Mycobacterium phage Ringer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VZT1|A0A481VZT1_9CAUD Membrane protein OS=Mycobacterium phage Ringer OX=2530144 GN=76 PE=4 SV=1
MM1 pKa = 7.35PQTVHH6 pKa = 6.12VLPVDD11 pKa = 3.73DD12 pKa = 5.94LIEE15 pKa = 6.04HH16 pKa = 6.99EE17 pKa = 6.13DD18 pKa = 3.43IGDD21 pKa = 3.93DD22 pKa = 4.01CVCGPEE28 pKa = 3.97IGPVFDD34 pKa = 5.75ADD36 pKa = 4.49GACGWVITHH45 pKa = 6.86HH46 pKa = 6.49SLDD49 pKa = 3.14GRR51 pKa = 11.84EE52 pKa = 3.86RR53 pKa = 11.84FEE55 pKa = 4.31CC56 pKa = 4.82

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W0A6|A0A481W0A6_9CAUD Uncharacterized protein OS=Mycobacterium phage Ringer OX=2530144 GN=53 PE=4 SV=1
MM1 pKa = 7.46SKK3 pKa = 9.99HH4 pKa = 6.3GYY6 pKa = 8.79RR7 pKa = 11.84HH8 pKa = 5.21GQAFVQHH15 pKa = 5.11YY16 pKa = 8.74HH17 pKa = 6.29ALEE20 pKa = 4.22LGILIPAPPGYY31 pKa = 9.62RR32 pKa = 11.84GRR34 pKa = 11.84HH35 pKa = 5.49HH36 pKa = 6.94LPDD39 pKa = 3.22VRR41 pKa = 11.84FSQEE45 pKa = 3.84LPGGTVYY52 pKa = 10.53WSVNRR57 pKa = 11.84KK58 pKa = 9.3GFFRR62 pKa = 11.84RR63 pKa = 11.84DD64 pKa = 3.34DD65 pKa = 3.87NLPSGWVQRR74 pKa = 11.84IYY76 pKa = 10.86PRR78 pKa = 11.84VATSFRR84 pKa = 11.84TAEE87 pKa = 3.85

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16118

27

943

177.1

19.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.883 ± 0.429

0.645 ± 0.107

6.421 ± 0.209

6.688 ± 0.289

3.189 ± 0.158

8.605 ± 0.466

2.041 ± 0.157

4.883 ± 0.223

4.082 ± 0.234

8.469 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.117

3.208 ± 0.148

5.41 ± 0.235

3.307 ± 0.228

6.161 ± 0.424

5.875 ± 0.242

6.589 ± 0.303

7.352 ± 0.187

2.134 ± 0.129

3.003 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski