Paracoccus phage vB_PkoS_Pkon1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R832|A0A3Q9R832_9CAUD Cro-like protein OS=Paracoccus phage vB_PkoS_Pkon1 OX=2500568 GN=pkon1_p24 PE=4 SV=1
MM1 pKa = 7.69TDD3 pKa = 3.66FDD5 pKa = 4.07RR6 pKa = 11.84FQRR9 pKa = 11.84PDD11 pKa = 3.43YY12 pKa = 11.26DD13 pKa = 4.29DD14 pKa = 4.85GGSLATMIVLSVVAFIFLAIGVFIGWLVYY43 pKa = 10.47APQANALPAPCADD56 pKa = 3.45YY57 pKa = 10.61RR58 pKa = 11.84AEE60 pKa = 4.14CAAVMSEE67 pKa = 3.87GWW69 pKa = 3.12

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IJS8|A0A3T0IJS8_9CAUD Uncharacterized protein OS=Paracoccus phage vB_PkoS_Pkon1 OX=2500568 GN=pkon1_p21 PE=4 SV=1
MM1 pKa = 6.5MTWPTPLIAVAAAVHH16 pKa = 6.39RR17 pKa = 11.84LATGSVPHH25 pKa = 6.77SATQTEE31 pKa = 4.75AASGPAQITGTPPEE45 pKa = 4.11NGISRR50 pKa = 11.84RR51 pKa = 11.84ITPDD55 pKa = 2.85LTARR59 pKa = 11.84AYY61 pKa = 10.42RR62 pKa = 11.84LHH64 pKa = 6.22NRR66 pKa = 11.84KK67 pKa = 9.12KK68 pKa = 10.47DD69 pKa = 3.18AAEE72 pKa = 4.05RR73 pKa = 11.84YY74 pKa = 8.84LRR76 pKa = 11.84KK77 pKa = 9.33HH78 pKa = 6.01QILDD82 pKa = 3.2KK83 pKa = 11.12GAA85 pKa = 3.52

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14892

54

1855

188.5

20.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.92 ± 0.444

0.86 ± 0.131

6.319 ± 0.212

6.305 ± 0.4

2.807 ± 0.153

7.904 ± 0.293

1.766 ± 0.166

5.526 ± 0.279

4.163 ± 0.25

8.212 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.193

2.8 ± 0.215

4.774 ± 0.289

3.566 ± 0.196

7.333 ± 0.465

5.479 ± 0.237

5.573 ± 0.338

6.084 ± 0.303

1.84 ± 0.157

2.323 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski