Lacinutrix sp. Bg11-31

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Lacinutrix; unclassified Lacinutrix

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8XII7|A0A2K8XII7_9FLAO Ferredoxin OS=Lacinutrix sp. Bg11-31 OX=2057808 GN=CW733_10870 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.19KK3 pKa = 10.08QLILGLAILASVFSCTTDD21 pKa = 3.26DD22 pKa = 3.83TSDD25 pKa = 2.93IVINSTTNNTTGGGGEE41 pKa = 4.37TDD43 pKa = 3.36PATIFLSGTYY53 pKa = 9.67TEE55 pKa = 5.9DD56 pKa = 3.47LTLDD60 pKa = 3.42ANNTYY65 pKa = 10.12KK66 pKa = 10.62INGSLIMASGTTLTIPPCMTIEE88 pKa = 4.24ALSSGADD95 pKa = 3.24VYY97 pKa = 11.31VAISQGAKK105 pKa = 9.78IIANGTASCPIVFTSDD121 pKa = 2.8SSNPLAGDD129 pKa = 3.14WGGLILLGKK138 pKa = 10.5APINSVTGTATATSEE153 pKa = 4.27IASLPYY159 pKa = 10.48GGNTANDD166 pKa = 3.43NSGSLSYY173 pKa = 11.21VRR175 pKa = 11.84VEE177 pKa = 4.31YY178 pKa = 10.96SGGAADD184 pKa = 4.97GQSEE188 pKa = 4.47NNGFSFYY195 pKa = 11.24GVGNGTTVNHH205 pKa = 5.88IQAIEE210 pKa = 4.09GKK212 pKa = 9.99DD213 pKa = 3.29DD214 pKa = 4.46GIEE217 pKa = 4.17FFGGTVNASFISVINAEE234 pKa = 4.64DD235 pKa = 4.63DD236 pKa = 3.96SVDD239 pKa = 3.02WTEE242 pKa = 4.47GFSGTLTDD250 pKa = 3.68VYY252 pKa = 10.61ISNRR256 pKa = 11.84ATDD259 pKa = 3.86DD260 pKa = 3.4KK261 pKa = 11.4AIEE264 pKa = 4.09ADD266 pKa = 4.02GYY268 pKa = 9.64NTDD271 pKa = 4.01FSNATGVFSKK281 pKa = 9.21PTLNNVTIVGEE292 pKa = 4.49GSANSSEE299 pKa = 4.09AVRR302 pKa = 11.84LRR304 pKa = 11.84AGTQGIFSNIHH315 pKa = 4.22ITGYY319 pKa = 11.37AEE321 pKa = 5.66GFDD324 pKa = 5.84LDD326 pKa = 5.98DD327 pKa = 5.72LDD329 pKa = 4.09TGNGVVSDD337 pKa = 4.38DD338 pKa = 3.99LQVTGVTFVDD348 pKa = 3.61VTLNMKK354 pKa = 10.4NDD356 pKa = 3.53TTVTFTIADD365 pKa = 4.89FYY367 pKa = 10.28TNEE370 pKa = 3.88GTATGTDD377 pKa = 3.68YY378 pKa = 7.61TTWGANWTVQQ388 pKa = 3.27

Molecular weight: 40.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8XDL2|A0A2K8XDL2_9FLAO Uncharacterized protein OS=Lacinutrix sp. Bg11-31 OX=2057808 GN=CW733_02270 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.92TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3170

0

3170

1077573

37

5836

339.9

38.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.433 ± 0.048

0.784 ± 0.017

5.686 ± 0.059

6.446 ± 0.047

5.242 ± 0.045

6.172 ± 0.046

1.648 ± 0.024

8.178 ± 0.041

8.068 ± 0.084

9.201 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.026

6.679 ± 0.058

3.124 ± 0.026

3.205 ± 0.025

3.026 ± 0.037

6.518 ± 0.034

6.395 ± 0.109

6.222 ± 0.042

0.949 ± 0.015

3.993 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski