Rhodoferax sp. IMCC26218

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Rhodoferax; unclassified Rhodoferax

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E1R763|A0A3E1R763_9BURK Transglutaminase OS=Rhodoferax sp. IMCC26218 OX=2184758 GN=DIC66_20210 PE=4 SV=1
MM1 pKa = 7.38PVSGRR6 pKa = 11.84YY7 pKa = 9.37LLINASISNDD17 pKa = 3.56RR18 pKa = 11.84IAGADD23 pKa = 3.8PEE25 pKa = 4.64DD26 pKa = 4.21LLVPGLYY33 pKa = 10.14KK34 pKa = 10.84AFVPDD39 pKa = 4.34DD40 pKa = 3.98LSIGLAARR48 pKa = 11.84CAFEE52 pKa = 5.15AFNCVVPISNLDD64 pKa = 3.87TIEE67 pKa = 4.11LAVWDD72 pKa = 4.72PDD74 pKa = 3.18SDD76 pKa = 3.89AVIEE80 pKa = 4.34IDD82 pKa = 4.87FEE84 pKa = 4.57TEE86 pKa = 3.27IDD88 pKa = 4.2SMEE91 pKa = 4.53LGNRR95 pKa = 11.84CLGFEE100 pKa = 4.17QYY102 pKa = 11.44AVTSYY107 pKa = 11.01SINPRR112 pKa = 11.84PYY114 pKa = 9.07PMEE117 pKa = 4.0RR118 pKa = 11.84LFPP121 pKa = 4.76

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E1RAT1|A0A3E1RAT1_9BURK LPS export ABC transporter periplasmic protein LptC OS=Rhodoferax sp. IMCC26218 OX=2184758 GN=lptC PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4436

0

4436

1484475

37

3434

334.6

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.486 ± 0.051

1.031 ± 0.015

5.098 ± 0.026

4.991 ± 0.032

3.516 ± 0.024

8.129 ± 0.045

2.293 ± 0.021

4.359 ± 0.028

3.628 ± 0.032

10.957 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.019

2.902 ± 0.031

4.859 ± 0.029

4.516 ± 0.037

5.957 ± 0.036

5.909 ± 0.028

5.362 ± 0.038

7.679 ± 0.029

1.446 ± 0.017

2.259 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski