Escherichia phage Rac-SA53

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U2QV81|A0A0U2QV81_9CAUD Modulator of killing protein OS=Escherichia phage Rac-SA53 OX=1759531 GN=mok PE=4 SV=1
MM1 pKa = 7.38NEE3 pKa = 3.27IRR5 pKa = 11.84EE6 pKa = 4.37IPVEE10 pKa = 3.96RR11 pKa = 11.84DD12 pKa = 3.08EE13 pKa = 4.49YY14 pKa = 11.16GCWAHH19 pKa = 7.14PEE21 pKa = 4.02YY22 pKa = 10.99EE23 pKa = 4.29KK24 pKa = 11.16FCDD27 pKa = 3.26GRR29 pKa = 11.84EE30 pKa = 4.11YY31 pKa = 10.62ISTEE35 pKa = 3.75EE36 pKa = 3.62FNAWMEE42 pKa = 4.36EE43 pKa = 3.93NNLQYY48 pKa = 10.86VLCFRR53 pKa = 11.84DD54 pKa = 3.98EE55 pKa = 4.23GCADD59 pKa = 5.1LDD61 pKa = 4.13ACDD64 pKa = 4.63ADD66 pKa = 3.47ISAWEE71 pKa = 4.09PEE73 pKa = 4.14RR74 pKa = 11.84PEE76 pKa = 4.09GNGWFIGSIHH86 pKa = 6.51DD87 pKa = 4.01TEE89 pKa = 5.32DD90 pKa = 3.84GPVCVWLRR98 pKa = 11.84NKK100 pKa = 10.64AEE102 pKa = 3.86AA103 pKa = 4.16

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2KD19|A0A0U2KD19_9CAUD Uncharacterized protein OS=Escherichia phage Rac-SA53 OX=1759531 PE=4 SV=1
MM1 pKa = 7.69LGNLPQLLNGALCTIIVLTLFFYY24 pKa = 10.47RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.6GATYY31 pKa = 10.12KK32 pKa = 10.76PLISWLAWLLMLFYY46 pKa = 10.97AFAPLRR52 pKa = 11.84HH53 pKa = 6.29LYY55 pKa = 9.27GHH57 pKa = 6.95PVPANWLVVAVNLLFCALVICVRR80 pKa = 11.84GNVSKK85 pKa = 11.04LLLLRR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 3.9

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

15938

34

1061

227.7

25.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.539 ± 0.429

1.305 ± 0.109

5.408 ± 0.217

6.745 ± 0.422

3.539 ± 0.175

7.027 ± 0.366

1.694 ± 0.17

6.168 ± 0.237

5.59 ± 0.258

8.508 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.154

4.875 ± 0.236

4.543 ± 0.166

4.204 ± 0.166

5.358 ± 0.297

6.55 ± 0.252

5.898 ± 0.224

6.563 ± 0.27

1.462 ± 0.098

3.589 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski