Lytechinus variegatus variable sea urchin associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RLS2|A0A0K1RLS2_9CIRC Putative replication initiation protein OS=Lytechinus variegatus variable sea urchin associated circular virus OX=1692254 PE=3 SV=1
MM1 pKa = 7.71PSFRR5 pKa = 11.84VCAKK9 pKa = 10.47NFFLTYY15 pKa = 9.45PRR17 pKa = 11.84SIDD20 pKa = 4.23LSKK23 pKa = 10.96QDD25 pKa = 4.83LFDD28 pKa = 4.34FLLSFEE34 pKa = 4.65PNYY37 pKa = 10.93LLVAEE42 pKa = 5.09EE43 pKa = 4.11KK44 pKa = 10.55HH45 pKa = 7.14ADD47 pKa = 3.83GTPHH51 pKa = 7.17LHH53 pKa = 7.38ALLCLARR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84DD63 pKa = 3.46IRR65 pKa = 11.84DD66 pKa = 3.3PRR68 pKa = 11.84HH69 pKa = 6.16FDD71 pKa = 3.33CQGYY75 pKa = 8.8HH76 pKa = 6.79CNITSTRR83 pKa = 11.84SIARR87 pKa = 11.84CIDD90 pKa = 3.24YY91 pKa = 10.59CKK93 pKa = 10.59KK94 pKa = 10.58DD95 pKa = 3.48DD96 pKa = 4.65CSPLSSGDD104 pKa = 3.38VPGRR108 pKa = 11.84DD109 pKa = 3.09GWGAICEE116 pKa = 4.35CGTRR120 pKa = 11.84EE121 pKa = 4.06DD122 pKa = 3.45FMGAVRR128 pKa = 11.84RR129 pKa = 11.84YY130 pKa = 8.68YY131 pKa = 10.14PRR133 pKa = 11.84DD134 pKa = 3.18YY135 pKa = 11.16VLNYY139 pKa = 9.54EE140 pKa = 4.52KK141 pKa = 11.02LLAYY145 pKa = 10.43ADD147 pKa = 3.37AHH149 pKa = 6.21FNANVPYY156 pKa = 9.78EE157 pKa = 4.0PQFTDD162 pKa = 4.31FVLPTEE168 pKa = 4.19VDD170 pKa = 3.48DD171 pKa = 4.39WLTDD175 pKa = 3.45NFKK178 pKa = 10.9VRR180 pKa = 11.84GEE182 pKa = 4.22NAFHH186 pKa = 6.4PTGGILIGPVVRR198 pKa = 11.84RR199 pKa = 11.84AFYY202 pKa = 9.44PLCKK206 pKa = 10.46GPP208 pKa = 3.98

Molecular weight: 23.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RLS2|A0A0K1RLS2_9CIRC Putative replication initiation protein OS=Lytechinus variegatus variable sea urchin associated circular virus OX=1692254 PE=3 SV=1
MM1 pKa = 7.51GNKK4 pKa = 9.42PLITNEE10 pKa = 3.72EE11 pKa = 4.39GQVSRR16 pKa = 11.84LYY18 pKa = 10.82APKK21 pKa = 10.24KK22 pKa = 6.22QTKK25 pKa = 9.36AGKK28 pKa = 9.9KK29 pKa = 8.58KK30 pKa = 9.69AEE32 pKa = 4.0RR33 pKa = 11.84YY34 pKa = 9.59KK35 pKa = 10.72RR36 pKa = 11.84FANKK40 pKa = 9.55VEE42 pKa = 4.09KK43 pKa = 10.64VINASLYY50 pKa = 8.86EE51 pKa = 3.74GRR53 pKa = 11.84XIHH56 pKa = 7.1RR57 pKa = 11.84SAIGSAVSSGDD68 pKa = 3.22AGKK71 pKa = 10.32QGLTSLMLFSAAGATNEE88 pKa = 4.65DD89 pKa = 4.49DD90 pKa = 3.56IEE92 pKa = 5.25KK93 pKa = 10.41IFAAEE98 pKa = 4.07GLTADD103 pKa = 4.27SDD105 pKa = 4.19EE106 pKa = 5.12IYY108 pKa = 10.91LKK110 pKa = 10.49SGYY113 pKa = 10.77LNATFKK119 pKa = 11.28CNTANVTGIVDD130 pKa = 4.0IYY132 pKa = 11.38QMEE135 pKa = 4.62SKK137 pKa = 10.63SAISVFNVKK146 pKa = 8.59DD147 pKa = 3.26TALPAFVTSVNDD159 pKa = 3.22QLAAQASATSTLTVTDD175 pKa = 4.49PGWTPFCNGSVGQHH189 pKa = 5.73FKK191 pKa = 10.84IKK193 pKa = 10.29DD194 pKa = 3.28HH195 pKa = 6.24TRR197 pKa = 11.84FICRR201 pKa = 11.84PGEE204 pKa = 3.97HH205 pKa = 6.19HH206 pKa = 6.72TFTYY210 pKa = 10.03RR211 pKa = 11.84KK212 pKa = 8.85KK213 pKa = 10.37ANRR216 pKa = 11.84SVKK219 pKa = 10.59LKK221 pKa = 10.46DD222 pKa = 3.61VKK224 pKa = 10.46EE225 pKa = 4.01LLMMPHH231 pKa = 6.52LTVGFLIILSGRR243 pKa = 11.84DD244 pKa = 3.51EE245 pKa = 4.59TVSTATVAGHH255 pKa = 6.3YY256 pKa = 7.34PTQTFDD262 pKa = 3.91FQWSRR267 pKa = 11.84TYY269 pKa = 9.83WYY271 pKa = 10.68NRR273 pKa = 11.84VHH275 pKa = 6.72ANVGFGGEE283 pKa = 3.89ISS285 pKa = 3.3

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

493

208

285

246.5

27.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.316 ± 0.941

2.637 ± 1.289

6.491 ± 1.57

4.665 ± 0.201

5.68 ± 0.624

7.099 ± 0.504

3.043 ± 0.192

4.665 ± 0.486

6.288 ± 1.45

7.505 ± 0.967

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.42 ± 0.272

4.462 ± 0.366

4.665 ± 1.512

2.434 ± 0.589

5.882 ± 1.36

6.288 ± 1.164

6.897 ± 1.526

6.085 ± 0.473

1.014 ± 0.031

4.26 ± 0.611
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski