Aeromonas phage pAh6-C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Nefertitivirinae; Pahsextavirus; Aeromonas virus pAh6C

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076G5M3|A0A076G5M3_9CAUD Uncharacterized protein OS=Aeromonas phage pAh6-C OX=1505227 GN=AH6C_048 PE=4 SV=1
MM1 pKa = 7.88ISQEE5 pKa = 4.11KK6 pKa = 9.55LAKK9 pKa = 8.91LTNTAEE15 pKa = 4.21LAKK18 pKa = 10.14KK19 pKa = 9.94VCAIVNTLAVGEE31 pKa = 4.13AWVAGGIVRR40 pKa = 11.84DD41 pKa = 3.79LWAAQTQGVDD51 pKa = 3.59LEE53 pKa = 4.53GSGDD57 pKa = 3.57IDD59 pKa = 3.63VLLINCPNDD68 pKa = 3.72DD69 pKa = 4.48LNQLGAFISAALDD82 pKa = 3.17IKK84 pKa = 10.62GIKK87 pKa = 9.62AYY89 pKa = 10.16CSRR92 pKa = 11.84VDD94 pKa = 3.41YY95 pKa = 10.78SYY97 pKa = 11.34EE98 pKa = 3.84ATEE101 pKa = 5.22DD102 pKa = 3.83YY103 pKa = 10.99PDD105 pKa = 4.69ADD107 pKa = 3.66DD108 pKa = 4.23RR109 pKa = 11.84LYY111 pKa = 11.48GVMQFSIYY119 pKa = 10.58SSGAADD125 pKa = 3.65ASTTEE130 pKa = 3.85MDD132 pKa = 3.09ILVYY136 pKa = 10.16EE137 pKa = 4.65DD138 pKa = 3.92RR139 pKa = 11.84FEE141 pKa = 4.16TLEE144 pKa = 4.46DD145 pKa = 3.56VMDD148 pKa = 4.34TFNCNINKK156 pKa = 9.1FWFDD160 pKa = 3.43EE161 pKa = 4.35EE162 pKa = 5.61GNGNTTFSPEE172 pKa = 3.48QPFRR176 pKa = 11.84YY177 pKa = 9.51RR178 pKa = 11.84EE179 pKa = 3.72GDD181 pKa = 3.28RR182 pKa = 11.84EE183 pKa = 4.24KK184 pKa = 11.33NEE186 pKa = 3.6EE187 pKa = 4.1RR188 pKa = 11.84YY189 pKa = 8.89EE190 pKa = 4.18KK191 pKa = 10.79CVMRR195 pKa = 11.84YY196 pKa = 10.35NMVARR201 pKa = 11.84AAHH204 pKa = 6.95PYY206 pKa = 9.61QRR208 pKa = 11.84LDD210 pKa = 3.46AEE212 pKa = 4.95PEE214 pKa = 3.77LGMASEE220 pKa = 4.37QVDD223 pKa = 3.59AQLPCC228 pKa = 4.88

Molecular weight: 25.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076G4E4|A0A076G4E4_9CAUD Uncharacterized protein OS=Aeromonas phage pAh6-C OX=1505227 GN=AH6C_036 PE=4 SV=1
MM1 pKa = 6.96QKK3 pKa = 10.73YY4 pKa = 7.97MKK6 pKa = 10.65LLGILMLLHH15 pKa = 7.04IFPQKK20 pKa = 10.9AMASKK25 pKa = 9.93GHH27 pKa = 6.37NYY29 pKa = 10.81DD30 pKa = 2.92NTRR33 pKa = 11.84CYY35 pKa = 10.69AQAAYY40 pKa = 10.33HH41 pKa = 5.77EE42 pKa = 4.85ARR44 pKa = 11.84SIDD47 pKa = 3.54RR48 pKa = 11.84TAMRR52 pKa = 11.84LVQSVVYY59 pKa = 10.59NRR61 pKa = 11.84VKK63 pKa = 10.16MKK65 pKa = 10.47GYY67 pKa = 9.5PRR69 pKa = 11.84DD70 pKa = 3.33ACGVVWQRR78 pKa = 11.84AQFSWTLRR86 pKa = 11.84HH87 pKa = 5.72PHH89 pKa = 6.73SIGALPVALPPADD102 pKa = 3.43GAALTEE108 pKa = 4.23ALSLARR114 pKa = 11.84SLRR117 pKa = 11.84AGDD120 pKa = 3.49WRR122 pKa = 11.84PSITADD128 pKa = 3.13HH129 pKa = 6.53FLSPDD134 pKa = 3.37VLSGAKK140 pKa = 9.04TGRR143 pKa = 11.84PRR145 pKa = 11.84FPAWARR151 pKa = 11.84CPAGQPDD158 pKa = 3.4RR159 pKa = 11.84CMSYY163 pKa = 10.58KK164 pKa = 10.34GLKK167 pKa = 10.0LLAPDD172 pKa = 3.93FTYY175 pKa = 10.7RR176 pKa = 11.84GLWFYY181 pKa = 9.92TINN184 pKa = 3.54

Molecular weight: 20.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

16206

35

843

188.4

20.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.83 ± 0.361

1.259 ± 0.106

5.985 ± 0.223

6.238 ± 0.307

3.542 ± 0.168

7.608 ± 0.262

2.203 ± 0.149

5.671 ± 0.194

5.658 ± 0.262

7.738 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.196 ± 0.13

4.289 ± 0.182

4.319 ± 0.2

4.233 ± 0.207

5.412 ± 0.213

5.837 ± 0.229

6.084 ± 0.248

7.115 ± 0.341

1.395 ± 0.098

3.388 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski