Bacteroides sp. AM16-15

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; unclassified Bacteroides

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373IN54|A0A373IN54_9BACE 1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Bacteroides sp. AM16-15 OX=2292939 GN=dxr PE=3 SV=1
MM1 pKa = 6.9EE2 pKa = 5.64AKK4 pKa = 10.07VLSEE8 pKa = 3.7AKK10 pKa = 10.4VYY12 pKa = 10.67VGTYY16 pKa = 8.85GKK18 pKa = 10.85YY19 pKa = 10.57NIGSLSGAWLDD30 pKa = 4.44LSDD33 pKa = 4.65YY34 pKa = 10.83SDD36 pKa = 3.73KK37 pKa = 11.66EE38 pKa = 4.09EE39 pKa = 4.45FYY41 pKa = 9.88EE42 pKa = 4.99ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 6.63KK49 pKa = 11.13DD50 pKa = 3.63EE51 pKa = 5.18EE52 pKa = 4.44DD53 pKa = 3.48AEE55 pKa = 4.74YY56 pKa = 10.09MFQDD60 pKa = 3.69WEE62 pKa = 4.32NVPEE66 pKa = 4.11GLIGEE71 pKa = 4.39SWISEE76 pKa = 4.08NFFSLRR82 pKa = 11.84DD83 pKa = 3.72AVEE86 pKa = 4.53DD87 pKa = 4.41LSDD90 pKa = 3.72TEE92 pKa = 4.18QEE94 pKa = 5.68AFFVWCNYY102 pKa = 9.3KK103 pKa = 10.56SRR105 pKa = 11.84DD106 pKa = 3.77LSEE109 pKa = 4.74EE110 pKa = 3.95DD111 pKa = 5.29ADD113 pKa = 5.83DD114 pKa = 4.15LVCDD118 pKa = 5.7FRR120 pKa = 11.84DD121 pKa = 4.05EE122 pKa = 4.35YY123 pKa = 10.73QGEE126 pKa = 4.18YY127 pKa = 10.64DD128 pKa = 4.42DD129 pKa = 5.91EE130 pKa = 4.89EE131 pKa = 5.7DD132 pKa = 3.23FAYY135 pKa = 10.08EE136 pKa = 4.43IIEE139 pKa = 4.11EE140 pKa = 4.66CYY142 pKa = 10.66DD143 pKa = 3.52LPEE146 pKa = 4.02FAKK149 pKa = 10.18TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 5.04LFMCDD165 pKa = 2.54YY166 pKa = 9.94WFDD169 pKa = 3.78DD170 pKa = 3.9GFVFRR175 pKa = 11.84AAA177 pKa = 3.87

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373IVH1|A0A373IVH1_9BACE Uncharacterized protein OS=Bacteroides sp. AM16-15 OX=2292939 GN=DW175_10335 PE=4 SV=1
MM1 pKa = 7.2NRR3 pKa = 11.84NEE5 pKa = 4.35RR6 pKa = 11.84DD7 pKa = 3.2FSNTFRR13 pKa = 11.84NRR15 pKa = 11.84FFASTDD21 pKa = 3.1NRR23 pKa = 11.84GVTFAAFLSALRR35 pKa = 11.84TKK37 pKa = 10.6SSAVAPSKK45 pKa = 9.95TGMFLIVIAMILFFAKK61 pKa = 10.03IQFCCYY67 pKa = 6.98TAKK70 pKa = 10.82

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3417

0

3417

1213394

18

2060

355.1

40.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.018 ± 0.043

1.273 ± 0.014

5.398 ± 0.027

6.766 ± 0.043

4.542 ± 0.026

6.692 ± 0.047

1.951 ± 0.017

7.052 ± 0.038

6.95 ± 0.035

9.132 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.02

5.136 ± 0.037

3.687 ± 0.023

3.438 ± 0.024

4.585 ± 0.03

5.949 ± 0.033

5.564 ± 0.032

6.362 ± 0.04

1.223 ± 0.018

4.455 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski