Gibberella nygamai (Bean root rot disease fungus) (Fusarium nygamai)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta;

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K0WJ50|A0A2K0WJ50_GIBNY Uncharacterized protein OS=Gibberella nygamai OX=42673 GN=FNYG_04497 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.2NAAALLTLLPLVAAHH17 pKa = 6.66GFVKK21 pKa = 10.42SPPPRR26 pKa = 11.84KK27 pKa = 9.37PGNAFKK33 pKa = 10.35AACGEE38 pKa = 4.09QPFYY42 pKa = 10.66QQSADD47 pKa = 3.16INGNVQGIKK56 pKa = 10.15QVVGSNFDD64 pKa = 3.66AKK66 pKa = 10.7EE67 pKa = 4.01CNLWLCKK74 pKa = 10.37GFQFDD79 pKa = 4.17DD80 pKa = 3.81NKK82 pKa = 11.6DD83 pKa = 3.44NVQSYY88 pKa = 11.28KK89 pKa = 10.68LGEE92 pKa = 4.64KK93 pKa = 9.51IDD95 pKa = 3.82FDD97 pKa = 4.79VNIAAPHH104 pKa = 5.53TGYY107 pKa = 11.49ANVSVVKK114 pKa = 10.03TSTDD118 pKa = 2.94KK119 pKa = 11.05MIGEE123 pKa = 4.16PLIEE127 pKa = 4.31FEE129 pKa = 5.05NYY131 pKa = 9.16AANAGLNPNNTAFSVTLPEE150 pKa = 4.25SLGGEE155 pKa = 4.23CTKK158 pKa = 11.08AGDD161 pKa = 5.53CVLQWFWDD169 pKa = 3.95APDD172 pKa = 3.63IDD174 pKa = 4.14QTYY177 pKa = 8.91EE178 pKa = 3.82SCVDD182 pKa = 3.9FVVGAGSGSGSGSGSGSTKK201 pKa = 9.87PSSAASAAPVATSAPAAEE219 pKa = 4.91KK220 pKa = 8.85PTATTLQAVAVTSSALGPVFEE241 pKa = 5.12SVTTAVPEE249 pKa = 4.29PTATTPDD256 pKa = 3.36AGDD259 pKa = 5.65DD260 pKa = 3.81EE261 pKa = 6.38DD262 pKa = 6.47CDD264 pKa = 4.51DD265 pKa = 5.14EE266 pKa = 5.32EE267 pKa = 5.61PVDD270 pKa = 4.04QGDD273 pKa = 6.0DD274 pKa = 3.64EE275 pKa = 6.24DD276 pKa = 6.45CDD278 pKa = 4.8DD279 pKa = 5.11EE280 pKa = 5.27EE281 pKa = 4.64PTPSAAAEE289 pKa = 4.11TGDD292 pKa = 6.21DD293 pKa = 3.96EE294 pKa = 6.58DD295 pKa = 5.64CDD297 pKa = 3.75EE298 pKa = 5.29DD299 pKa = 4.36EE300 pKa = 4.72EE301 pKa = 5.64PEE303 pKa = 4.18TGDD306 pKa = 5.32DD307 pKa = 5.47DD308 pKa = 4.67EE309 pKa = 5.36EE310 pKa = 5.72CPADD314 pKa = 5.98DD315 pKa = 4.24GDD317 pKa = 5.08EE318 pKa = 4.53YY319 pKa = 11.01DD320 pKa = 4.32TQPATPSNTAQGISAAANTAAVTKK344 pKa = 7.76TQNNAYY350 pKa = 8.16PVPKK354 pKa = 8.76PTGHH358 pKa = 6.35SSSDD362 pKa = 3.31KK363 pKa = 10.36TGSGSKK369 pKa = 10.67GSNNNNNAGSNYY381 pKa = 9.91NNYY384 pKa = 9.75GNSGSNNNNAGSNTIMTSYY403 pKa = 9.19VTVSAAEE410 pKa = 3.91THH412 pKa = 5.86YY413 pKa = 10.77VTVTADD419 pKa = 3.51APACTAA425 pKa = 4.06

Molecular weight: 43.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K0WBY0|A0A2K0WBY0_GIBNY Uncharacterized protein OS=Gibberella nygamai OX=42673 GN=FNYG_06869 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTTKK40 pKa = 9.5PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGAGRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 6.46ATTTAPVHH64 pKa = 4.87HH65 pKa = 6.63HH66 pKa = 5.14QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.67GSLTRR89 pKa = 11.84QPGVKK94 pKa = 9.99AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16018

0

16018

7249913

30

9543

452.6

50.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.382 ± 0.018

1.264 ± 0.008

5.919 ± 0.013

6.276 ± 0.021

3.792 ± 0.012

6.817 ± 0.019

2.365 ± 0.008

5.137 ± 0.014

5.083 ± 0.019

8.814 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.008

3.781 ± 0.01

5.843 ± 0.022

3.991 ± 0.015

5.81 ± 0.016

7.992 ± 0.02

5.984 ± 0.022

6.126 ± 0.014

1.56 ± 0.007

2.823 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski