Ficedula albicollis (Collared flycatcher) (Muscicapa albicollis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15922 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3JRH0|U3JRH0_FICAL Y+L amino acid transporter 2-like OS=Ficedula albicollis OX=59894 GN=LOC101820209 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84SVLVLVCLLGMACAFSVKK20 pKa = 9.41SWLRR24 pKa = 11.84RR25 pKa = 11.84PKK27 pKa = 9.6WDD29 pKa = 3.29NSEE32 pKa = 3.91EE33 pKa = 4.02NAVFKK38 pKa = 10.85SRR40 pKa = 11.84HH41 pKa = 3.56RR42 pKa = 11.84HH43 pKa = 3.79YY44 pKa = 10.46LYY46 pKa = 10.28RR47 pKa = 11.84YY48 pKa = 8.04IYY50 pKa = 9.83VYY52 pKa = 10.04PPQRR56 pKa = 11.84RR57 pKa = 11.84YY58 pKa = 9.98QGSDD62 pKa = 2.81SSEE65 pKa = 4.14EE66 pKa = 4.0EE67 pKa = 4.01GDD69 pKa = 3.61GSEE72 pKa = 4.19EE73 pKa = 4.23EE74 pKa = 4.34EE75 pKa = 4.55GGEE78 pKa = 3.91PFYY81 pKa = 11.21AGTKK85 pKa = 9.38AAGHH89 pKa = 6.6PAGAAPGDD97 pKa = 4.06CQDD100 pKa = 3.68ALGGYY105 pKa = 9.12VSSPQQGKK113 pKa = 9.64DD114 pKa = 3.26CQGTLKK120 pKa = 10.95GIRR123 pKa = 11.84QRR125 pKa = 11.84IASKK129 pKa = 11.33GEE131 pKa = 3.73DD132 pKa = 3.84SEE134 pKa = 6.25NEE136 pKa = 3.76DD137 pKa = 2.97SDD139 pKa = 4.01EE140 pKa = 4.43NEE142 pKa = 3.98EE143 pKa = 4.09EE144 pKa = 4.21EE145 pKa = 4.32EE146 pKa = 4.18EE147 pKa = 4.28EE148 pKa = 4.17EE149 pKa = 4.53EE150 pKa = 4.79EE151 pKa = 4.81EE152 pKa = 4.69VDD154 pKa = 3.87EE155 pKa = 5.51NEE157 pKa = 4.04NGVNGTSTNTTEE169 pKa = 5.28GIGPHH174 pKa = 6.52GNGTVAAEE182 pKa = 4.19EE183 pKa = 4.62GTGEE187 pKa = 4.02AEE189 pKa = 4.02EE190 pKa = 4.37EE191 pKa = 4.19EE192 pKa = 4.39EE193 pKa = 4.18EE194 pKa = 4.26EE195 pKa = 4.2EE196 pKa = 4.21EE197 pKa = 4.21EE198 pKa = 4.21EE199 pKa = 4.21EE200 pKa = 4.21EE201 pKa = 4.21EE202 pKa = 4.21EE203 pKa = 4.26EE204 pKa = 4.25EE205 pKa = 4.47EE206 pKa = 4.49EE207 pKa = 4.48EE208 pKa = 4.1EE209 pKa = 4.6TEE211 pKa = 4.06ATTIPSTTNDD221 pKa = 3.34EE222 pKa = 4.63GLTQATTMDD231 pKa = 4.21DD232 pKa = 3.86GGPTDD237 pKa = 3.76ATTDD241 pKa = 3.2ATTDD245 pKa = 2.97ATTAGEE251 pKa = 3.89LWEE254 pKa = 4.23YY255 pKa = 11.2DD256 pKa = 3.32VTARR260 pKa = 11.84EE261 pKa = 4.01HH262 pKa = 6.34SRR264 pKa = 11.84GEE266 pKa = 4.05EE267 pKa = 4.28GTTEE271 pKa = 3.96PGYY274 pKa = 10.7EE275 pKa = 4.11EE276 pKa = 3.64QDD278 pKa = 2.98EE279 pKa = 4.48YY280 pKa = 11.93ARR282 pKa = 11.84GDD284 pKa = 3.38SYY286 pKa = 11.26RR287 pKa = 11.84AYY289 pKa = 9.36EE290 pKa = 4.68DD291 pKa = 3.56EE292 pKa = 3.82YY293 pKa = 11.36GYY295 pKa = 11.4YY296 pKa = 9.75KK297 pKa = 10.73GHH299 pKa = 6.85GYY301 pKa = 10.44DD302 pKa = 4.46VYY304 pKa = 11.29GQDD307 pKa = 3.68YY308 pKa = 10.48YY309 pKa = 11.86YY310 pKa = 11.11SQQ312 pKa = 3.52

Molecular weight: 34.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3KJR4|U3KJR4_FICAL Splicing factor 3B subunit 2-like OS=Ficedula albicollis OX=59894 GN=SF3B2 PE=4 SV=1
PP1 pKa = 7.55LPRR4 pKa = 11.84APHH7 pKa = 4.95TAARR11 pKa = 11.84APHH14 pKa = 6.27AAPRR18 pKa = 11.84APHH21 pKa = 6.2AAPRR25 pKa = 11.84APHH28 pKa = 6.2AAPRR32 pKa = 11.84APHH35 pKa = 6.2AAPRR39 pKa = 11.84APHH42 pKa = 6.2AAPRR46 pKa = 11.84APHH49 pKa = 6.2AAPRR53 pKa = 11.84APHH56 pKa = 6.2AAPRR60 pKa = 11.84APHH63 pKa = 6.2AAPRR67 pKa = 11.84APHH70 pKa = 6.2AAPRR74 pKa = 11.84APHH77 pKa = 6.2AAPRR81 pKa = 11.84APHH84 pKa = 6.55RR85 pKa = 11.84PP86 pKa = 3.49

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15289

633

15922

8677355

19

19836

545.0

60.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.844 ± 0.018

2.329 ± 0.02

4.866 ± 0.016

7.135 ± 0.027

3.699 ± 0.016

6.558 ± 0.034

2.534 ± 0.009

4.544 ± 0.015

6.013 ± 0.03

9.677 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.01

3.76 ± 0.014

6.115 ± 0.044

4.711 ± 0.02

5.462 ± 0.017

8.333 ± 0.029

5.179 ± 0.015

6.144 ± 0.018

1.192 ± 0.008

2.75 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski