Gordonia phage Tiamoceli

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CSG1|A0A411CSG1_9CAUD Uncharacterized protein OS=Gordonia phage Tiamoceli OX=2510508 GN=41 PE=4 SV=1
MM1 pKa = 7.54TSPPGGGTPIVPDD14 pKa = 3.62KK15 pKa = 11.06GYY17 pKa = 8.24TAQTIYY23 pKa = 10.74QLQNVDD29 pKa = 3.54PDD31 pKa = 3.92ALGAEE36 pKa = 4.47ANLDD40 pKa = 3.39LWEE43 pKa = 4.1MLEE46 pKa = 3.95NFRR49 pKa = 11.84NKK51 pKa = 10.5LLTDD55 pKa = 4.12LLGGFASVPIAILDD69 pKa = 4.5GIGDD73 pKa = 4.34VISAITGILNGDD85 pKa = 3.68FDD87 pKa = 6.02DD88 pKa = 4.15LHH90 pKa = 6.7EE91 pKa = 4.28WAQNVIDD98 pKa = 4.6GFGNLLDD105 pKa = 4.57ALQGNYY111 pKa = 9.45TGTDD115 pKa = 3.03AFLLQIQTWAGDD127 pKa = 3.59LFAWVQDD134 pKa = 3.58VADD137 pKa = 4.9FVQNLLDD144 pKa = 4.95AILRR148 pKa = 11.84GIRR151 pKa = 11.84GIPVVGGAIADD162 pKa = 4.14VISDD166 pKa = 3.73LTGLNTKK173 pKa = 8.22ATNALSTANSAAADD187 pKa = 3.42AAAAQVAINDD197 pKa = 4.34LSADD201 pKa = 3.54MAALQVTQVYY211 pKa = 10.05EE212 pKa = 4.17SLSQHH217 pKa = 6.94DD218 pKa = 3.7IVSFPRR224 pKa = 11.84YY225 pKa = 7.89TLGLGADD232 pKa = 4.09GTTGSAGSMSCADD245 pKa = 3.75ASHH248 pKa = 5.99SHH250 pKa = 6.33SSHH253 pKa = 5.18SHH255 pKa = 4.71SVGHH259 pKa = 6.16EE260 pKa = 3.92MPTANPGKK268 pKa = 10.17GVVGYY273 pKa = 10.78VPLFVNRR280 pKa = 11.84FCRR283 pKa = 11.84PRR285 pKa = 11.84RR286 pKa = 11.84LKK288 pKa = 10.88LITGSTGWSIFSIDD302 pKa = 3.32YY303 pKa = 9.29WYY305 pKa = 11.23VALCVYY311 pKa = 10.29NPNTGNVEE319 pKa = 4.2KK320 pKa = 10.78VWDD323 pKa = 4.22GGDD326 pKa = 3.2QKK328 pKa = 11.65GAITTAAKK336 pKa = 10.29LHH338 pKa = 6.16AFDD341 pKa = 4.97MGTLNDD347 pKa = 3.89VSPGTILFGAQLQNAGLIVNTRR369 pKa = 11.84PIAALWQPGLQDD381 pKa = 3.77PSAEE385 pKa = 4.31LLTAPLYY392 pKa = 10.48NLSGQSSIPSSVPLSSLTPNNDD414 pKa = 3.09TLPWMGVGVDD424 pKa = 3.85PVV426 pKa = 3.09

Molecular weight: 44.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CSI3|A0A411CSI3_9CAUD Uncharacterized protein OS=Gordonia phage Tiamoceli OX=2510508 GN=74 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 4.33KK4 pKa = 10.64LHH6 pKa = 6.49DD7 pKa = 4.07LRR9 pKa = 11.84SFAEE13 pKa = 4.27VAGVKK18 pKa = 8.49YY19 pKa = 7.12TTMRR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 9.56HH26 pKa = 5.45ATATKK31 pKa = 10.21RR32 pKa = 11.84RR33 pKa = 11.84AEE35 pKa = 3.96AAADD39 pKa = 3.85PEE41 pKa = 4.68VKK43 pKa = 10.5LPAWLIPPPDD53 pKa = 3.87DD54 pKa = 4.52RR55 pKa = 11.84VGQSPVWRR63 pKa = 11.84DD64 pKa = 3.03RR65 pKa = 11.84TVRR68 pKa = 11.84KK69 pKa = 9.15WIEE72 pKa = 3.77SRR74 pKa = 11.84PRR76 pKa = 11.84AATHH80 pKa = 5.72ATTT83 pKa = 4.42

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

17862

47

1705

223.3

24.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.658 ± 0.408

0.795 ± 0.105

7.272 ± 0.419

5.682 ± 0.353

2.581 ± 0.201

8.958 ± 0.375

1.814 ± 0.165

4.496 ± 0.19

2.9 ± 0.214

7.687 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.106

2.794 ± 0.176

5.985 ± 0.259

3.79 ± 0.146

6.931 ± 0.394

4.938 ± 0.278

7.16 ± 0.284

7.172 ± 0.219

2.155 ± 0.152

2.323 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski