Lactobacillus phage LR2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KCM2|A0A3G2KCM2_9CAUD Putative tail tube OS=Lactobacillus phage LR2 OX=2419582 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 3.6EE4 pKa = 4.44TPSLDD9 pKa = 3.37KK10 pKa = 10.86LVPRR14 pKa = 11.84VRR16 pKa = 11.84EE17 pKa = 3.89MLYY20 pKa = 10.78LDD22 pKa = 5.61DD23 pKa = 6.92DD24 pKa = 5.37SDD26 pKa = 4.18DD27 pKa = 3.78QLLNSYY33 pKa = 9.71VKK35 pKa = 10.4AATSFIHH42 pKa = 6.06NAIGEE47 pKa = 4.44DD48 pKa = 3.61VNGFYY53 pKa = 10.6DD54 pKa = 3.72DD55 pKa = 3.96SRR57 pKa = 11.84VVSLVDD63 pKa = 3.15IAVMSLAGTYY73 pKa = 7.99YY74 pKa = 10.39QNRR77 pKa = 11.84LALSDD82 pKa = 3.66TQTYY86 pKa = 9.67PIDD89 pKa = 3.6LTVNSIIGQLRR100 pKa = 11.84GLRR103 pKa = 11.84NSYY106 pKa = 10.81DD107 pKa = 3.52EE108 pKa = 5.39EE109 pKa = 4.48SDD111 pKa = 3.68KK112 pKa = 11.78NEE114 pKa = 4.22TSDD117 pKa = 4.7QPAEE121 pKa = 4.12SS122 pKa = 3.69

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KCP3|A0A3G2KCP3_9CAUD Putative lipoprotein OS=Lactobacillus phage LR2 OX=2419582 PE=4 SV=1
MM1 pKa = 7.25QDD3 pKa = 3.57KK4 pKa = 10.59IVVSSFWATVLVIGTLLSPIVTIILNRR31 pKa = 11.84IFDD34 pKa = 4.53LVAKK38 pKa = 10.22RR39 pKa = 11.84LNNQHH44 pKa = 5.84EE45 pKa = 4.59RR46 pKa = 11.84YY47 pKa = 9.33EE48 pKa = 4.62SYY50 pKa = 10.65NSRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.83LLEE59 pKa = 3.77QYY61 pKa = 10.87LVGLVEE67 pKa = 4.53AEE69 pKa = 3.74RR70 pKa = 11.84AEE72 pKa = 4.28TPEE75 pKa = 3.52EE76 pKa = 3.99RR77 pKa = 11.84KK78 pKa = 9.69NLKK81 pKa = 10.19LIEE84 pKa = 4.03VSYY87 pKa = 11.16LLIPYY92 pKa = 8.88LPNYY96 pKa = 7.53MQEE99 pKa = 4.0TFQRR103 pKa = 11.84YY104 pKa = 8.58ALEE107 pKa = 4.22AANRR111 pKa = 11.84KK112 pKa = 8.96HH113 pKa = 6.41DD114 pKa = 4.18AKK116 pKa = 11.31SNADD120 pKa = 3.62FQTVTIFIKK129 pKa = 10.59EE130 pKa = 4.03LLDD133 pKa = 3.36PRR135 pKa = 11.84GRR137 pKa = 11.84RR138 pKa = 11.84WIYY141 pKa = 7.99STKK144 pKa = 8.8HH145 pKa = 6.1KK146 pKa = 10.35KK147 pKa = 9.2WIWKK151 pKa = 9.55RR152 pKa = 11.84PNKK155 pKa = 10.16ALSCFRR161 pKa = 11.84QWRR164 pKa = 11.84SKK166 pKa = 8.84WKK168 pKa = 10.26

Molecular weight: 20.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11454

42

1359

279.4

31.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.29 ± 0.347

0.445 ± 0.11

7.098 ± 0.349

5.064 ± 0.261

3.405 ± 0.183

6.207 ± 0.32

1.912 ± 0.169

6.19 ± 0.25

8.015 ± 0.428

7.683 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.187

6.923 ± 0.303

3.047 ± 0.34

5.753 ± 0.263

3.71 ± 0.332

7.133 ± 0.383

6.426 ± 0.364

5.823 ± 0.263

1.353 ± 0.127

3.885 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski