Amino acid dipepetide frequency for Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.999AlaAla: 8.999 ± 0.319	1.274AlaCys: 1.274 ± 0.04	4.607AlaAsp: 4.607 ± 0.07	5.359AlaGlu: 5.359 ± 0.071	3.226AlaPhe: 3.226 ± 0.063	8.074AlaGly: 8.074 ± 0.114	1.256AlaHis: 1.256 ± 0.033	6.508AlaIle: 6.508 ± 0.1	3.963AlaLys: 3.963 ± 0.071	8.201AlaLeu: 8.201 ± 0.125	2.805AlaMet: 2.805 ± 0.055	2.27AlaAsn: 2.27 ± 0.056	3.261AlaPro: 3.261 ± 0.077	2.119AlaGln: 2.119 ± 0.064	5.234AlaArg: 5.234 ± 0.086	5.247AlaSer: 5.247 ± 0.096	4.523AlaThr: 4.523 ± 0.084	6.638AlaVal: 6.638 ± 0.108	0.834AlaTrp: 0.834 ± 0.035	2.75AlaTyr: 2.75 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.973CysAla: 0.973 ± 0.035	0.249CysCys: 0.249 ± 0.017	0.757CysAsp: 0.757 ± 0.033	0.703CysGlu: 0.703 ± 0.028	0.416CysPhe: 0.416 ± 0.024	1.45CysGly: 1.45 ± 0.046	0.296CysHis: 0.296 ± 0.023	0.838CysIle: 0.838 ± 0.031	0.644CysLys: 0.644 ± 0.034	1.035CysLeu: 1.035 ± 0.037	0.356CysMet: 0.356 ± 0.021	0.447CysAsn: 0.447 ± 0.023	0.902CysPro: 0.902 ± 0.037	0.317CysGln: 0.317 ± 0.019	0.735CysArg: 0.735 ± 0.028	0.75CysSer: 0.75 ± 0.03	0.737CysThr: 0.737 ± 0.031	0.833CysVal: 0.833 ± 0.032	0.103CysTrp: 0.103 ± 0.01	0.429CysTyr: 0.429 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	4.523AspAla: 4.523 ± 0.074	0.659AspCys: 0.659 ± 0.026	2.837AspAsp: 2.837 ± 0.062	3.623AspGlu: 3.623 ± 0.059	2.077AspPhe: 2.077 ± 0.044	4.303AspGly: 4.303 ± 0.078	1.074AspHis: 1.074 ± 0.035	4.229AspIle: 4.229 ± 0.076	2.627AspLys: 2.627 ± 0.057	5.367AspLeu: 5.367 ± 0.084	1.682AspMet: 1.682 ± 0.04	1.885AspAsn: 1.885 ± 0.054	2.579AspPro: 2.579 ± 0.055	1.447AspGln: 1.447 ± 0.042	3.725AspArg: 3.725 ± 0.074	3.051AspSer: 3.051 ± 0.055	2.844AspThr: 2.844 ± 0.058	4.471AspVal: 4.471 ± 0.079	0.563AspTrp: 0.563 ± 0.026	2.131AspTyr: 2.131 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	5.885GluAla: 5.885 ± 0.081	0.629GluCys: 0.629 ± 0.028	3.075GluAsp: 3.075 ± 0.057	4.387GluGlu: 4.387 ± 0.093	2.082GluPhe: 2.082 ± 0.048	3.924GluGly: 3.924 ± 0.075	1.255GluHis: 1.255 ± 0.04	4.77GluIle: 4.77 ± 0.082	4.755GluLys: 4.755 ± 0.095	5.661GluLeu: 5.661 ± 0.097	1.946GluMet: 1.946 ± 0.043	2.291GluAsn: 2.291 ± 0.042	2.346GluPro: 2.346 ± 0.056	1.971GluGln: 1.971 ± 0.057	3.352GluArg: 3.352 ± 0.071	3.268GluSer: 3.268 ± 0.069	3.081GluThr: 3.081 ± 0.066	4.378GluVal: 4.378 ± 0.075	0.551GluTrp: 0.551 ± 0.025	2.23GluTyr: 2.23 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.804PheAla: 2.804 ± 0.056	0.492PheCys: 0.492 ± 0.025	2.27PheAsp: 2.27 ± 0.046	2.049PheGlu: 2.049 ± 0.048	1.556PhePhe: 1.556 ± 0.049	3.157PheGly: 3.157 ± 0.063	0.664PheHis: 0.664 ± 0.028	2.645PheIle: 2.645 ± 0.058	1.868PheLys: 1.868 ± 0.048	3.431PheLeu: 3.431 ± 0.07	0.979PheMet: 0.979 ± 0.032	1.391PheAsn: 1.391 ± 0.035	1.417PhePro: 1.417 ± 0.044	0.848PheGln: 0.848 ± 0.031	1.802PheArg: 1.802 ± 0.043	2.63PheSer: 2.63 ± 0.058	2.269PheThr: 2.269 ± 0.06	2.685PheVal: 2.685 ± 0.06	0.37PheTrp: 0.37 ± 0.02	1.342PheTyr: 1.342 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	5.813GlyAla: 5.813 ± 0.096	1.246GlyCys: 1.246 ± 0.042	4.081GlyAsp: 4.081 ± 0.079	4.58GlyGlu: 4.58 ± 0.073	3.135GlyPhe: 3.135 ± 0.073	5.555GlyGly: 5.555 ± 0.127	1.477GlyHis: 1.477 ± 0.041	6.196GlyIle: 6.196 ± 0.081	5.075GlyLys: 5.075 ± 0.086	7.145GlyLeu: 7.145 ± 0.113	2.546GlyMet: 2.546 ± 0.058	2.855GlyAsn: 2.855 ± 0.065	2.534GlyPro: 2.534 ± 0.06	2.119GlyGln: 2.119 ± 0.051	4.302GlyArg: 4.302 ± 0.067	4.988GlySer: 4.988 ± 0.083	4.782GlyThr: 4.782 ± 0.075	5.575GlyVal: 5.575 ± 0.07	0.902GlyTrp: 0.902 ± 0.04	3.175GlyTyr: 3.175 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.392HisAla: 1.392 ± 0.037	0.291HisCys: 0.291 ± 0.019	1.021HisAsp: 1.021 ± 0.034	1.063HisGlu: 1.063 ± 0.029	0.675HisPhe: 0.675 ± 0.03	1.495HisGly: 1.495 ± 0.049	0.418HisHis: 0.418 ± 0.025	1.18HisIle: 1.18 ± 0.037	0.861HisLys: 0.861 ± 0.032	1.464HisLeu: 1.464 ± 0.04	0.534HisMet: 0.534 ± 0.024	0.58HisAsn: 0.58 ± 0.024	1.069HisPro: 1.069 ± 0.04	0.438HisGln: 0.438 ± 0.022	1.034HisArg: 1.034 ± 0.038	0.971HisSer: 0.971 ± 0.032	0.948HisThr: 0.948 ± 0.029	1.315HisVal: 1.315 ± 0.039	0.155HisTrp: 0.155 ± 0.014	0.683HisTyr: 0.683 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.682IleAla: 6.682 ± 0.092	0.928IleCys: 0.928 ± 0.034	4.338IleAsp: 4.338 ± 0.074	4.467IleGlu: 4.467 ± 0.085	2.586IlePhe: 2.586 ± 0.063	5.532IleGly: 5.532 ± 0.085	1.099IleHis: 1.099 ± 0.036	5.076IleIle: 5.076 ± 0.089	3.676IleLys: 3.676 ± 0.072	6.112IleLeu: 6.112 ± 0.092	1.963IleMet: 1.963 ± 0.048	2.551IleAsn: 2.551 ± 0.055	3.199IlePro: 3.199 ± 0.05	1.62IleGln: 1.62 ± 0.046	3.981IleArg: 3.981 ± 0.066	4.733IleSer: 4.733 ± 0.084	4.331IleThr: 4.331 ± 0.066	6.088IleVal: 6.088 ± 0.089	0.565IleTrp: 0.565 ± 0.026	2.3IleTyr: 2.3 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	5.21LysAla: 5.21 ± 0.073	0.656LysCys: 0.656 ± 0.031	3.329LysAsp: 3.329 ± 0.06	3.95LysGlu: 3.95 ± 0.089	1.642LysPhe: 1.642 ± 0.039	3.911LysGly: 3.911 ± 0.067	0.962LysHis: 0.962 ± 0.03	3.788LysIle: 3.788 ± 0.06	4.141LysLys: 4.141 ± 0.086	4.567LysLeu: 4.567 ± 0.079	1.715LysMet: 1.715 ± 0.045	2.319LysAsn: 2.319 ± 0.055	2.591LysPro: 2.591 ± 0.058	1.478LysGln: 1.478 ± 0.04	2.546LysArg: 2.546 ± 0.063	3.122LysSer: 3.122 ± 0.061	3.054LysThr: 3.054 ± 0.06	4.086LysVal: 4.086 ± 0.066	0.458LysTrp: 0.458 ± 0.024	2.124LysTyr: 2.124 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	8.462LeuAla: 8.462 ± 0.122	1.114LeuCys: 1.114 ± 0.042	4.957LeuAsp: 4.957 ± 0.08	5.323LeuGlu: 5.323 ± 0.089	3.54LeuPhe: 3.54 ± 0.073	6.431LeuGly: 6.431 ± 0.101	1.475LeuHis: 1.475 ± 0.045	6.625LeuIle: 6.625 ± 0.11	5.854LeuLys: 5.854 ± 0.095	9.509LeuLeu: 9.509 ± 0.146	2.561LeuMet: 2.561 ± 0.057	3.315LeuAsn: 3.315 ± 0.061	4.206LeuPro: 4.206 ± 0.076	2.55LeuGln: 2.55 ± 0.055	4.77LeuArg: 4.77 ± 0.079	6.42LeuSer: 6.42 ± 0.1	4.922LeuThr: 4.922 ± 0.079	6.382LeuVal: 6.382 ± 0.086	0.838LeuTrp: 0.838 ± 0.031	3.146LeuTyr: 3.146 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.943MetAla: 2.943 ± 0.06	0.335MetCys: 0.335 ± 0.019	1.679MetAsp: 1.679 ± 0.042	1.693MetGlu: 1.693 ± 0.047	0.926MetPhe: 0.926 ± 0.034	2.224MetGly: 2.224 ± 0.048	0.497MetHis: 0.497 ± 0.023	2.234MetIle: 2.234 ± 0.05	1.788MetLys: 1.788 ± 0.045	2.788MetLeu: 2.788 ± 0.058	0.863MetMet: 0.863 ± 0.034	1.026MetAsn: 1.026 ± 0.03	1.502MetPro: 1.502 ± 0.049	0.828MetGln: 0.828 ± 0.03	1.487MetArg: 1.487 ± 0.037	1.91MetSer: 1.91 ± 0.043	1.77MetThr: 1.77 ± 0.049	2.091MetVal: 2.091 ± 0.053	0.208MetTrp: 0.208 ± 0.016	0.881MetTyr: 0.881 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.758AsnAla: 2.758 ± 0.058	0.412AsnCys: 0.412 ± 0.022	1.692AsnAsp: 1.692 ± 0.046	1.774AsnGlu: 1.774 ± 0.045	1.108AsnPhe: 1.108 ± 0.038	2.8AsnGly: 2.8 ± 0.056	0.552AsnHis: 0.552 ± 0.028	2.577AsnIle: 2.577 ± 0.058	1.613AsnLys: 1.613 ± 0.041	3.167AsnLeu: 3.167 ± 0.067	1.034AsnMet: 1.034 ± 0.029	1.48AsnAsn: 1.48 ± 0.057	1.958AsnPro: 1.958 ± 0.048	0.901AsnGln: 0.901 ± 0.033	1.851AsnArg: 1.851 ± 0.044	2.061AsnSer: 2.061 ± 0.062	1.943AsnThr: 1.943 ± 0.053	3.009AsnVal: 3.009 ± 0.065	0.361AsnTrp: 0.361 ± 0.021	1.245AsnTyr: 1.245 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.561ProAla: 4.561 ± 0.101	0.518ProCys: 0.518 ± 0.024	3.073ProAsp: 3.073 ± 0.065	3.854ProGlu: 3.854 ± 0.072	1.702ProPhe: 1.702 ± 0.045	4.586ProGly: 4.586 ± 0.091	0.775ProHis: 0.775 ± 0.03	2.368ProIle: 2.368 ± 0.056	1.872ProLys: 1.872 ± 0.05	3.72ProLeu: 3.72 ± 0.073	1.078ProMet: 1.078 ± 0.033	1.057ProAsn: 1.057 ± 0.039	1.892ProPro: 1.892 ± 0.055	1.232ProGln: 1.232 ± 0.044	1.938ProArg: 1.938 ± 0.047	2.373ProSer: 2.373 ± 0.064	2.613ProThr: 2.613 ± 0.125	4.368ProVal: 4.368 ± 0.069	0.414ProTrp: 0.414 ± 0.023	1.626ProTyr: 1.626 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.614GlnAla: 2.614 ± 0.069	0.335GlnCys: 0.335 ± 0.019	1.298GlnAsp: 1.298 ± 0.037	1.592GlnGlu: 1.592 ± 0.044	1.043GlnPhe: 1.043 ± 0.04	1.78GlnGly: 1.78 ± 0.044	0.501GlnHis: 0.501 ± 0.024	1.963GlnIle: 1.963 ± 0.05	1.752GlnLys: 1.752 ± 0.046	2.183GlnLeu: 2.183 ± 0.046	0.921GlnMet: 0.921 ± 0.041	0.977GlnAsn: 0.977 ± 0.036	1.275GlnPro: 1.275 ± 0.048	1.072GlnGln: 1.072 ± 0.057	1.41GlnArg: 1.41 ± 0.043	1.604GlnSer: 1.604 ± 0.05	1.387GlnThr: 1.387 ± 0.044	1.978GlnVal: 1.978 ± 0.046	0.269GlnTrp: 0.269 ± 0.015	1.135GlnTyr: 1.135 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	4.17ArgAla: 4.17 ± 0.071	0.744ArgCys: 0.744 ± 0.034	3.081ArgAsp: 3.081 ± 0.061	4.097ArgGlu: 4.097 ± 0.084	1.941ArgPhe: 1.941 ± 0.047	3.399ArgGly: 3.399 ± 0.063	1.07ArgHis: 1.07 ± 0.034	3.933ArgIle: 3.933 ± 0.073	3.57ArgLys: 3.57 ± 0.074	5.26ArgLeu: 5.26 ± 0.089	1.861ArgMet: 1.861 ± 0.048	1.877ArgAsn: 1.877 ± 0.05	2.24ArgPro: 2.24 ± 0.055	1.924ArgGln: 1.924 ± 0.054	3.282ArgArg: 3.282 ± 0.074	3.236ArgSer: 3.236 ± 0.06	2.641ArgThr: 2.641 ± 0.053	3.527ArgVal: 3.527 ± 0.059	0.518ArgTrp: 0.518 ± 0.023	2.09ArgTyr: 2.09 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	5.014SerAla: 5.014 ± 0.08	0.689SerCys: 0.689 ± 0.029	3.344SerAsp: 3.344 ± 0.073	3.258SerGlu: 3.258 ± 0.061	2.314SerPhe: 2.314 ± 0.055	5.642SerGly: 5.642 ± 0.083	1.146SerHis: 1.146 ± 0.04	4.439SerIle: 4.439 ± 0.077	2.87SerLys: 2.87 ± 0.057	6.015SerLeu: 6.015 ± 0.092	1.974SerMet: 1.974 ± 0.048	1.881SerAsn: 1.881 ± 0.046	3.131SerPro: 3.131 ± 0.066	1.744SerGln: 1.744 ± 0.043	3.447SerArg: 3.447 ± 0.068	3.865SerSer: 3.865 ± 0.082	3.523SerThr: 3.523 ± 0.07	4.51SerVal: 4.51 ± 0.073	0.61SerTrp: 0.61 ± 0.028	2.15SerTyr: 2.15 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	4.853ThrAla: 4.853 ± 0.081	0.725ThrCys: 0.725 ± 0.026	3.059ThrAsp: 3.059 ± 0.058	2.826ThrGlu: 2.826 ± 0.051	2.158ThrPhe: 2.158 ± 0.056	5.497ThrGly: 5.497 ± 0.089	0.902ThrHis: 0.902 ± 0.032	4.292ThrIle: 4.292 ± 0.077	2.154ThrLys: 2.154 ± 0.049	5.198ThrLeu: 5.198 ± 0.079	1.487ThrMet: 1.487 ± 0.048	1.631ThrAsn: 1.631 ± 0.041	3.703ThrPro: 3.703 ± 0.135	1.229ThrGln: 1.229 ± 0.048	2.659ThrArg: 2.659 ± 0.058	3.379ThrSer: 3.379 ± 0.061	3.298ThrThr: 3.298 ± 0.07	4.784ThrVal: 4.784 ± 0.1	0.49ThrTrp: 0.49 ± 0.026	1.91ThrTyr: 1.91 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	6.196ValAla: 6.196 ± 0.095	1.059ValCys: 1.059 ± 0.036	4.334ValAsp: 4.334 ± 0.071	4.728ValGlu: 4.728 ± 0.092	2.843ValPhe: 2.843 ± 0.05	4.688ValGly: 4.688 ± 0.081	1.27ValHis: 1.27 ± 0.037	5.545ValIle: 5.545 ± 0.095	4.375ValLys: 4.375 ± 0.075	7.144ValLeu: 7.144 ± 0.097	2.088ValMet: 2.088 ± 0.05	2.641ValAsn: 2.641 ± 0.059	3.806ValPro: 3.806 ± 0.069	1.906ValGln: 1.906 ± 0.045	4.244ValArg: 4.244 ± 0.083	5.097ValSer: 5.097 ± 0.077	4.953ValThr: 4.953 ± 0.092	5.682ValVal: 5.682 ± 0.093	0.701ValTrp: 0.701 ± 0.031	2.559ValTyr: 2.559 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.674TrpAla: 0.674 ± 0.026	0.121TrpCys: 0.121 ± 0.014	0.566TrpAsp: 0.566 ± 0.027	0.532TrpGlu: 0.532 ± 0.026	0.325TrpPhe: 0.325 ± 0.018	0.714TrpGly: 0.714 ± 0.035	0.18TrpHis: 0.18 ± 0.014	0.647TrpIle: 0.647 ± 0.026	0.608TrpLys: 0.608 ± 0.028	0.929TrpLeu: 0.929 ± 0.032	0.325TrpMet: 0.325 ± 0.017	0.393TrpAsn: 0.393 ± 0.022	0.352TrpPro: 0.352 ± 0.021	0.346TrpGln: 0.346 ± 0.021	0.471TrpArg: 0.471 ± 0.024	0.561TrpSer: 0.561 ± 0.028	0.502TrpThr: 0.502 ± 0.025	0.653TrpVal: 0.653 ± 0.03	0.139TrpTrp: 0.139 ± 0.014	0.388TrpTyr: 0.388 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.623TyrAla: 2.623 ± 0.053	0.494TyrCys: 0.494 ± 0.025	2.297TyrAsp: 2.297 ± 0.06	1.979TyrGlu: 1.979 ± 0.055	1.373TyrPhe: 1.373 ± 0.042	2.921TyrGly: 2.921 ± 0.054	0.718TyrHis: 0.718 ± 0.029	1.977TyrIle: 1.977 ± 0.051	1.584TyrLys: 1.584 ± 0.046	3.503TyrLeu: 3.503 ± 0.07	0.954TyrMet: 0.954 ± 0.033	1.428TyrAsn: 1.428 ± 0.042	1.658TyrPro: 1.658 ± 0.053	0.999TyrGln: 0.999 ± 0.035	2.19TyrArg: 2.19 ± 0.057	2.3TyrSer: 2.3 ± 0.062	2.113TyrThr: 2.113 ± 0.05	2.806TyrVal: 2.806 ± 0.058	0.376TyrTrp: 0.376 ± 0.022	1.604TyrTyr: 1.604 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3071 proteins (889879 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---