Methylophilaceae bacterium 11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; unclassified Methylophilaceae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Y0KJP1|Y0KJP1_9PROT Transcriptional regulator OS=Methylophilaceae bacterium 11 OX=1101195 GN=Meth11DRAFT_2075 PE=4 SV=1
MM1 pKa = 7.65SEE3 pKa = 3.82QTVASPCIGVCSMDD17 pKa = 4.6DD18 pKa = 4.19LSGLCLGCYY27 pKa = 8.37RR28 pKa = 11.84TIEE31 pKa = 4.63EE32 pKa = 4.03IQAWWDD38 pKa = 3.13MDD40 pKa = 4.11DD41 pKa = 4.71ASKK44 pKa = 11.11QSIIAQADD52 pKa = 3.54DD53 pKa = 3.59RR54 pKa = 11.84ASTLFGDD61 pKa = 4.31

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Y0KL37|Y0KL37_9PROT Uncharacterized protein OS=Methylophilaceae bacterium 11 OX=1101195 GN=Meth11DRAFT_2492 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.36GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2589

0

2589

851956

23

5481

329.1

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.025 ± 0.058

0.838 ± 0.016

5.218 ± 0.037

5.439 ± 0.051

3.84 ± 0.039

6.851 ± 0.061

2.415 ± 0.03

6.285 ± 0.04

5.261 ± 0.056

10.31 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.024

4.292 ± 0.05

4.112 ± 0.029

4.742 ± 0.042

4.534 ± 0.034

6.041 ± 0.038

5.869 ± 0.087

7.137 ± 0.039

1.196 ± 0.019

2.982 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski