Yellowstone Lake virophage 7

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RL93|A0A0A0RL93_9VIRU Uncharacterized protein OS=Yellowstone Lake virophage 7 OX=1557035 GN=YSLV7_ORF21 PE=4 SV=1
MM1 pKa = 6.89SAKK4 pKa = 9.95TIFPGGNTQFLPGLAGLVGSTPPAISTSSINCQTGEE40 pKa = 4.14LQAATAQIGFGSAPGEE56 pKa = 4.25IILQVGNPSGTNANFSIGTGVGNPLTIARR85 pKa = 11.84GISASSGNITAPTGTINAFNGITTTNGGFIATAGGLTCTSGEE127 pKa = 4.17VQAATAQIGFGSTTGDD143 pKa = 3.38VIFQVGNPDD152 pKa = 3.35DD153 pKa = 3.77VAVNFSVGAGAGNPLTIGSGLTCTAGGLTCASGEE187 pKa = 4.36VVAATAQIGAGSTTGDD203 pKa = 3.43VIFQVGNPDD212 pKa = 3.35DD213 pKa = 3.77VAVNFSVGTGAGNPLTIGSAIKK235 pKa = 10.43AAGLVDD241 pKa = 4.51ANGSTGEE248 pKa = 4.02NGQYY252 pKa = 10.41PIANGTGGFVWTTPP266 pKa = 3.22

Molecular weight: 25.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RS45|A0A0A0RS45_9VIRU Uncharacterized protein OS=Yellowstone Lake virophage 7 OX=1557035 GN=YSLV7_ORF05 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84IIAYY6 pKa = 7.89MFFLCGFLYY15 pKa = 10.74VFYY18 pKa = 10.53FLGKK22 pKa = 10.13RR23 pKa = 11.84GIQNFYY29 pKa = 9.57YY30 pKa = 10.63KK31 pKa = 10.22NISVSPFFPKK41 pKa = 10.5NKK43 pKa = 9.81NKK45 pKa = 10.39NKK47 pKa = 9.98YY48 pKa = 8.87IYY50 pKa = 10.35AIIQMTKK57 pKa = 10.08

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

6488

56

922

249.5

28.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.502 ± 0.693

1.418 ± 0.235

5.348 ± 0.519

5.626 ± 0.758

4.377 ± 0.303

6.119 ± 0.886

1.279 ± 0.248

7.753 ± 0.413

8.755 ± 1.061

8.431 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.236

7.414 ± 0.44

4.023 ± 0.447

3.699 ± 0.334

3.483 ± 0.433

6.89 ± 0.697

6.72 ± 0.691

4.624 ± 0.243

0.909 ± 0.151

5.194 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski