Erythrobacter sp. YT30

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A109LNU1|A0A109LNU1_9SPHN Uncharacterized protein OS=Erythrobacter sp. YT30 OX=1735012 GN=AUC45_07155 PE=3 SV=1
MM1 pKa = 7.21TAILDD6 pKa = 3.81AAGGDD11 pKa = 3.76TAIEE15 pKa = 4.01NSLLEE20 pKa = 4.87ADD22 pKa = 5.03DD23 pKa = 4.62EE24 pKa = 4.79EE25 pKa = 4.83SLQTQILGLTPEE37 pKa = 4.14YY38 pKa = 10.97SGGNFDD44 pKa = 5.5LATSSSRR51 pKa = 11.84LAAAHH56 pKa = 6.88LYY58 pKa = 10.34DD59 pKa = 4.65HH60 pKa = 6.71EE61 pKa = 4.92TMFSVSDD68 pKa = 3.24TRR70 pKa = 11.84IWVEE74 pKa = 3.81SYY76 pKa = 11.1YY77 pKa = 11.24LNGDD81 pKa = 3.66YY82 pKa = 11.09DD83 pKa = 4.3PDD85 pKa = 5.58SIAGGYY91 pKa = 9.56SLGSFGLTGGYY102 pKa = 8.73EE103 pKa = 3.79AGTPFGRR110 pKa = 11.84VGLSANISWGSNSNGTVDD128 pKa = 3.88AEE130 pKa = 4.31VDD132 pKa = 3.29ASHH135 pKa = 6.96YY136 pKa = 9.4EE137 pKa = 4.01AALHH141 pKa = 5.78WRR143 pKa = 11.84EE144 pKa = 3.98QLGGLLMFARR154 pKa = 11.84GSFAYY159 pKa = 9.97VGYY162 pKa = 10.04SSEE165 pKa = 4.1RR166 pKa = 11.84TFEE169 pKa = 4.26GDD171 pKa = 4.03LDD173 pKa = 3.92GTADD177 pKa = 4.83DD178 pKa = 6.39DD179 pKa = 5.19EE180 pKa = 7.72DD181 pKa = 5.7DD182 pKa = 5.2DD183 pKa = 4.07EE184 pKa = 7.14AGFLRR189 pKa = 11.84VASGDD194 pKa = 3.66FDD196 pKa = 3.82GLLYY200 pKa = 10.81SGLVGASYY208 pKa = 9.88QAKK211 pKa = 9.68LGQRR215 pKa = 11.84FTLTPLVSLEE225 pKa = 3.81YY226 pKa = 10.57FRR228 pKa = 11.84LDD230 pKa = 3.08EE231 pKa = 5.51DD232 pKa = 4.69GYY234 pKa = 11.04EE235 pKa = 4.11EE236 pKa = 4.25TGGGDD241 pKa = 3.52GMNLIVEE248 pKa = 4.6DD249 pKa = 4.54RR250 pKa = 11.84SSDD253 pKa = 3.65SLSINSTLALAYY265 pKa = 10.1AIGPQQPDD273 pKa = 4.5MIPMSIEE280 pKa = 3.38IEE282 pKa = 3.92AGRR285 pKa = 11.84RR286 pKa = 11.84TVLNGSLGDD295 pKa = 4.07TIAEE299 pKa = 4.15FEE301 pKa = 4.39DD302 pKa = 3.69VGDD305 pKa = 4.31SFRR308 pKa = 11.84LMAPDD313 pKa = 5.47IEE315 pKa = 4.64SAWLGEE321 pKa = 3.64ISIIGGGYY329 pKa = 10.27DD330 pKa = 3.86FLWSVNAAAEE340 pKa = 4.34HH341 pKa = 6.01EE342 pKa = 4.45GDD344 pKa = 3.29RR345 pKa = 11.84TVYY348 pKa = 10.32SLRR351 pKa = 11.84AGLGIAFF358 pKa = 4.81

Molecular weight: 38.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109LT76|A0A109LT76_9SPHN Uncharacterized protein OS=Erythrobacter sp. YT30 OX=1735012 GN=AUC45_04365 PE=4 SV=1
MM1 pKa = 6.85NTNLKK6 pKa = 10.4LFLSALTLAFVSTPVVAEE24 pKa = 3.81QVTVSVEE31 pKa = 3.99HH32 pKa = 7.39ADD34 pKa = 3.98LNLSNEE40 pKa = 4.17KK41 pKa = 10.69GIEE44 pKa = 4.11TLKK47 pKa = 10.83GRR49 pKa = 11.84LNGAINRR56 pKa = 11.84VCGPVGFRR64 pKa = 11.84SVTQIYY70 pKa = 9.54RR71 pKa = 11.84ARR73 pKa = 11.84KK74 pKa = 8.29CRR76 pKa = 11.84RR77 pKa = 11.84TLRR80 pKa = 11.84RR81 pKa = 11.84QARR84 pKa = 11.84MLLSEE89 pKa = 3.92IRR91 pKa = 11.84GGNVAIASNFEE102 pKa = 3.42IEE104 pKa = 4.21YY105 pKa = 10.56RR106 pKa = 11.84GG107 pKa = 3.37

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2997

0

2997

959551

42

2579

320.2

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.926 ± 0.053

0.818 ± 0.014

6.296 ± 0.034

6.731 ± 0.043

3.88 ± 0.029

8.531 ± 0.046

1.852 ± 0.021

5.431 ± 0.027

3.548 ± 0.037

9.456 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.026

2.946 ± 0.032

4.939 ± 0.033

3.226 ± 0.025

6.408 ± 0.047

5.789 ± 0.032

5.31 ± 0.036

6.812 ± 0.037

1.369 ± 0.02

2.222 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski