Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-J19))

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus H

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|Q45UF4|NSP2_ROTJ1 Non-structural protein 2 OS=Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) OX=335103 PE=3 SV=1
MM1 pKa = 7.7LLFIILSVGVDD12 pKa = 4.25AISYY16 pKa = 8.33IQNDD20 pKa = 3.93RR21 pKa = 11.84SNDD24 pKa = 3.24LCIVYY29 pKa = 10.31EE30 pKa = 4.1MTSFGTSFNNANDD43 pKa = 4.0SLVKK47 pKa = 10.02LHH49 pKa = 6.26KK50 pKa = 10.43HH51 pKa = 5.35MGLKK55 pKa = 10.53YY56 pKa = 8.56EE57 pKa = 4.06VCKK60 pKa = 10.63IEE62 pKa = 4.59SNANALTQMQKK73 pKa = 10.65CNCIYY78 pKa = 10.84DD79 pKa = 3.9DD80 pKa = 3.83TPQIVVFTNFKK91 pKa = 10.35KK92 pKa = 10.84SSLKK96 pKa = 9.86TLIGTEE102 pKa = 4.27NKK104 pKa = 10.25CEE106 pKa = 4.4LLPQTTIYY114 pKa = 9.93TPTVDD119 pKa = 3.67IEE121 pKa = 4.43SEE123 pKa = 4.23YY124 pKa = 10.65FIYY127 pKa = 10.84GNDD130 pKa = 3.27VKK132 pKa = 10.65ICYY135 pKa = 9.55LDD137 pKa = 3.8KK138 pKa = 10.99NLLGIGCDD146 pKa = 3.65ATDD149 pKa = 3.49TTSWLDD155 pKa = 3.57LDD157 pKa = 5.21AGLPTNHH164 pKa = 7.18ALDD167 pKa = 3.8IPEE170 pKa = 4.04ITSDD174 pKa = 3.72GFKK177 pKa = 10.96LFAKK181 pKa = 10.44YY182 pKa = 10.22SDD184 pKa = 3.92SFLCQRR190 pKa = 11.84LMDD193 pKa = 4.14EE194 pKa = 4.83PKK196 pKa = 10.66KK197 pKa = 10.58QIQFYY202 pKa = 11.08AEE204 pKa = 3.92VDD206 pKa = 3.68NVPSNDD212 pKa = 3.39VIEE215 pKa = 4.87SSRR218 pKa = 11.84SWASVWKK225 pKa = 9.76VVKK228 pKa = 9.48TVLHH232 pKa = 5.47FTYY235 pKa = 10.35HH236 pKa = 6.84ILDD239 pKa = 4.0LFYY242 pKa = 11.15GNRR245 pKa = 11.84RR246 pKa = 11.84ATARR250 pKa = 11.84MIEE253 pKa = 4.08HH254 pKa = 6.6SPLGG258 pKa = 3.71

Molecular weight:
29.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|Q5Y9A9|NSP3_ROTJ1 Non-structural protein 3 OS=Rotavirus X (strain RVX/Human/China/NADRV-J19/1997/GXP[X]) OX=335103 PE=3 SV=1
MM1 pKa = 7.46EE2 pKa = 5.77PEE4 pKa = 5.3NYY6 pKa = 9.97LAWLARR12 pKa = 11.84DD13 pKa = 3.39IVRR16 pKa = 11.84NLSYY20 pKa = 10.97TSLVYY25 pKa = 10.81NNPKK29 pKa = 9.55VAIVEE34 pKa = 4.04LLDD37 pKa = 3.62NKK39 pKa = 10.39EE40 pKa = 3.94AFFAYY45 pKa = 9.65EE46 pKa = 4.35KK47 pKa = 9.97EE48 pKa = 4.32QKK50 pKa = 9.36TPEE53 pKa = 3.84ALINYY58 pKa = 8.46IDD60 pKa = 4.83SIVKK64 pKa = 10.34SSISVEE70 pKa = 4.21DD71 pKa = 4.19KK72 pKa = 11.0IEE74 pKa = 4.01ALLKK78 pKa = 9.63IRR80 pKa = 11.84YY81 pKa = 8.73ISVYY85 pKa = 10.67VDD87 pKa = 3.48DD88 pKa = 5.96KK89 pKa = 11.35SDD91 pKa = 3.21KK92 pKa = 10.6RR93 pKa = 11.84DD94 pKa = 3.24IVLQLLNRR102 pKa = 11.84TIKK105 pKa = 10.58KK106 pKa = 9.8IEE108 pKa = 4.34LKK110 pKa = 10.11TKK112 pKa = 10.49ISDD115 pKa = 3.92EE116 pKa = 4.19LNDD119 pKa = 5.1AINAITIEE127 pKa = 4.2SKK129 pKa = 9.69NWKK132 pKa = 8.2IQNSKK137 pKa = 9.86SFKK140 pKa = 9.22PYY142 pKa = 10.11HH143 pKa = 5.76YY144 pKa = 9.69NQLVSDD150 pKa = 4.36FIKK153 pKa = 10.7YY154 pKa = 10.5NEE156 pKa = 4.03FEE158 pKa = 4.39VLEE161 pKa = 4.51GTDD164 pKa = 3.57PLKK167 pKa = 10.62WKK169 pKa = 10.67SDD171 pKa = 3.61TLQGLSPNYY180 pKa = 9.03NHH182 pKa = 6.95RR183 pKa = 11.84THH185 pKa = 6.5TLISSIIYY193 pKa = 7.14ATSVRR198 pKa = 11.84FDD200 pKa = 3.58NYY202 pKa = 10.75NDD204 pKa = 3.49EE205 pKa = 4.15QLQVLLYY212 pKa = 9.04LFSVIRR218 pKa = 11.84TNYY221 pKa = 8.24VNGYY225 pKa = 10.15LEE227 pKa = 4.16ILPNRR232 pKa = 11.84KK233 pKa = 8.51WSHH236 pKa = 5.57SLADD240 pKa = 3.54LRR242 pKa = 11.84EE243 pKa = 4.27NKK245 pKa = 10.34SIMMYY250 pKa = 8.9SAKK253 pKa = 9.91IIHH256 pKa = 7.0ASCAMISILHH266 pKa = 6.46AVPIDD271 pKa = 3.7YY272 pKa = 10.59FFLAQIIASFSEE284 pKa = 4.52IPAHH288 pKa = 5.87AAKK291 pKa = 10.27HH292 pKa = 5.53LSSPMTLYY300 pKa = 10.33IGIAQLRR307 pKa = 11.84SNIVVSTKK315 pKa = 9.92IAAEE319 pKa = 4.22SVATEE324 pKa = 4.1SPNISRR330 pKa = 11.84LEE332 pKa = 3.79EE333 pKa = 3.88SQIRR337 pKa = 11.84EE338 pKa = 4.16WEE340 pKa = 4.18QEE342 pKa = 3.59MSEE345 pKa = 4.5YY346 pKa = 10.3PFQSSRR352 pKa = 11.84MVRR355 pKa = 11.84MMKK358 pKa = 10.45KK359 pKa = 10.16NIFDD363 pKa = 3.62VSVDD367 pKa = 3.14MFYY370 pKa = 10.93AIFNCFSATFHH381 pKa = 5.9VGHH384 pKa = 7.3RR385 pKa = 11.84IDD387 pKa = 4.2NPQDD391 pKa = 3.85AIEE394 pKa = 4.35AQVKK398 pKa = 9.37VEE400 pKa = 3.99YY401 pKa = 10.21TSDD404 pKa = 3.39VDD406 pKa = 4.52KK407 pKa = 11.43EE408 pKa = 4.44MYY410 pKa = 9.39DD411 pKa = 3.01QYY413 pKa = 11.88YY414 pKa = 10.5FLLKK418 pKa = 10.67RR419 pKa = 11.84MLTDD423 pKa = 3.02QLAEE427 pKa = 4.24YY428 pKa = 10.3AEE430 pKa = 4.03EE431 pKa = 4.9MYY433 pKa = 10.57FKK435 pKa = 11.09YY436 pKa = 10.85NSDD439 pKa = 3.22VTAEE443 pKa = 4.22SLAAMANSSNGYY455 pKa = 8.61SRR457 pKa = 11.84SVTFLDD463 pKa = 5.08RR464 pKa = 11.84EE465 pKa = 4.11IKK467 pKa = 7.0TTKK470 pKa = 10.6KK471 pKa = 9.58MLHH474 pKa = 6.67LDD476 pKa = 3.84DD477 pKa = 6.22DD478 pKa = 4.59LSKK481 pKa = 11.26NLNFTNIGDD490 pKa = 4.04QIKK493 pKa = 10.44KK494 pKa = 9.16GIPMGTRR501 pKa = 11.84NVPARR506 pKa = 11.84QTRR509 pKa = 11.84GIFILSWQVAAIQHH523 pKa = 6.29TIAEE527 pKa = 4.27FLYY530 pKa = 10.6KK531 pKa = 9.96KK532 pKa = 10.11AKK534 pKa = 9.99KK535 pKa = 10.15GGFGATFAEE544 pKa = 4.84AYY546 pKa = 8.25VAKK549 pKa = 10.32AATLTYY555 pKa = 10.43GILAEE560 pKa = 4.34ATSKK564 pKa = 11.11ADD566 pKa = 3.65QLILYY571 pKa = 8.59TDD573 pKa = 3.83VSQWDD578 pKa = 3.7ASQHH582 pKa = 4.09NTEE585 pKa = 4.89PYY587 pKa = 9.38RR588 pKa = 11.84SAWINAIKK596 pKa = 10.08EE597 pKa = 3.85ARR599 pKa = 11.84TKK601 pKa = 11.14YY602 pKa = 9.99KK603 pKa = 10.28INYY606 pKa = 7.3NQEE609 pKa = 3.97PVVLGMNVLDD619 pKa = 5.15KK620 pKa = 10.67MIEE623 pKa = 3.92IQEE626 pKa = 4.22ALLNSNLIVEE636 pKa = 4.62SQGSKK641 pKa = 9.77RR642 pKa = 11.84QPLRR646 pKa = 11.84IKK648 pKa = 9.73YY649 pKa = 9.41HH650 pKa = 5.48GVASGEE656 pKa = 4.15KK657 pKa = 6.55TTKK660 pKa = 10.0IGNSFANVALITTVFNNLTNTMPSIRR686 pKa = 11.84VNHH689 pKa = 5.67MRR691 pKa = 11.84VDD693 pKa = 3.56GDD695 pKa = 4.26DD696 pKa = 3.95NVVTMYY702 pKa = 8.46TANRR706 pKa = 11.84IDD708 pKa = 3.69EE709 pKa = 4.2VQEE712 pKa = 4.15NIKK715 pKa = 10.47EE716 pKa = 4.08KK717 pKa = 9.82YY718 pKa = 9.85KK719 pKa = 11.03RR720 pKa = 11.84MNAKK724 pKa = 9.63VKK726 pKa = 10.64ALASYY731 pKa = 9.47TGLEE735 pKa = 3.81MAKK738 pKa = 10.23RR739 pKa = 11.84FIICGKK745 pKa = 8.84IFEE748 pKa = 5.12RR749 pKa = 11.84GAISIFTAEE758 pKa = 4.28RR759 pKa = 11.84PYY761 pKa = 10.7GTDD764 pKa = 3.38LSVQSTTGSLIYY776 pKa = 10.34SAAVNAYY783 pKa = 10.03RR784 pKa = 11.84GFGDD788 pKa = 3.23NYY790 pKa = 10.3LNFMTDD796 pKa = 2.95VLVPPSASVKK806 pKa = 8.23ITGRR810 pKa = 11.84LRR812 pKa = 11.84SLLSPVTLYY821 pKa = 9.93STGPLSFEE829 pKa = 3.53ITPYY833 pKa = 10.94GLGGRR838 pKa = 11.84MRR840 pKa = 11.84LFSLSKK846 pKa = 11.03EE847 pKa = 3.83NMEE850 pKa = 4.64LYY852 pKa = 10.53KK853 pKa = 10.48ILTSSLAISVQPDD866 pKa = 3.56EE867 pKa = 4.24IKK869 pKa = 10.5KK870 pKa = 10.65YY871 pKa = 10.93SSTPQFKK878 pKa = 10.71ARR880 pKa = 11.84VDD882 pKa = 3.6RR883 pKa = 11.84MISSVQIAMKK893 pKa = 10.45SEE895 pKa = 3.81AKK897 pKa = 10.19IITSILRR904 pKa = 11.84DD905 pKa = 3.5KK906 pKa = 11.2EE907 pKa = 4.15EE908 pKa = 3.97QKK910 pKa = 9.79TLGVPNVATTKK921 pKa = 10.23NRR923 pKa = 11.84QQIEE927 pKa = 4.36KK928 pKa = 10.43ARR930 pKa = 11.84KK931 pKa = 6.86TLSLPKK937 pKa = 9.23EE938 pKa = 4.22TLPKK942 pKa = 7.68VTKK945 pKa = 10.04YY946 pKa = 9.4YY947 pKa = 10.06PEE949 pKa = 4.86EE950 pKa = 4.0IFHH953 pKa = 7.04LILRR957 pKa = 11.84NSTFTIPKK965 pKa = 9.0LNTMTKK971 pKa = 10.29VYY973 pKa = 9.62MNNSANITKK982 pKa = 9.83LQQQLGVRR990 pKa = 11.84VSSGIQVHH998 pKa = 6.02RR999 pKa = 11.84PVNTLLKK1006 pKa = 10.27LVEE1009 pKa = 4.09KK1010 pKa = 10.25HH1011 pKa = 6.21SPIKK1015 pKa = 10.33ISPSDD1020 pKa = 3.49LVLYY1024 pKa = 9.77SKK1026 pKa = 10.84KK1027 pKa = 10.5YY1028 pKa = 10.93DD1029 pKa = 3.52LTNLNGKK1036 pKa = 8.57KK1037 pKa = 9.66QFLIDD1042 pKa = 4.27LGISGNEE1049 pKa = 3.27LRR1051 pKa = 11.84FYY1053 pKa = 11.2LNSKK1057 pKa = 10.15LLFHH1061 pKa = 7.52DD1062 pKa = 5.02LLLSKK1067 pKa = 10.53YY1068 pKa = 9.78DD1069 pKa = 3.89KK1070 pKa = 10.82LYY1072 pKa = 10.32EE1073 pKa = 4.32SPGFGATQLNALPLDD1088 pKa = 3.78LTAAEE1093 pKa = 4.59KK1094 pKa = 10.98VFSIKK1099 pKa = 10.75LNLPNTYY1106 pKa = 10.33YY1107 pKa = 10.77EE1108 pKa = 4.47LLMLILLYY1116 pKa = 10.51EE1117 pKa = 4.11YY1118 pKa = 11.42VNFVMFTGDD1127 pKa = 3.21TFRR1130 pKa = 11.84AVCIPEE1136 pKa = 4.14SQTINAKK1143 pKa = 9.63LVKK1146 pKa = 9.55TVMTMIDD1153 pKa = 4.35NIQLDD1158 pKa = 4.0TVMFSDD1164 pKa = 5.27NIYY1167 pKa = 11.09

Molecular weight:
132.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5679

176

1167

516.3

58.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.758 ± 0.667

1.233 ± 0.379

6.093 ± 0.506

5.864 ± 0.41

4.543 ± 0.363

3.927 ± 0.414

1.849 ± 0.267

8.206 ± 0.271

6.92 ± 0.616

8.734 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.197

6.638 ± 0.384

3.645 ± 0.344

3.927 ± 0.292

5.001 ± 0.45

7.942 ± 0.406

6.286 ± 0.452

5.899 ± 0.41

0.775 ± 0.112

3.962 ± 0.466

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski