Saccharomyces cerevisiae killer virus M1 (ScV-M1) (Saccharomyces cerevisiae virus M1)

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 4.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P01546|M11P_SCVM1 M1-1 protoxin OS=Saccharomyces cerevisiae killer virus M1 OX=12450 PE=2 SV=1
MM1 pKa = 7.48TKK3 pKa = 8.67PTQVLVRR10 pKa = 11.84SVSILFFITLLHH22 pKa = 6.4LVVALNDD29 pKa = 3.45VAGPAEE35 pKa = 4.1TAPVSLLPRR44 pKa = 11.84EE45 pKa = 4.38APWYY49 pKa = 9.68DD50 pKa = 3.92KK51 pKa = 10.62IWEE54 pKa = 4.26VKK56 pKa = 10.09DD57 pKa = 3.27WLLQRR62 pKa = 11.84ATDD65 pKa = 4.23GNWGKK70 pKa = 10.91SITWGSFVASDD81 pKa = 3.42AGVVIFGINVCKK93 pKa = 10.82NCVGEE98 pKa = 4.21RR99 pKa = 11.84KK100 pKa = 10.0DD101 pKa = 6.11DD102 pKa = 3.4ISTDD106 pKa = 3.5CGKK109 pKa = 8.45QTLALLVSIFVAVTSGHH126 pKa = 5.53HH127 pKa = 6.46LIWGGNRR134 pKa = 11.84PVSQSDD140 pKa = 3.6PNGATVARR148 pKa = 11.84RR149 pKa = 11.84DD150 pKa = 3.29ISTVADD156 pKa = 3.53GDD158 pKa = 3.87IPLDD162 pKa = 4.16FSALNDD168 pKa = 3.43ILNEE172 pKa = 4.0HH173 pKa = 7.36GISILPANASQYY185 pKa = 10.4VKK187 pKa = 10.68RR188 pKa = 11.84SDD190 pKa = 3.62TAEE193 pKa = 3.96HH194 pKa = 5.31TTSFVVTNNYY204 pKa = 8.38TSLHH208 pKa = 6.27TDD210 pKa = 4.83LIHH213 pKa = 7.0HH214 pKa = 6.78GNGTYY219 pKa = 7.36TTFTTPHH226 pKa = 6.16IPAVAKK232 pKa = 10.11RR233 pKa = 11.84YY234 pKa = 8.43VYY236 pKa = 9.89PMCEE240 pKa = 3.86HH241 pKa = 7.32GIKK244 pKa = 10.36ASYY247 pKa = 10.83CMALNDD253 pKa = 4.87AMVSANGNLYY263 pKa = 10.73GLAEE267 pKa = 4.24KK268 pKa = 10.65LFSEE272 pKa = 6.28DD273 pKa = 2.77EE274 pKa = 4.6GQWEE278 pKa = 4.1TNYY281 pKa = 10.9YY282 pKa = 9.33KK283 pKa = 10.82LYY285 pKa = 9.74WSTGQWIMSMKK296 pKa = 10.33FIEE299 pKa = 4.33EE300 pKa = 4.46SIDD303 pKa = 3.31NANNDD308 pKa = 4.21FEE310 pKa = 6.26GCDD313 pKa = 3.28TGHH316 pKa = 7.08

Molecular weight: 34.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P01546|M11P_SCVM1 M1-1 protoxin OS=Saccharomyces cerevisiae killer virus M1 OX=12450 PE=2 SV=1
MM1 pKa = 7.48TKK3 pKa = 8.67PTQVLVRR10 pKa = 11.84SVSILFFITLLHH22 pKa = 6.4LVVALNDD29 pKa = 3.45VAGPAEE35 pKa = 4.1TAPVSLLPRR44 pKa = 11.84EE45 pKa = 4.38APWYY49 pKa = 9.68DD50 pKa = 3.92KK51 pKa = 10.62IWEE54 pKa = 4.26VKK56 pKa = 10.09DD57 pKa = 3.27WLLQRR62 pKa = 11.84ATDD65 pKa = 4.23GNWGKK70 pKa = 10.91SITWGSFVASDD81 pKa = 3.42AGVVIFGINVCKK93 pKa = 10.82NCVGEE98 pKa = 4.21RR99 pKa = 11.84KK100 pKa = 10.0DD101 pKa = 6.11DD102 pKa = 3.4ISTDD106 pKa = 3.5CGKK109 pKa = 8.45QTLALLVSIFVAVTSGHH126 pKa = 5.53HH127 pKa = 6.46LIWGGNRR134 pKa = 11.84PVSQSDD140 pKa = 3.6PNGATVARR148 pKa = 11.84RR149 pKa = 11.84DD150 pKa = 3.29ISTVADD156 pKa = 3.53GDD158 pKa = 3.87IPLDD162 pKa = 4.16FSALNDD168 pKa = 3.43ILNEE172 pKa = 4.0HH173 pKa = 7.36GISILPANASQYY185 pKa = 10.4VKK187 pKa = 10.68RR188 pKa = 11.84SDD190 pKa = 3.62TAEE193 pKa = 3.96HH194 pKa = 5.31TTSFVVTNNYY204 pKa = 8.38TSLHH208 pKa = 6.27TDD210 pKa = 4.83LIHH213 pKa = 7.0HH214 pKa = 6.78GNGTYY219 pKa = 7.36TTFTTPHH226 pKa = 6.16IPAVAKK232 pKa = 10.11RR233 pKa = 11.84YY234 pKa = 8.43VYY236 pKa = 9.89PMCEE240 pKa = 3.86HH241 pKa = 7.32GIKK244 pKa = 10.36ASYY247 pKa = 10.83CMALNDD253 pKa = 4.87AMVSANGNLYY263 pKa = 10.73GLAEE267 pKa = 4.24KK268 pKa = 10.65LFSEE272 pKa = 6.28DD273 pKa = 2.77EE274 pKa = 4.6GQWEE278 pKa = 4.1TNYY281 pKa = 10.9YY282 pKa = 9.33KK283 pKa = 10.82LYY285 pKa = 9.74WSTGQWIMSMKK296 pKa = 10.33FIEE299 pKa = 4.33EE300 pKa = 4.46SIDD303 pKa = 3.31NANNDD308 pKa = 4.21FEE310 pKa = 6.26GCDD313 pKa = 3.28TGHH316 pKa = 7.08

Molecular weight: 34.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

316

316

316

316.0

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.911 ± 0.0

1.899 ± 0.0

6.646 ± 0.0

4.43 ± 0.0

3.481 ± 0.0

7.278 ± 0.0

3.481 ± 0.0

6.329 ± 0.0

4.114 ± 0.0

7.911 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.899 ± 0.0

6.013 ± 0.0

3.797 ± 0.0

2.215 ± 0.0

2.848 ± 0.0

7.595 ± 0.0

7.911 ± 0.0

7.911 ± 0.0

2.848 ± 0.0

3.481 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski