Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Leptosphaeriaceae; Leptosphaeria; Leptosphaeria maculans species complex; Leptosphaeria maculans; Leptosphaeria maculans 'brassicae' group

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4ZQ02|E4ZQ02_LEPMJ Predicted protein OS=Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) OX=985895 GN=LEMA_uP044380.1 PE=4 SV=1
MM1 pKa = 7.72ADD3 pKa = 3.84EE4 pKa = 4.62EE5 pKa = 4.85VNLYY9 pKa = 10.85DD10 pKa = 4.43EE11 pKa = 5.94IEE13 pKa = 4.67IEE15 pKa = 4.26DD16 pKa = 4.1CFYY19 pKa = 11.51DD20 pKa = 3.82EE21 pKa = 5.15ALGIYY26 pKa = 8.35HH27 pKa = 6.94HH28 pKa = 7.3PCPCGDD34 pKa = 3.39RR35 pKa = 11.84FEE37 pKa = 5.58ILLDD41 pKa = 3.78DD42 pKa = 4.11MRR44 pKa = 11.84DD45 pKa = 3.6GEE47 pKa = 5.06DD48 pKa = 2.91IARR51 pKa = 11.84CPSCSLMIRR60 pKa = 11.84IIFDD64 pKa = 3.84PSDD67 pKa = 3.58LPEE70 pKa = 4.6NKK72 pKa = 9.8SSAPQQTAITAA83 pKa = 3.81

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4ZZ50|E4ZZ50_LEPMJ Zn(2)-C6 fungal-type domain-containing protein OS=Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) OX=985895 GN=LEMA_P109100.1 PE=4 SV=1
MM1 pKa = 7.37LRR3 pKa = 11.84NAPMFRR9 pKa = 11.84SRR11 pKa = 11.84GTRR14 pKa = 11.84GRR16 pKa = 11.84GVGLARR22 pKa = 11.84GRR24 pKa = 11.84ATVNRR29 pKa = 11.84ARR31 pKa = 11.84TSGRR35 pKa = 11.84GGPGGPGGPGGRR47 pKa = 4.0

Molecular weight: 4.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12608

0

12608

5283209

33

9743

419.0

46.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.018

1.335 ± 0.009

5.459 ± 0.016

5.97 ± 0.029

3.522 ± 0.014

6.678 ± 0.024

2.646 ± 0.011

4.703 ± 0.015

4.848 ± 0.022

8.661 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.009

3.631 ± 0.012

6.39 ± 0.027

4.197 ± 0.018

6.308 ± 0.02

8.309 ± 0.027

6.247 ± 0.017

5.916 ± 0.016

1.425 ± 0.009

2.689 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski