Gordonia phage GTE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Emalynvirus; Gordonia virus GTE2

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8S0W8|F8S0W8_9CAUD Homing endonuclease OS=Gordonia phage GTE2 OX=981323 PE=4 SV=1
MM1 pKa = 7.44NRR3 pKa = 11.84DD4 pKa = 3.31TTDD7 pKa = 2.61GMLARR12 pKa = 11.84VVCDD16 pKa = 3.47KK17 pKa = 11.15AAGIVYY23 pKa = 10.33VNDD26 pKa = 3.58VPVDD30 pKa = 3.96GYY32 pKa = 10.56IVEE35 pKa = 5.02DD36 pKa = 4.54GIGVDD41 pKa = 5.67DD42 pKa = 4.04VANPNSIMQVVLSIFAEE59 pKa = 4.26SVEE62 pKa = 4.37MIDD65 pKa = 3.71NYY67 pKa = 10.28VPRR70 pKa = 11.84VDD72 pKa = 3.68AGSINEE78 pKa = 4.15VGNTTVQIGTHH89 pKa = 5.96AGIDD93 pKa = 3.46TDD95 pKa = 4.38AVGVCYY101 pKa = 10.12LALPTMNRR109 pKa = 11.84DD110 pKa = 3.14MAGKK114 pKa = 9.68VIGMGAA120 pKa = 3.07

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8S0S1|F8S0S1_9CAUD Uncharacterized protein OS=Gordonia phage GTE2 OX=981323 PE=4 SV=1
MM1 pKa = 7.66ANRR4 pKa = 11.84VRR6 pKa = 11.84LNRR9 pKa = 11.84KK10 pKa = 6.93GFRR13 pKa = 11.84EE14 pKa = 3.88IRR16 pKa = 11.84SAPRR20 pKa = 11.84LVAKK24 pKa = 10.0IDD26 pKa = 4.14KK27 pKa = 8.37ITSGIADD34 pKa = 3.92RR35 pKa = 11.84ANRR38 pKa = 11.84NLDD41 pKa = 3.28EE42 pKa = 5.27PGYY45 pKa = 8.8KK46 pKa = 9.35TSSVEE51 pKa = 3.8GAARR55 pKa = 11.84PQGRR59 pKa = 11.84HH60 pKa = 5.46RR61 pKa = 11.84GTVITATQEE70 pKa = 4.04AKK72 pKa = 10.38EE73 pKa = 4.19DD74 pKa = 3.41NAANHH79 pKa = 5.98TLLKK83 pKa = 9.87EE84 pKa = 4.46FYY86 pKa = 9.7RR87 pKa = 11.84ARR89 pKa = 11.84GG90 pKa = 3.37

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13644

62

1547

239.4

26.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.785 ± 0.517

0.945 ± 0.146

6.245 ± 0.419

6.127 ± 0.41

3.1 ± 0.195

8.407 ± 0.44

2.133 ± 0.189

4.639 ± 0.264

4.757 ± 0.27

8.157 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.143

3.254 ± 0.189

5.446 ± 0.251

3.599 ± 0.192

6.479 ± 0.357

6.157 ± 0.32

5.966 ± 0.264

7.087 ± 0.25

2.243 ± 0.139

2.822 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski