Acidocella aminolytica 101 = DSM 11237

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidocella; Acidocella aminolytica

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6PLU2|A0A0D6PLU2_9PROT Iron-binding nuclear protein Pirin OS=Acidocella aminolytica 101 = DSM 11237 OX=1120923 GN=Aam_114_026 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.32PALFTIVVLCAGGALTLCVVSDD24 pKa = 4.61AAAQMAVIDD33 pKa = 4.45SANLGQNIQTAAQSVIAVEE52 pKa = 3.85QLKK55 pKa = 10.61AQLSQLEE62 pKa = 3.99QTYY65 pKa = 10.88QMFTNPTNVLGMAAGMEE82 pKa = 4.2NQSIEE87 pKa = 4.35NPMPMANAMAGLVGGAAAPSGAAASYY113 pKa = 9.52YY114 pKa = 9.31NQNHH118 pKa = 5.65VYY120 pKa = 10.7APTDD124 pKa = 3.45GSAASAQLNANGNAIANIEE143 pKa = 4.66GIASTNLAAIQQRR156 pKa = 11.84MQDD159 pKa = 3.94LPNLEE164 pKa = 4.45SDD166 pKa = 4.36LNAATSITQVDD177 pKa = 4.93AINGRR182 pKa = 11.84IAAEE186 pKa = 4.13SQFVQAQQAQAQNLQVLASEE206 pKa = 4.5QALSQQQQQKK216 pKa = 8.02EE217 pKa = 4.18QFVEE221 pKa = 4.14DD222 pKa = 3.75MTNASSDD229 pKa = 3.73YY230 pKa = 10.2SQAAAADD237 pKa = 3.75NTGQQ241 pKa = 3.59

Molecular weight: 24.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6PGD6|A0A0D6PGD6_9PROT Uncharacterized protein OS=Acidocella aminolytica 101 = DSM 11237 OX=1120923 GN=Aam_041_030 PE=4 SV=1
MM1 pKa = 7.79AIVRR5 pKa = 11.84PVNISATAWARR16 pKa = 11.84RR17 pKa = 11.84VGGTRR22 pKa = 11.84AAASTVPMQKK32 pKa = 9.99KK33 pKa = 9.32AQWQISVTIRR43 pKa = 11.84ASIRR47 pKa = 11.84KK48 pKa = 8.9SS49 pKa = 3.07

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3751

0

3751

1126875

40

2387

300.4

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.786 ± 0.071

0.926 ± 0.014

4.905 ± 0.029

5.427 ± 0.049

3.695 ± 0.031

8.544 ± 0.057

2.204 ± 0.017

5.194 ± 0.032

3.427 ± 0.03

10.781 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.021

2.889 ± 0.032

5.367 ± 0.032

3.477 ± 0.028

6.427 ± 0.052

5.365 ± 0.036

5.396 ± 0.034

7.071 ± 0.031

1.341 ± 0.016

2.264 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski