 pKa = 4.1SNGRR170 pKa = 11.84RR171 pKa = 11.84NDD173 pKa = 3.37EE174 pKa = 4.06QPPSNSVWPPCKK186 pKa = 8.79THH188 pKa = 7.51LYY190 pKa = 8.75FHH192 pKa = 7.24KK193 pKa = 9.99FAKK196 pKa = 10.28GLGVRR201 pKa = 11.84TEE203 pKa = 4.13WKK205 pKa = 9.99NDD207 pKa = 3.26TAGSVGNIKK216 pKa = 10.23KK217 pKa = 9.72GALYY221 pKa = 10.24IGIAPGNGVEE231 pKa = 4.44FNVFGKK237 pKa = 8.51TRR239 pKa = 11.84LYY241 pKa = 10.33FKK243 pKa = 10.94SIGNQQ248 pKa = 3.0

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1051

102

387

262.8

29.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.994 ± 0.421

1.237 ± 0.047

4.091 ± 0.383

5.138 ± 0.799

4.662 ± 0.322

6.565 ± 0.771

2.759 ± 0.538

4.662 ± 0.525

4.186 ± 0.934

6.946 ± 0.499

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.522 ± 0.165

3.52 ± 0.895

9.99 ± 1.329

4.567 ± 0.384

6.565 ± 0.762

8.088 ± 0.759

7.326 ± 0.313

5.614 ± 0.853

1.998 ± 0.175

4.567 ± 0.427
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski