Bat polyomavirus 6b

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus; Dobsonia moluccensis polyomavirus 2

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D5ZYR9|A0A0D5ZYR9_9POLY Large T antigen OS=Bat polyomavirus 6b OX=1623689 PE=4 SV=1
MM1 pKa = 7.31GALLAVLAEE10 pKa = 4.1VFEE13 pKa = 4.8LSAATGFTVDD23 pKa = 4.99SILTGEE29 pKa = 4.88AIVADD34 pKa = 4.73EE35 pKa = 4.7LLQAYY40 pKa = 5.77TTNLVALEE48 pKa = 4.71GFTQTEE54 pKa = 4.02ALLAAGFSPEE64 pKa = 3.7AAYY67 pKa = 10.99LLTSLAPNFPEE78 pKa = 5.08AFSLLAGAEE87 pKa = 4.41SVVHH91 pKa = 6.35GSLFIGAATSAALTPYY107 pKa = 10.05SYY109 pKa = 11.18DD110 pKa = 3.4YY111 pKa = 9.21ATPIADD117 pKa = 4.24LNQHH121 pKa = 6.35LMALQVWRR129 pKa = 11.84PEE131 pKa = 3.94DD132 pKa = 3.25WEE134 pKa = 5.37DD135 pKa = 3.25IYY137 pKa = 11.36FPGVLPFARR146 pKa = 11.84FVNYY150 pKa = 9.56IDD152 pKa = 3.88PANWASNLYY161 pKa = 9.65HH162 pKa = 7.6AIGRR166 pKa = 11.84YY167 pKa = 7.35FWEE170 pKa = 4.27SAQRR174 pKa = 11.84AGTRR178 pKa = 11.84LIEE181 pKa = 3.87QEE183 pKa = 4.14VRR185 pKa = 11.84HH186 pKa = 5.78VSTDD190 pKa = 3.12LAQRR194 pKa = 11.84TVTSIAEE201 pKa = 3.97TLSYY205 pKa = 10.91YY206 pKa = 10.62FEE208 pKa = 4.03NARR211 pKa = 11.84WAVSHH216 pKa = 6.72LSSNIYY222 pKa = 10.59GGLQQYY228 pKa = 10.22YY229 pKa = 10.38SEE231 pKa = 4.96LPPLRR236 pKa = 11.84PHH238 pKa = 6.27QVRR241 pKa = 11.84ALHH244 pKa = 6.22KK245 pKa = 10.28RR246 pKa = 11.84LGEE249 pKa = 4.35KK250 pKa = 10.17IPDD253 pKa = 3.66RR254 pKa = 11.84FNLEE258 pKa = 3.96SSKK261 pKa = 11.26GSAQYY266 pKa = 10.39VDD268 pKa = 5.36KK269 pKa = 11.08YY270 pKa = 10.81DD271 pKa = 3.62SPGGARR277 pKa = 11.84QRR279 pKa = 11.84HH280 pKa = 4.9TPDD283 pKa = 2.34WMLPLILGLYY293 pKa = 10.54GDD295 pKa = 5.2ILPSWEE301 pKa = 4.11TTLEE305 pKa = 3.97EE306 pKa = 5.13LEE308 pKa = 4.51AEE310 pKa = 4.08EE311 pKa = 6.19DD312 pKa = 4.16GPQKK316 pKa = 10.48KK317 pKa = 9.57KK318 pKa = 10.26PRR320 pKa = 11.84SEE322 pKa = 3.59THH324 pKa = 4.84RR325 pKa = 11.84RR326 pKa = 11.84RR327 pKa = 11.84RR328 pKa = 11.84KK329 pKa = 9.04SQSSAA334 pKa = 2.93

Molecular weight: 37.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D5ZZ45|A0A0D5ZZ45_9POLY Small t antigen OS=Bat polyomavirus 6b OX=1623689 PE=4 SV=1
MM1 pKa = 7.69DD2 pKa = 4.33TGLSRR7 pKa = 11.84EE8 pKa = 3.95EE9 pKa = 4.02SQRR12 pKa = 11.84LLEE15 pKa = 4.72LLQLDD20 pKa = 4.16PEE22 pKa = 5.23HH23 pKa = 6.43YY24 pKa = 11.09GNWQLMRR31 pKa = 11.84KK32 pKa = 9.27SFLRR36 pKa = 11.84MCKK39 pKa = 9.6IMHH42 pKa = 7.06PDD44 pKa = 2.89KK45 pKa = 11.26GGNPEE50 pKa = 3.9AAKK53 pKa = 10.54EE54 pKa = 4.06LITLYY59 pKa = 10.81KK60 pKa = 10.42KK61 pKa = 10.91LEE63 pKa = 4.14NNISSLNPEE72 pKa = 3.94EE73 pKa = 5.31CFTTSQVEE81 pKa = 3.91KK82 pKa = 11.06SNFFLYY88 pKa = 10.25IKK90 pKa = 9.97DD91 pKa = 3.3WKK93 pKa = 8.56EE94 pKa = 4.02CNMGLKK100 pKa = 10.12PCVCIFCLTRR110 pKa = 11.84KK111 pKa = 7.77NHH113 pKa = 6.86KK114 pKa = 8.43EE115 pKa = 3.73RR116 pKa = 11.84KK117 pKa = 8.27NKK119 pKa = 9.31NLIWGKK125 pKa = 9.45CYY127 pKa = 10.57CFACYY132 pKa = 8.42CTWFGLEE139 pKa = 4.12WCWFTWLTWRR149 pKa = 11.84NIIAEE154 pKa = 4.42TPYY157 pKa = 10.65HH158 pKa = 6.47ALNLL162 pKa = 3.95

Molecular weight: 19.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1517

162

652

379.3

43.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.262 ± 1.518

2.373 ± 0.85

4.285 ± 0.358

7.449 ± 0.321

4.417 ± 0.382

5.735 ± 1.016

2.175 ± 0.259

4.944 ± 0.325

6.394 ± 1.242

10.481 ± 0.913

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.333

5.339 ± 0.683

5.735 ± 0.409

4.548 ± 0.375

4.417 ± 0.36

6.658 ± 0.452

5.603 ± 0.128

5.405 ± 1.039

1.582 ± 0.602

4.021 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski