Pisciglobus halotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Pisciglobus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3BED7|A0A1I3BED7_9LACT Predicted membrane protein OS=Pisciglobus halotolerans OX=745365 GN=SAMN04489868_10611 PE=4 SV=1
MM1 pKa = 7.17AHH3 pKa = 5.99EE4 pKa = 5.24HH5 pKa = 5.84DD6 pKa = 4.78HH7 pKa = 5.92EE8 pKa = 5.92HH9 pKa = 5.9GHH11 pKa = 5.31DD12 pKa = 3.85HH13 pKa = 5.25EE14 pKa = 6.32HH15 pKa = 5.5ITIVDD20 pKa = 3.6EE21 pKa = 4.19QGNEE25 pKa = 3.71EE26 pKa = 4.4LYY28 pKa = 10.85EE29 pKa = 3.9ILFTFDD35 pKa = 3.6SDD37 pKa = 4.57DD38 pKa = 3.75FEE40 pKa = 5.52KK41 pKa = 10.82SYY43 pKa = 11.56VLVYY47 pKa = 9.69PAGAAEE53 pKa = 4.25GEE55 pKa = 4.35EE56 pKa = 4.46VEE58 pKa = 5.22LSAFSFIQDD67 pKa = 3.37EE68 pKa = 4.31NGEE71 pKa = 4.15QGQLNPIEE79 pKa = 5.08TEE81 pKa = 4.27AEE83 pKa = 3.18WDD85 pKa = 3.67MIEE88 pKa = 4.3EE89 pKa = 4.14VLNTFMEE96 pKa = 4.73DD97 pKa = 3.17EE98 pKa = 4.4EE99 pKa = 4.49EE100 pKa = 4.06

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3DV49|A0A1I3DV49_9LACT Site-specific DNA-methyltransferase (adenine-specific) OS=Pisciglobus halotolerans OX=745365 GN=SAMN04489868_1536 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84NVLQSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.58GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2245

0

2245

660971

8

1445

294.4

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.315 ± 0.052

0.529 ± 0.013

5.444 ± 0.044

7.798 ± 0.071

4.437 ± 0.047

6.492 ± 0.05

1.976 ± 0.026

7.463 ± 0.052

7.12 ± 0.051

9.695 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.024

4.364 ± 0.034

3.396 ± 0.031

4.291 ± 0.039

3.869 ± 0.039

5.997 ± 0.046

5.751 ± 0.036

6.786 ± 0.045

0.912 ± 0.019

3.608 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski