Listeria phage A118 (Bacteriophage A118)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9T196|Q9T196_BPA18 Gp28 OS=Listeria phage A118 OX=40521 PE=4 SV=1
MM1 pKa = 7.79TITTAQKK8 pKa = 10.34RR9 pKa = 11.84YY10 pKa = 8.44YY11 pKa = 10.68DD12 pKa = 3.41AMNEE16 pKa = 3.86FEE18 pKa = 5.52AIISKK23 pKa = 9.84EE24 pKa = 4.01LEE26 pKa = 3.76QTPAFSQDD34 pKa = 3.38LLNDD38 pKa = 3.16SDD40 pKa = 4.44YY41 pKa = 11.78LVITKK46 pKa = 10.51NEE48 pKa = 3.81AYY50 pKa = 10.07AVALCLLDD58 pKa = 5.28DD59 pKa = 5.1DD60 pKa = 5.67KK61 pKa = 11.94LYY63 pKa = 11.1LDD65 pKa = 3.85EE66 pKa = 4.66TLVHH70 pKa = 5.36STRR73 pKa = 11.84LNIEE77 pKa = 4.04DD78 pKa = 3.36EE79 pKa = 4.4TYY81 pKa = 10.81YY82 pKa = 10.56INFVVTNEE90 pKa = 4.63DD91 pKa = 3.7DD92 pKa = 4.32FKK94 pKa = 11.52LATDD98 pKa = 3.66EE99 pKa = 5.57DD100 pKa = 4.53KK101 pKa = 11.17EE102 pKa = 4.24KK103 pKa = 10.64HH104 pKa = 6.48DD105 pKa = 3.75KK106 pKa = 10.76QEE108 pKa = 4.07VIIKK112 pKa = 10.36SEE114 pKa = 4.09FNN116 pKa = 3.31

Molecular weight: 13.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9T152|Q9T152_BPA18 Gp68 OS=Listeria phage A118 OX=40521 PE=4 SV=1
MM1 pKa = 6.99TSYY4 pKa = 11.05YY5 pKa = 10.42YY6 pKa = 10.61SRR8 pKa = 11.84SLANVNKK15 pKa = 10.36LADD18 pKa = 3.72NTKK21 pKa = 9.71AAARR25 pKa = 11.84KK26 pKa = 9.34LLDD29 pKa = 3.23WSEE32 pKa = 4.69SNGIEE37 pKa = 3.82VLIYY41 pKa = 8.1EE42 pKa = 4.8TIRR45 pKa = 11.84TKK47 pKa = 10.1EE48 pKa = 3.66QQAANVNSGASQTMRR63 pKa = 11.84SYY65 pKa = 11.3HH66 pKa = 6.34LVGQALDD73 pKa = 3.54FVMAKK78 pKa = 10.26GKK80 pKa = 8.01TVDD83 pKa = 2.84WGAYY87 pKa = 9.86RR88 pKa = 11.84SDD90 pKa = 3.35KK91 pKa = 10.84GKK93 pKa = 10.96KK94 pKa = 9.09FVAKK98 pKa = 10.19AKK100 pKa = 10.6SLGFEE105 pKa = 4.22WGGDD109 pKa = 2.83WSGFVDD115 pKa = 4.56NPHH118 pKa = 6.62LQFNYY123 pKa = 10.07KK124 pKa = 10.47GYY126 pKa = 8.51GTDD129 pKa = 3.18TFGKK133 pKa = 10.16GASTSNSSKK142 pKa = 10.55PSADD146 pKa = 3.2TNTNSLGLVDD156 pKa = 5.26YY157 pKa = 10.46MNLNKK162 pKa = 10.11LDD164 pKa = 3.72SSFANRR170 pKa = 11.84KK171 pKa = 8.96KK172 pKa = 10.61LATSYY177 pKa = 10.89GIKK180 pKa = 10.18NYY182 pKa = 10.82SGTATQNTTLLAKK195 pKa = 10.48LKK197 pKa = 10.2AGKK200 pKa = 8.64PHH202 pKa = 6.52TPASKK207 pKa = 8.63NTYY210 pKa = 7.27YY211 pKa = 10.57TEE213 pKa = 3.99NPRR216 pKa = 11.84KK217 pKa = 10.22VKK219 pKa = 9.74TLVQCDD225 pKa = 4.01LYY227 pKa = 11.32KK228 pKa = 10.97SVDD231 pKa = 4.09FTTKK235 pKa = 10.16NQTGGTFPPGTVFTISGMGKK255 pKa = 7.82TKK257 pKa = 10.68GGTPRR262 pKa = 11.84LKK264 pKa = 9.94TKK266 pKa = 10.16SGYY269 pKa = 10.73YY270 pKa = 8.86LTANTKK276 pKa = 8.89FVKK279 pKa = 10.46KK280 pKa = 10.26II281 pKa = 3.37

Molecular weight: 30.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

1

73

12936

21

1794

177.2

19.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 0.97

0.688 ± 0.122

5.821 ± 0.285

7.893 ± 0.625

3.857 ± 0.226

5.821 ± 0.427

1.221 ± 0.169

7.089 ± 0.308

9.176 ± 0.406

8.31 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.125

6.339 ± 0.302

2.273 ± 0.189

3.749 ± 0.18

3.486 ± 0.317

6.625 ± 0.476

6.099 ± 0.269

6.285 ± 0.227

1.051 ± 0.159

3.958 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski