Streptomyces virus TG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tigunavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4IBQ2|K4IBQ2_9CAUD Thioredoxin OS=Streptomyces virus TG1 OX=374422 GN=TG1_39 PE=4 SV=1
MM1 pKa = 7.25WRR3 pKa = 11.84VAHH6 pKa = 6.87EE7 pKa = 3.97SARR10 pKa = 11.84YY11 pKa = 9.34NGFPAGPYY19 pKa = 9.41ACSDD23 pKa = 3.78ALPTRR28 pKa = 11.84SVSILFHH35 pKa = 6.48MGLEE39 pKa = 4.43HH40 pKa = 7.32SGTDD44 pKa = 3.34HH45 pKa = 7.44PSPYY49 pKa = 10.3ADD51 pKa = 3.49PALRR55 pKa = 11.84GIADD59 pKa = 3.94FEE61 pKa = 4.52RR62 pKa = 11.84CGFEE66 pKa = 4.51SRR68 pKa = 11.84EE69 pKa = 3.99ALNAWFDD76 pKa = 3.65GWSEE80 pKa = 4.11ALDD83 pKa = 3.79EE84 pKa = 4.7AGMRR88 pKa = 11.84VWVYY92 pKa = 10.62DD93 pKa = 3.62VPDD96 pKa = 3.2WCARR100 pKa = 11.84SGFAGQVVFDD110 pKa = 4.12ALEE113 pKa = 4.13AVEE116 pKa = 4.0VDD118 pKa = 4.01AYY120 pKa = 10.83AFEE123 pKa = 4.87PEE125 pKa = 4.2QLSLFF130 pKa = 4.15

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I0E9|K4I0E9_9CAUD Uncharacterized protein OS=Streptomyces virus TG1 OX=374422 GN=TG1_53 PE=4 SV=1
MM1 pKa = 7.16SHH3 pKa = 7.09SEE5 pKa = 4.16VQRR8 pKa = 11.84TTQNAPRR15 pKa = 11.84FAVGQQSGALDD26 pKa = 3.41TKK28 pKa = 10.98GRR30 pKa = 11.84VLVAKK35 pKa = 10.41CMGGRR40 pKa = 11.84CVCCLRR46 pKa = 11.84RR47 pKa = 11.84VKK49 pKa = 10.53QLFPVPYY56 pKa = 9.14RR57 pKa = 11.84VTAKK61 pKa = 10.23VVVIRR66 pKa = 11.84PYY68 pKa = 10.9CGGCAPAKK76 pKa = 10.52AKK78 pKa = 10.48GAAA81 pKa = 3.43

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12256

50

801

227.0

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.753 ± 0.426

0.93 ± 0.157

6.666 ± 0.285

6.111 ± 0.367

3.223 ± 0.197

8.877 ± 0.414

1.73 ± 0.148

3.256 ± 0.24

3.949 ± 0.301

7.841 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.116

2.676 ± 0.14

5.573 ± 0.364

2.538 ± 0.175

7.05 ± 0.432

5.508 ± 0.235

6.552 ± 0.335

8.273 ± 0.276

1.82 ± 0.149

2.635 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski