Bacillus sporothermodurans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Heyndrickxia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150L7V4|A0A150L7V4_9BACI Uncharacterized protein OS=Bacillus sporothermodurans OX=46224 GN=B4102_2865 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 5.36ACNSGSHH9 pKa = 5.18WKK11 pKa = 10.22ANKK14 pKa = 9.58DD15 pKa = 3.22AAEE18 pKa = 4.36KK19 pKa = 11.03YY20 pKa = 7.49GTGWATTAAAGYY32 pKa = 10.53VSGADD37 pKa = 3.37QYY39 pKa = 11.99AGASSVDD46 pKa = 3.44NQALFDD52 pKa = 4.54DD53 pKa = 5.17LCNPLQSLTTFKK65 pKa = 10.84QLLSGTSDD73 pKa = 3.91NSDD76 pKa = 3.29TNNNNDD82 pKa = 3.93NGNTNNTPQEE92 pKa = 4.15DD93 pKa = 4.87PYY95 pKa = 10.66QTGAEE100 pKa = 4.21FF101 pKa = 4.04

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150KLJ1|A0A150KLJ1_9BACI Uncharacterized protein OS=Bacillus sporothermodurans OX=46224 GN=B4102_3610 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPNARR10 pKa = 11.84KK11 pKa = 9.43RR12 pKa = 11.84SKK14 pKa = 10.14VHH16 pKa = 5.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4209

0

4209

1127785

37

2137

267.9

30.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.253 ± 0.043

0.728 ± 0.012

5.033 ± 0.029

7.306 ± 0.051

4.627 ± 0.038

6.539 ± 0.042

2.069 ± 0.02

8.547 ± 0.041

7.968 ± 0.046

9.53 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.02

5.006 ± 0.034

3.51 ± 0.023

3.716 ± 0.027

3.867 ± 0.029

6.08 ± 0.029

5.315 ± 0.025

6.578 ± 0.03

1.005 ± 0.014

3.643 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski