Candidatus Gullanella endobia

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Gullanella

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143WR90|A0A143WR90_9ENTR 30S ribosomal protein S14 OS=Candidatus Gullanella endobia OX=1070130 GN=rpsN PE=3 SV=1
MM1 pKa = 7.64FDD3 pKa = 3.14VLIYY7 pKa = 10.87LFEE10 pKa = 4.21TYY12 pKa = 9.69IYY14 pKa = 10.94NEE16 pKa = 4.41LEE18 pKa = 3.98VCIDD22 pKa = 3.49QDD24 pKa = 4.67KK25 pKa = 9.68LTDD28 pKa = 4.26DD29 pKa = 3.83LTQAGFHH36 pKa = 6.32QDD38 pKa = 4.13DD39 pKa = 4.55IFNALNWLEE48 pKa = 4.34KK49 pKa = 10.84LIDD52 pKa = 4.06LQDD55 pKa = 4.6GIDD58 pKa = 3.31RR59 pKa = 11.84TFILHH64 pKa = 6.61SDD66 pKa = 3.65SLTMRR71 pKa = 11.84IYY73 pKa = 10.68TDD75 pKa = 3.73EE76 pKa = 4.32EE77 pKa = 4.87SHH79 pKa = 7.5FLDD82 pKa = 3.69TDD84 pKa = 3.4CRR86 pKa = 11.84GFLLFLEE93 pKa = 4.83QIQVLNFEE101 pKa = 4.32TRR103 pKa = 11.84EE104 pKa = 3.95MVIDD108 pKa = 3.82RR109 pKa = 11.84VMALDD114 pKa = 3.22ITEE117 pKa = 4.69FNLDD121 pKa = 3.59DD122 pKa = 4.88LKK124 pKa = 10.97WIILIILFSIPGYY137 pKa = 9.29EE138 pKa = 3.82NSYY141 pKa = 11.05QKK143 pKa = 9.87MEE145 pKa = 4.11NLVFEE150 pKa = 4.55KK151 pKa = 10.95NKK153 pKa = 10.39LHH155 pKa = 6.13IHH157 pKa = 6.32

Molecular weight: 18.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WR22|A0A143WR22_9ENTR 30S ribosomal protein S19 OS=Candidatus Gullanella endobia OX=1070130 GN=rpsS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLLKK11 pKa = 10.53RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.18GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.15SGRR28 pKa = 11.84QILTHH33 pKa = 6.35RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.57GRR39 pKa = 11.84VHH41 pKa = 6.27LTVSSKK47 pKa = 11.2

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

460

0

460

149785

31

1406

325.6

36.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.762 ± 0.101

1.231 ± 0.035

4.91 ± 0.068

5.635 ± 0.109

3.968 ± 0.079

6.326 ± 0.095

2.337 ± 0.053

9.183 ± 0.093

6.38 ± 0.103

10.496 ± 0.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.052

5.241 ± 0.082

3.641 ± 0.059

3.826 ± 0.06

5.643 ± 0.078

6.224 ± 0.067

5.275 ± 0.059

6.089 ± 0.085

1.028 ± 0.048

3.305 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski