Salibacterium qingdaonense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salibacterium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4PXI8|A0A1I4PXI8_9BACI Two-component system CitB family response regulator MalR OS=Salibacterium qingdaonense OX=266892 GN=SAMN04488054_13316 PE=4 SV=1
MM1 pKa = 7.18FKK3 pKa = 10.24KK4 pKa = 10.51VSVFIIVSFALLLSACGSSGSSSEE28 pKa = 4.4EE29 pKa = 3.67NASNYY34 pKa = 9.71PEE36 pKa = 3.99NQIEE40 pKa = 4.55VIVPFSSGGASDD52 pKa = 4.05LVSRR56 pKa = 11.84TVASEE61 pKa = 3.94MEE63 pKa = 4.17NDD65 pKa = 3.6LDD67 pKa = 4.5VPLTITNQTGGSGATGMLSLKK88 pKa = 10.04NAQADD93 pKa = 4.78GYY95 pKa = 8.4TIGYY99 pKa = 8.9VPVEE103 pKa = 3.92MSMLDD108 pKa = 3.62SLEE111 pKa = 4.4LADD114 pKa = 4.54ITPSDD119 pKa = 4.06YY120 pKa = 11.49EE121 pKa = 4.51FIGQLMTIPSAITVPADD138 pKa = 3.2APYY141 pKa = 9.23DD142 pKa = 3.81TIEE145 pKa = 4.26EE146 pKa = 4.22FVSYY150 pKa = 11.09AEE152 pKa = 4.29EE153 pKa = 4.28NPGEE157 pKa = 4.05IQIGNSGTGSIWHH170 pKa = 6.63IAAAAFAEE178 pKa = 4.34EE179 pKa = 4.06AGIDD183 pKa = 3.95VEE185 pKa = 4.34YY186 pKa = 11.02VPYY189 pKa = 10.69DD190 pKa = 3.56GASPAVTALMGGHH203 pKa = 6.81ISAVSVSPSEE213 pKa = 3.87VRR215 pKa = 11.84GGVEE219 pKa = 4.11SGDD222 pKa = 3.73LKK224 pKa = 11.23VLGVMGEE231 pKa = 4.07EE232 pKa = 4.58RR233 pKa = 11.84DD234 pKa = 3.84PEE236 pKa = 4.7LPDD239 pKa = 3.3VPTMQEE245 pKa = 2.98AGYY248 pKa = 9.72DD249 pKa = 3.54VSVAGWGGFVAPEE262 pKa = 4.02GTPEE266 pKa = 3.99EE267 pKa = 4.16VLEE270 pKa = 4.16PLRR273 pKa = 11.84SSFKK277 pKa = 10.38NAAEE281 pKa = 4.08SEE283 pKa = 4.32EE284 pKa = 4.22FQQLMDD290 pKa = 4.45DD291 pKa = 3.75RR292 pKa = 11.84GMIPAYY298 pKa = 9.96KK299 pKa = 9.89NGKK302 pKa = 8.57EE303 pKa = 3.85FSSYY307 pKa = 11.39AEE309 pKa = 3.67EE310 pKa = 4.22QYY312 pKa = 11.43DD313 pKa = 4.04YY314 pKa = 10.87FSEE317 pKa = 5.1LIPTIEE323 pKa = 4.13INQQ326 pKa = 3.46

Molecular weight: 34.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4NBL4|A0A1I4NBL4_9BACI Cyclic-di-AMP phosphodiesterase OS=Salibacterium qingdaonense OX=266892 GN=SAMN04488054_11658 PE=3 SV=1
MM1 pKa = 7.66GKK3 pKa = 8.01PTFSPNNRR11 pKa = 11.84KK12 pKa = 9.29RR13 pKa = 11.84KK14 pKa = 8.42KK15 pKa = 8.43VHH17 pKa = 5.59GFRR20 pKa = 11.84EE21 pKa = 3.98RR22 pKa = 11.84MSTKK26 pKa = 10.18KK27 pKa = 10.45GRR29 pKa = 11.84QVLKK33 pKa = 9.7NRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.34GRR40 pKa = 11.84KK41 pKa = 9.01VISAA45 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3532

0

3532

1002558

39

1531

283.8

31.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.837 ± 0.043

0.67 ± 0.013

5.494 ± 0.042

8.263 ± 0.06

4.259 ± 0.039

7.31 ± 0.044

2.312 ± 0.024

6.386 ± 0.04

5.309 ± 0.039

9.247 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.023

3.869 ± 0.031

3.882 ± 0.025

4.043 ± 0.031

4.701 ± 0.036

6.216 ± 0.03

5.653 ± 0.031

7.036 ± 0.035

1.134 ± 0.018

3.305 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski