Marinobacter sp. X15-166B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified Marinobacter

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7PY70|A0A1E7PY70_9ALTE Peptide chain release factor 1 OS=Marinobacter sp. X15-166B OX=1897620 GN=prfA PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 10.51KK3 pKa = 10.24LLQGVALVSTAAVCQTASALVIDD26 pKa = 4.47ATSTDD31 pKa = 3.38GTAMANALLGSGVTISNANYY51 pKa = 9.73IGGPNQSGFFSDD63 pKa = 4.27GAAVLNIDD71 pKa = 4.41EE72 pKa = 4.59GLIMTTGDD80 pKa = 3.18ATDD83 pKa = 3.63APGPNSAGGTTTVVGGAGDD102 pKa = 4.29ADD104 pKa = 4.34LSALIGGATTNDD116 pKa = 3.42AAVLTFDD123 pKa = 4.24FTTTTGDD130 pKa = 3.47LFFNYY135 pKa = 9.06VFASEE140 pKa = 4.71EE141 pKa = 3.94YY142 pKa = 10.01NEE144 pKa = 4.35FVGTQFNDD152 pKa = 2.83VFAFFVNGVNIAEE165 pKa = 4.66APDD168 pKa = 3.96GQPVSINNVNCGNPFSGAGPNCDD191 pKa = 3.46FYY193 pKa = 11.9NNNEE197 pKa = 4.06TGVFDD202 pKa = 3.98IEE204 pKa = 4.33YY205 pKa = 10.72DD206 pKa = 3.38GFTDD210 pKa = 3.79VFTASVLGLDD220 pKa = 4.04PEE222 pKa = 4.72ATHH225 pKa = 6.34SMKK228 pKa = 10.33IAIADD233 pKa = 3.86TGDD236 pKa = 3.76SSWDD240 pKa = 3.31SAVFLQGGSFTDD252 pKa = 3.74APPPVSTVPEE262 pKa = 4.61PGSLALMLLGLGGLLANRR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84LHH284 pKa = 5.57SS285 pKa = 3.76

Molecular weight: 29.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7PY37|A0A1E7PY37_9ALTE Uncharacterized protein OS=Marinobacter sp. X15-166B OX=1897620 GN=BG841_09940 PE=4 SV=1
MM1 pKa = 7.48TSFFALQQRR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 9.56RR14 pKa = 11.84IHH16 pKa = 5.48QQWPGLRR23 pKa = 11.84PLAATGEE30 pKa = 4.12QLVNGTRR37 pKa = 11.84ASRR40 pKa = 11.84GTGGRR45 pKa = 11.84RR46 pKa = 11.84HH47 pKa = 6.54LSWLNNQLSRR57 pKa = 11.84NPLTNQRR64 pKa = 11.84NNSRR68 pKa = 11.84YY69 pKa = 9.57HH70 pKa = 5.93NKK72 pKa = 9.32QVQQRR77 pKa = 11.84RR78 pKa = 11.84MATTQRR84 pKa = 11.84VRR86 pKa = 11.84QQ87 pKa = 3.63

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3115

0

3115

999817

51

2469

321.0

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.863 ± 0.044

0.941 ± 0.014

5.576 ± 0.033

5.909 ± 0.04

3.63 ± 0.03

7.973 ± 0.04

2.337 ± 0.02

5.061 ± 0.035

3.134 ± 0.034

10.868 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.02

3.069 ± 0.028

4.789 ± 0.032

4.312 ± 0.029

6.638 ± 0.037

5.51 ± 0.027

5.437 ± 0.03

7.556 ± 0.038

1.334 ± 0.017

2.563 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski