Pyrenophora seminiperda CCB06

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Pyrenophora; Pyrenophora seminiperda

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M7M289|A0A3M7M289_9PLEO GPI anchored serine-threonine rich OS=Pyrenophora seminiperda CCB06 OX=1302712 GN=GMOD_00008222 PE=4 SV=1
MM1 pKa = 7.42SKK3 pKa = 10.2VLSATLFASLATATMTTSAWMPGMSDD29 pKa = 3.06AQASFTASVVSVNKK43 pKa = 10.35DD44 pKa = 3.15RR45 pKa = 11.84TVLSLDD51 pKa = 3.57YY52 pKa = 10.95NVSIDD57 pKa = 3.83NADD60 pKa = 3.51AAATITVGGNTYY72 pKa = 10.86YY73 pKa = 10.98GFEE76 pKa = 4.04AAATDD81 pKa = 3.59EE82 pKa = 4.6GVSVTVSGACSRR94 pKa = 11.84ANTDD98 pKa = 3.46EE99 pKa = 4.65KK100 pKa = 11.26SATCVMTTVGLDD112 pKa = 3.35SAISAVCSGPASDD125 pKa = 6.13ADD127 pKa = 4.0LCSSISGLEE136 pKa = 3.87MAMTTTLPEE145 pKa = 4.87GYY147 pKa = 9.79FDD149 pKa = 3.79TFPIIVTAGEE159 pKa = 4.02NLLPSASAAATPSASSASATAASSGFKK186 pKa = 10.37SSQTPSPTGSASRR199 pKa = 11.84GASSSSPSQTTGSPAQATGAAPMVMAPALAGMGAALAAYY238 pKa = 8.8FLL240 pKa = 4.66

Molecular weight: 23.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M7MFK8|A0A3M7MFK8_9PLEO Gti1 Pac2 family OS=Pyrenophora seminiperda CCB06 OX=1302712 GN=GMOD_00009038 PE=3 SV=1
MM1 pKa = 7.77PNSKK5 pKa = 8.22QTSPPGFYY13 pKa = 9.65PAPTNRR19 pKa = 11.84LQPPPTIAMFITPWPQLRR37 pKa = 11.84TALRR41 pKa = 11.84RR42 pKa = 11.84PQPRR46 pKa = 11.84SRR48 pKa = 11.84GSTGRR53 pKa = 11.84GVPRR57 pKa = 11.84SSPSNSRR64 pKa = 11.84SNSSATWNN72 pKa = 3.4

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8702

0

8702

4318722

8

9355

496.3

54.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.75 ± 0.024

1.23 ± 0.01

5.588 ± 0.018

6.107 ± 0.024

3.611 ± 0.016

6.69 ± 0.022

2.51 ± 0.01

4.774 ± 0.017

5.132 ± 0.024

8.611 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.01

3.749 ± 0.014

6.18 ± 0.028

4.131 ± 0.02

6.043 ± 0.022

8.056 ± 0.031

6.223 ± 0.015

6.047 ± 0.019

1.429 ± 0.01

2.815 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski