Cowpea mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Gammacarmovirus

Average proteome isoelectric point is 8.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q89761|Q89761_9TOMB Capsid protein OS=Cowpea mottle virus OX=12627 PE=3 SV=1
MM1 pKa = 7.61INHH4 pKa = 6.6FAAITGVILLLWLIRR19 pKa = 11.84SHH21 pKa = 7.26SILTSIYY28 pKa = 8.57EE29 pKa = 4.04QNTPRR34 pKa = 11.84VITYY38 pKa = 11.05ANLLLLLIAYY48 pKa = 8.75CYY50 pKa = 10.14FCSTQSVSYY59 pKa = 9.97VVSHH63 pKa = 6.15SEE65 pKa = 3.62PVVNSKK71 pKa = 9.6VVYY74 pKa = 9.91ISVGTSPPSLQVSEE88 pKa = 4.39

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q89922|Q89922_9TOMB Movement protein OS=Cowpea mottle virus OX=12627 PE=4 SV=1
MM1 pKa = 6.96EE2 pKa = 4.35THH4 pKa = 6.38SPSPSSLRR12 pKa = 11.84KK13 pKa = 8.35PPHH16 pKa = 6.45RR17 pKa = 11.84GVPHH21 pKa = 5.07YY22 pKa = 10.68RR23 pKa = 11.84RR24 pKa = 11.84IKK26 pKa = 9.31RR27 pKa = 11.84SGWTMQTQTPSSPSHH42 pKa = 6.26EE43 pKa = 4.2PHH45 pKa = 6.99GSPQGRR51 pKa = 11.84SLQQPAPPSQTGCLTMRR68 pKa = 11.84FVRR71 pKa = 11.84TSLLSSMLSYY81 pKa = 10.98GVLLCQILLSPLLISPGHH99 pKa = 5.16STQVTQQLSTGSNPSLQGMICTDD122 pKa = 3.44SFVVRR127 pKa = 11.84SYY129 pKa = 9.51THH131 pKa = 6.65LGVPLRR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84DD140 pKa = 3.36QWCWLMIRR148 pKa = 11.84TLVMSILIMSPIFSIWQVLNLVLPIALYY176 pKa = 10.06RR177 pKa = 11.84LSPISSRR184 pKa = 11.84LIATFVTTALATPSLWMLGRR204 pKa = 11.84YY205 pKa = 8.85LLLAMASKK213 pKa = 10.64RR214 pKa = 11.84ALHH217 pKa = 6.32HH218 pKa = 5.61LHH220 pKa = 6.45SGKK223 pKa = 10.44SGSPTLSNSLSRR235 pKa = 11.84NLIPLGCSGWGVPPQSVGPAFARR258 pKa = 3.8

Molecular weight: 28.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1503

69

495

250.5

27.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.786 ± 0.907

1.597 ± 0.26

3.526 ± 0.849

3.526 ± 0.897

3.26 ± 0.85

6.986 ± 0.883

2.794 ± 0.422

5.256 ± 0.496

4.258 ± 0.745

9.248 ± 1.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.361

4.99 ± 0.694

6.254 ± 0.687

4.125 ± 0.421

6.653 ± 0.55

8.782 ± 1.712

7.053 ± 1.3

7.784 ± 0.646

1.53 ± 0.25

3.327 ± 0.651
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski