Pseudomonas phage vB_PaeS_PM105

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Beetrevirus; Pseudomonas virus PM105

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4L5C0|A0A0S4L5C0_9CAUD Putative holin protein OS=Pseudomonas phage vB_PaeS_PM105 OX=1743016 GN=PM105_23 PE=4 SV=2
MM1 pKa = 7.8ADD3 pKa = 3.55VLEE6 pKa = 4.72IDD8 pKa = 5.11CPACSTPYY16 pKa = 10.46PEE18 pKa = 4.11ITAGSAAHH26 pKa = 6.78DD27 pKa = 3.91PSLIEE32 pKa = 5.41LVITCSNCGHH42 pKa = 7.22ILNAFVSLAEE52 pKa = 4.12MSVVPNPEE60 pKa = 3.57EE61 pKa = 4.08EE62 pKa = 4.55TPHH65 pKa = 5.88GG66 pKa = 3.98

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4L561|A0A0S4L561_9CAUD Putative FAD/FMN-containing dehydrogenase OS=Pseudomonas phage vB_PaeS_PM105 OX=1743016 GN=PM105_49 PE=4 SV=1
MM1 pKa = 7.24SGARR5 pKa = 11.84IEE7 pKa = 5.24LEE9 pKa = 3.8FDD11 pKa = 3.45SQQVTQALSAAAATLRR27 pKa = 11.84SPTKK31 pKa = 10.07ILEE34 pKa = 4.21DD35 pKa = 4.55LIEE38 pKa = 4.67PLLDD42 pKa = 2.86IHH44 pKa = 6.04QARR47 pKa = 11.84FVAQRR52 pKa = 11.84APDD55 pKa = 3.88GTPWAALSPRR65 pKa = 11.84YY66 pKa = 8.25QARR69 pKa = 11.84KK70 pKa = 8.95RR71 pKa = 11.84RR72 pKa = 11.84NRR74 pKa = 11.84DD75 pKa = 3.15KK76 pKa = 10.95ILTASGDD83 pKa = 3.67LRR85 pKa = 11.84KK86 pKa = 9.64LAGQVEE92 pKa = 4.9GDD94 pKa = 3.51TLLFGTNLPYY104 pKa = 10.71GAIHH108 pKa = 5.98QFGGTIQRR116 pKa = 11.84QEE118 pKa = 4.14RR119 pKa = 11.84QSTVYY124 pKa = 10.48FRR126 pKa = 11.84MNEE129 pKa = 3.75RR130 pKa = 11.84TGEE133 pKa = 4.08VGRR136 pKa = 11.84QFVPKK141 pKa = 9.85RR142 pKa = 11.84RR143 pKa = 11.84SNFAQDD149 pKa = 3.55VRR151 pKa = 11.84IGPYY155 pKa = 9.51TITMPARR162 pKa = 11.84PWLGTSDD169 pKa = 3.55TDD171 pKa = 4.91DD172 pKa = 3.72AKK174 pKa = 11.21LLQRR178 pKa = 11.84VMSLINSTLQNN189 pKa = 3.34

Molecular weight: 21.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12689

52

1283

226.6

24.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.688 ± 0.667

0.796 ± 0.129

5.729 ± 0.18

6.494 ± 0.395

2.821 ± 0.163

7.755 ± 0.347

1.757 ± 0.227

4.271 ± 0.248

3.475 ± 0.276

9.812 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.144 ± 0.117

3.215 ± 0.23

4.91 ± 0.336

5.572 ± 0.36

7.14 ± 0.331

5.619 ± 0.292

5.414 ± 0.251

6.179 ± 0.336

1.623 ± 0.171

2.585 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski