Buchnera aphidicola subsp. Cinara cedri (strain Cc)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola; Buchnera aphidicola (Cinara cedri)

Average proteome isoelectric point is 8.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q057M1|RSMC_BUCCC Ribosomal RNA small subunit methyltransferase C OS=Buchnera aphidicola subsp. Cinara cedri (strain Cc) OX=372461 GN=rsmC PE=3 SV=1
MM1 pKa = 7.35NNIINSIITLDD12 pKa = 3.64DD13 pKa = 3.79RR14 pKa = 11.84NLISKK19 pKa = 7.48EE20 pKa = 4.05TIINQDD26 pKa = 2.9YY27 pKa = 11.5DD28 pKa = 4.07NIDD31 pKa = 3.25TEE33 pKa = 4.92FYY35 pKa = 10.79KK36 pKa = 10.63KK37 pKa = 10.24YY38 pKa = 10.64AYY40 pKa = 10.28NIQTNSSDD48 pKa = 4.16DD49 pKa = 3.5QITNKK54 pKa = 10.34KK55 pKa = 9.65IVDD58 pKa = 3.63INSDD62 pKa = 4.18DD63 pKa = 3.9NSSLQSYY70 pKa = 9.87SNSSIDD76 pKa = 3.62EE77 pKa = 4.16DD78 pKa = 4.76TEE80 pKa = 4.47TGWFSVFDD88 pKa = 4.25DD89 pKa = 4.42DD90 pKa = 5.72GYY92 pKa = 11.48EE93 pKa = 3.98GFLRR97 pKa = 11.84EE98 pKa = 3.61GHH100 pKa = 6.07IAINEE105 pKa = 3.79NRR107 pKa = 11.84EE108 pKa = 3.98LCMNNYY114 pKa = 8.34TLLNTDD120 pKa = 4.04EE121 pKa = 4.88KK122 pKa = 11.01PIIVPEE128 pKa = 4.02NKK130 pKa = 10.01KK131 pKa = 10.14ILIKK135 pKa = 9.88MDD137 pKa = 3.06GTIVTKK143 pKa = 10.61EE144 pKa = 3.91DD145 pKa = 3.48NNEE148 pKa = 3.67INYY151 pKa = 9.81KK152 pKa = 10.36PIFLGQLKK160 pKa = 10.58FITLNNNDD168 pKa = 3.52VISRR172 pKa = 11.84NEE174 pKa = 3.76SEE176 pKa = 5.32IFDD179 pKa = 4.08LSYY182 pKa = 11.42SGLEE186 pKa = 3.83KK187 pKa = 10.96YY188 pKa = 10.53KK189 pKa = 11.18NNNLEE194 pKa = 4.17TKK196 pKa = 10.45SNYY199 pKa = 8.06VLKK202 pKa = 9.8NTIRR206 pKa = 11.84KK207 pKa = 9.21NDD209 pKa = 3.18IKK211 pKa = 10.56KK212 pKa = 8.92TNNLINTLNVIRR224 pKa = 11.84SLEE227 pKa = 3.89SDD229 pKa = 3.36MKK231 pKa = 10.97INSS234 pKa = 3.43

Molecular weight: 27.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q058F9|DNAA_BUCCC Chromosomal replication initiator protein DnaA OS=Buchnera aphidicola subsp. Cinara cedri (strain Cc) OX=372461 GN=dnaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.0SGRR28 pKa = 11.84NIISHH33 pKa = 6.31RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.64LRR39 pKa = 11.84SVLCVV44 pKa = 3.07

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

362

0

362

119513

38

1410

330.1

38.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.339 ± 0.119

1.279 ± 0.046

3.568 ± 0.085

4.266 ± 0.115

5.811 ± 0.178

4.794 ± 0.115

1.82 ± 0.043

13.394 ± 0.18

13.59 ± 0.294

9.636 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.046

8.118 ± 0.126

2.681 ± 0.063

2.7 ± 0.056

3.199 ± 0.108

6.943 ± 0.089

4.092 ± 0.081

3.79 ± 0.11

0.817 ± 0.046

4.304 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski