Ellagibacter isourolithinifaciens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Ellagibacter

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6NPH1|A0A6N6NPH1_9ACTN Phosphate acyltransferase OS=Ellagibacter isourolithinifaciens OX=2137581 GN=plsX PE=3 SV=1
MM1 pKa = 8.16DD2 pKa = 4.72EE3 pKa = 4.35LSSVRR8 pKa = 11.84SSLRR12 pKa = 11.84VVSGDD17 pKa = 3.63PLSLEE22 pKa = 4.23VPAEE26 pKa = 3.98LFSPAEE32 pKa = 3.88FAQFEE37 pKa = 4.61GTVAVDD43 pKa = 3.75EE44 pKa = 5.2FEE46 pKa = 5.2CGPDD50 pKa = 2.91SYY52 pKa = 11.28RR53 pKa = 11.84FSEE56 pKa = 4.28PLSWHH61 pKa = 6.79ADD63 pKa = 3.23VSNTGDD69 pKa = 3.52ALLVTGNVTGEE80 pKa = 3.84ARR82 pKa = 11.84TDD84 pKa = 3.49CARR87 pKa = 11.84CLEE90 pKa = 4.42DD91 pKa = 4.17AVFDD95 pKa = 3.71IEE97 pKa = 5.47GEE99 pKa = 3.98IEE101 pKa = 5.16GYY103 pKa = 10.28FLLDD107 pKa = 3.25GAEE110 pKa = 4.05IPEE113 pKa = 4.59GADD116 pKa = 2.96EE117 pKa = 5.61DD118 pKa = 4.13EE119 pKa = 4.87FDD121 pKa = 4.39RR122 pKa = 11.84LPANHH127 pKa = 6.24VLDD130 pKa = 3.99MEE132 pKa = 4.79SLVVAAIVFEE142 pKa = 4.53LPLVPLCDD150 pKa = 3.96EE151 pKa = 4.68DD152 pKa = 5.77CKK154 pKa = 11.23GLCPTCGANLNEE166 pKa = 4.82GPCGCEE172 pKa = 4.04KK173 pKa = 10.4EE174 pKa = 4.67QSDD177 pKa = 4.66GPDD180 pKa = 3.11EE181 pKa = 5.09FEE183 pKa = 4.19LAKK186 pKa = 11.12NPFAALANFSFDD198 pKa = 3.45SDD200 pKa = 3.53EE201 pKa = 4.51SADD204 pKa = 3.54

Molecular weight: 21.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6NJ98|A0A6N6NJ98_9ACTN Uncharacterized protein OS=Ellagibacter isourolithinifaciens OX=2137581 GN=F8C90_09595 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.41RR3 pKa = 11.84SDD5 pKa = 3.67TRR7 pKa = 11.84WKK9 pKa = 8.92PKK11 pKa = 8.77RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84ARR19 pKa = 11.84RR20 pKa = 11.84ARR22 pKa = 11.84RR23 pKa = 11.84PPPRR27 pKa = 11.84KK28 pKa = 8.93PRR30 pKa = 11.84SPPSRR35 pKa = 11.84RR36 pKa = 11.84PKK38 pKa = 10.12RR39 pKa = 11.84LRR41 pKa = 11.84PNPPSRR47 pKa = 11.84RR48 pKa = 11.84PKK50 pKa = 9.88RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84PSPRR57 pKa = 11.84SNGARR62 pKa = 11.84HH63 pKa = 4.66WRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84PSRR70 pKa = 11.84WATSRR75 pKa = 11.84ASTCARR81 pKa = 11.84RR82 pKa = 11.84SRR84 pKa = 11.84SNRR87 pKa = 11.84RR88 pKa = 11.84SSGPSPTTAPP98 pKa = 3.62

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2050

0

2050

699948

23

2285

341.4

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.313 ± 0.078

1.749 ± 0.024

6.012 ± 0.045

6.838 ± 0.061

3.922 ± 0.038

8.065 ± 0.05

1.822 ± 0.022

5.319 ± 0.044

4.356 ± 0.044

8.846 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.028

3.043 ± 0.032

4.263 ± 0.032

2.75 ± 0.026

5.896 ± 0.058

6.215 ± 0.049

5.175 ± 0.045

7.821 ± 0.043

1.094 ± 0.022

2.823 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski