Microbacterium phage Count

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UIJ6|A0A2U8UIJ6_9CAUD DNA binding protein OS=Microbacterium phage Count OX=2182352 GN=119 PE=4 SV=2
MM1 pKa = 7.57GEE3 pKa = 4.09VAHH6 pKa = 6.61AASDD10 pKa = 4.04RR11 pKa = 11.84YY12 pKa = 9.73PRR14 pKa = 11.84EE15 pKa = 4.0ILDD18 pKa = 3.5EE19 pKa = 5.36DD20 pKa = 4.53YY21 pKa = 11.65DD22 pKa = 5.04DD23 pKa = 4.18GTMPASLDD31 pKa = 3.56ALGEE35 pKa = 3.95AVIGHH40 pKa = 6.86KK41 pKa = 9.97IVEE44 pKa = 4.2VRR46 pKa = 11.84HH47 pKa = 6.15GSFKK51 pKa = 10.56PPKK54 pKa = 9.54AWWPQTGLALLLDD67 pKa = 4.22NGHH70 pKa = 6.22IVWMAEE76 pKa = 3.8EE77 pKa = 5.08GDD79 pKa = 3.92CCAYY83 pKa = 9.94TSVEE87 pKa = 3.41VDD89 pKa = 3.71TIIKK93 pKa = 10.33HH94 pKa = 6.16IDD96 pKa = 3.57QIDD99 pKa = 3.66HH100 pKa = 6.6VITGVGTTDD109 pKa = 5.82GYY111 pKa = 7.32TQWHH115 pKa = 7.04IYY117 pKa = 10.43ADD119 pKa = 3.88LGDD122 pKa = 3.74VLEE125 pKa = 5.52FEE127 pKa = 5.25VGWSCGNPFYY137 pKa = 10.73YY138 pKa = 10.3AYY140 pKa = 10.27GFNITVFEE148 pKa = 4.61PNQEE152 pKa = 4.06GLTEE156 pKa = 4.11AFKK159 pKa = 10.83EE160 pKa = 4.24LEE162 pKa = 4.08RR163 pKa = 5.9

Molecular weight: 18.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UI76|A0A2U8UI76_9CAUD Acetyltransferase OS=Microbacterium phage Count OX=2182352 GN=42 PE=4 SV=1
MM1 pKa = 7.6VDD3 pKa = 3.28YY4 pKa = 10.2TKK6 pKa = 10.55ATGSGGLMMIRR17 pKa = 11.84DD18 pKa = 3.99LGSNVEE24 pKa = 3.69FHH26 pKa = 6.43IRR28 pKa = 11.84AGFDD32 pKa = 3.04STYY35 pKa = 10.59INQLRR40 pKa = 11.84WKK42 pKa = 9.74RR43 pKa = 11.84YY44 pKa = 8.93VNGSWSGLLGPVAYY58 pKa = 7.68PTGRR62 pKa = 11.84PWKK65 pKa = 9.93KK66 pKa = 9.87LDD68 pKa = 3.45SFKK71 pKa = 11.28VSGNQNICFRR81 pKa = 11.84LEE83 pKa = 3.97GTGTQGLGGPTEE95 pKa = 4.58FWQTINRR102 pKa = 11.84ATIPPAPTPLSLDD115 pKa = 3.83QIGHH119 pKa = 4.98TSVRR123 pKa = 11.84YY124 pKa = 9.48RR125 pKa = 11.84FQSTGNGGSPILEE138 pKa = 3.95WQIGYY143 pKa = 9.9GKK145 pKa = 10.28SPTSVQFTKK154 pKa = 10.45KK155 pKa = 10.33SSGTSVVDD163 pKa = 3.67GLTLGDD169 pKa = 3.97RR170 pKa = 11.84WYY172 pKa = 10.41FWARR176 pKa = 11.84GRR178 pKa = 11.84NAVGWGPWSARR189 pKa = 11.84MDD191 pKa = 3.37ARR193 pKa = 11.84TLAGARR199 pKa = 11.84VLVSGTWRR207 pKa = 11.84EE208 pKa = 3.96AVPMVRR214 pKa = 11.84VAGVWRR220 pKa = 11.84QAIPFIRR227 pKa = 11.84VSGAWKK233 pKa = 10.25DD234 pKa = 3.85GKK236 pKa = 10.7

Molecular weight: 26.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

131

0

131

24597

38

1417

187.8

20.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.298 ± 0.365

0.553 ± 0.082

6.338 ± 0.219

7.033 ± 0.359

3.838 ± 0.216

7.326 ± 0.295

1.858 ± 0.145

5.997 ± 0.182

5.891 ± 0.32

7.81 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.671 ± 0.181

4.484 ± 0.159

3.996 ± 0.181

3.297 ± 0.13

5.566 ± 0.336

6.403 ± 0.322

6.639 ± 0.42

7.005 ± 0.217

1.61 ± 0.108

3.387 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski